A tissue staining method which comprises: staining a tissue with a staining reagent wherein a biosubstance recognition site is bonded to particles carrying multiple fluorescent substances accumulated therein; in the stained tissue, counting fluorescent points or measuring fluorescent brightness; and evaluating the expression level of a biosubstance, which matches the biosubstance recognition site, in the aforesaid tissue on the basis of the number of the fluorescent points or fluorescent brightness that was measured.

Self-assembled structures formed of a plurality of cyclic peptides which are in association with metal ions is provided. The cyclic peptides are each of from 2 to 6 amino acid residues, and two or more of the amino acid residues are each independently an aromatic amino acid residue. The self-assembled structures exhibit photoluminescence and can be used or incorporated in light emitting systems.

The present invention provides a biological use including a bioplatform and a method for detecting a high-sensitivity biomolecule by providing improved quantum yield (QY) and brightness when detecting biomolecules by preparing nanoparticles, which are doped in multiple quantum dots and have a structure of inorganic core particles, a quantum dot buried layer and a silica/quantum dot composite shell, while maintaining a high occupancy and stable bonding.

An apparatus for capturing biological material. The apparatus includes: an optically readable capture particle (ORCP) including: one or more optically readable particles (ORPs) each including an optical barcode to identify the ORCP; and a plurality of biological capture sites associated with the one or more ORPs, each of the plurality of biological capture sites including a cellular barcode to identify the ORCP.

An assay device is provided for use in determining the presence of a target analyte in a sample. The assay device comprises a solid platform comprising a fibrous mat, the solid platform impregnated with a first FRET chromophore. An antibody-FRET chromophore conjugate is immobilized on a surface of the solid platform, wherein the antibody-FRET chromophore conjugate comprises an antibody affixed to a second FRET chromophore. The first FRET chromophore and the second FRET chromophore are selected to provide an energy transfer from one to another when located within a Förster distance with respect to each other, thereby forming a FRET donor-acceptor chromophore pair. In a further aspect, a method of detecting a target analyte in a sample is provided. In yet a further aspect, packaging sheet materials and packaging articles employing the assay device under certain conditions are provided.

The present disclosure provides a reagent kit for detecting a sex hormone, which contains a first reagent containing a metal nanoprobe in which a sex hormone and a Raman reporter are immobilized and a second reagent containing a magnetic particle in which an antibody for detecting the sex hormone is immobilized, and a method for detecting a sex hormone using the same.

Apparatus and methods for the detection of proteins in biological fluids such as urine using a label-free assay is described. Specific proteins are detected by their binding to highly specific capture reagents such as SOMAmers that are attached to the surface of a substrate. Changes to these capture reagents and their local environment upon protein binding modify the behavior of color centers (e.g., fluorescence, ionization state, spin state, etc.) embedded in the substrate beneath the bound capture reagents. These changes can be read out, for example, optically or electrically, for an individual color center or as an average response of many color centers.

Protected quantum dots are protected from degradation, particularly in aqueous environments, The system comprises quantum dots, hydrophobic core, and hydrophilic shell. The quantum dots are entrapped in and protected by the hydrophobic core. The core polymer is covalently bonded to a hydrophilic shell polymer or protein. Quantum yield is better maintained than for non-encapsulated quantum dots in an aqueous environment. Optionally, ligands are attached to the hydrophilic shell to target delivery of the protected quantum dots, In an alternative embodiment, quantum dots are entrapped in the hydrophilic shell, or in both the shell and the core.

The present invention relates to a standard material composition for verifying a bio-analysis equipment, comprising quantum dot-containing nanoparticles. Through a standard strip and/or a standard tray, which are made of the standard material composition, the present invention may increase the analysis accuracy of a bio-analysis equipment.

An assay substrate including a first component comprising a sensor molecule labeled with a quantum dot, the quantum dot immobilized to an assay substrate surface with a first linker being a bi-polar linker comprising a first binding group for specific binding of the quantum dot and a second binding group for specific binding of the assay substrate surface, the sensor molecule having a specific binding site for an organic analyte, the sensor molecule labeled with the quantum dot in a position that has no effect on the organic analyte binding the specific binding site; and a second component comprising a chemical analogue of the organic analyte, the chemical analogue labeled with a fluorescent dye, the chemical analogue linked to the quantum dot with a second linker having a length exceeding Foster radius, and the chemical analogue reversibly binding the specific binding site of the sensor molecule of the first component.