A reagent for use in the measurement of hemoglobins by liquid chromatography, the reagent comprising a nonionic surfactant selected from the group consisting of:

(i) polyoxyethylene (10) decyl ether; (ii) polyoxyethylene (6) 2-ethylhexyl ether; (iii) polyoxyethylene (9) isodecyl ether; (iv) polyoxyethylene (10) nonyl ether; (v) polyoxyethylene (16) isostearyl ether; (vi) polyoxyethylene (20) behenyl ether; and (vii) polyoxyethylene (20) polyoxypropylene (6) decyltetradecyl ether.

The present invention relates to a method for quantitative measurement of catechol estrogen bound protein in blood sample. By detecting adduction levels of binding sites of the catechol estrogen on the protein in blood sample, the catechol estrogen bound protein in the blood sample can be detected quantitatively and a limit of quantitation can be decreased.

The present invention relates to methods and compositions for quantifying hemoglobin using, inter alia, a peroxidase substrate and hydrogen peroxide.

A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.

Disclosed herein are potency assays for a gene therapy treatment for β-thalassemia. Also disclosed herein are methods for measuring relative potency of a drug product.

The present application provides an occult blood detection film, a preparation method therefor, a use thereof and an occult blood detection kit. The preparation method for the occult blood detection film comprises the following steps: providing a polyionic film; immersing the polyionic film into a dye solution, so that the dye is attached to the polyionic film to obtain the occult blood detection film, where the dye is suitable for reacting with hemoglobin, the colors of the dye are different in environments with different hemoglobin concentrations, and the dye comprises at least one of a methylene blue dye or a procyanidin dye. The present application has the characteristics of easy operation, strong anti-interference performance, obvious color change, high detection speed, etc., and can alleviate the problems of high technical requirements, high cost and complicated detection.

Provided herein are methods for accommodating for sample matrix effects in light measurement assays. A selected amount of a light emitting material is added to a sample, and the light output from the sample is measured. By using an algorithm based on the known light emitting material amount and the measured light output, a correction factor for the assay of the sample is determined. The correction factor can be used to adjust subsequent light output measurements of other samples recorded using the assay.

An example system includes one or more non-transitory machine-readable media and one or more processors. The non-transitory machine-readable media is configured to store data and instructions, in which the data includes image data having a plurality of image frames of an electrophoresis process performed on a sample over a time interval. The sample contains at least one analyte. The one or more processors are configured to access the data and execute the instructions, in which the instructions programmed to perform a method. The method can include determining values of pixels within a region of interest (ROI) of respective image frames in the time interval. The method can also include analyzing the determined pixel values for at least some of the respective image frames. The method can also include estimating a quantity of the at least one analyte in the sample based on the analysis of the pixel values.

The present invention relates to an improved method for the screening, diagnosis and/or monitoring of colorectal advanced neoplasia (AN), advanced adenoma (AA) and/or colorectal cancer (CRC), wherein AN encompasses CRC and AA. In particular, said method provides an increase of specificity due to a reduction of false positive results in the fecal occult blood test (FOBT) using a signature based on bacterial markers. It further relates to the use of said method in the selection of subjects for conducting an exploratory test (e.g., a colonoscopy) or for the treatment with an anti-cancer therapy.

A method for assessing an effect of hypoxia on a tissue includes providing a sample of the tissue in a hermetically sealed container, determining a first amount of a reaction substrate (e.g., protocatechuic acid) to be introduced into the sealed container and determining a second amount of a reaction enzyme (e.g., protocatechuate dioxygenase) to be introduced into the sealed container. The method further includes introducing the reaction substrate and the reaction enzyme into the sealed container. At least one of the first amount of the reaction substrate and the second amount of the reaction enzyme is selected to induce at least one of a predetermined amount of hypoxia less than anoxia and a predetermined rate of hypoxia in the tissue during a reaction between the reaction substrate and the reaction enzyme. Values of properties of the tissue can be measured before and after the reaction to assess effects of hypoxia.