A porous mirror (1) for detection of an analyte (96) in a fluid (99) by optical probing, comprising a translucent slab (2) with a front side (3), and a backside (4) facing away from the front side (3), wherein the front side (3) is adapted for being contacted with a fluid (99), and a reflective layer (5) at the front side (3) of the translucent slab (2), the reflective layer (5) being adapted to reflect light reaching the reflective layer from the backside (4) of the translucent slab (2), wherein the translucent slab (2) comprises pores (6), wherein the pores (6) are dead end pores (6) extending from respective openings (7) at the front side (3) into the translucent slab (2), through the reflective layer (5), wherein a cross-sectional dimension of the openings (7) of the pores (6) is dimensioned so as to prevent larger particles or debris, if any included the fluid, from entering the pores (6), while allowing the analyte (96) in the fluid (99) to enter the pores (6) via diffusion.

Sensor for the optical detection of free hemoglobin (96) in a whole blood sample (99), the sensor comprising a translucent slab (2) with a front side (3) and a back side (4) facing away from the front side (3), wherein the front side (3) is adapted for being contacted with a whole blood sample (99); a reflective layer (5) at the front side (3) of the translucent slab (2), the reflective layer (5) being adapted to reflect light reaching the reflective layer (5) from the translucent slab (2); an optical probing device comprising a light source (10) and a detector (20), wherein the light source (10) is adapted to illuminate at least pores in the translucent slab, wherein the detector (20) is arranged to receive light (21) emerging from the pores (6) in response to an illumination (11) by the light source (10), and wherein the detector (20) is adapted to generate a signal representative of the detected light. The translucent slab (2) is provided with dead-end pores (6) extending from the front side (3) into the translucent slab (2) in a direction towards the backside (4). Each of the pores (6) has a respective opening (7) in the front side (3) of the translucent slab (2) penetrating the reflecting layer (5). A cross-sectional dimension of the openings (7) of the pores (6) is dimensioned so as to prevent red blood cells (98) from entering the pores (6), while allowing free hemoglobin (96) to enter the pores (6).

The present invention provides a water soluble polymer surfactant (PS-DGlu) of formula I which is utilized for synthesis of functionalized polystyrene nanobead covalently incorporating (oligo) p-phenylenevinylene (OPV) nanosensor (PSG-OPV-n) which in turn is useful for the detection of bilirubin in human serum. The present invention further provides a process for the preparation of the water soluble polymer surfactant (PS-DGlu) of formula (I) and a mini emulsion polymerization process for the synthesis of PSG-OPV-n.

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wherein, n is 30-50.

The PSG-OPV-n nanosensor beads show selectivity towards detection of bilirubin in presence of interferences such as glucose, sucrose, metal ions, cholesterol, and biliverdin with limit of detection of 20 nM. Ultimately, the invention also provides a kit for visual detection of bilirubin in human serum.

The present disclosure is directed to a thin-film element that enables analytes to be analyzed on separate surfaces. In an example, a thin-film element includes a first layer for processing a fluid sample to generate a first analyte and a second analyte. The thin-film element also includes a second layer configured to be impermeable to the first analyte to enable the first analyte to be retained by the first layer and permeable to the second analyte to enable the second analyte to pass through the second layer. The thin-film element further includes a third layer configured to retain the second analyte. The second layer includes a first reflective surface and a second reflective surface to provide reflectance signals indicative of analytes present in the first and third layers to sensors located on opposite sides of the thin-film element.

Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.

The present invention relates to new methods for assessing the presence of nonalcoholic steatohepatitis (steatosis) in a patient, using functions combining biological markers without bilirubin and Body Mass Index being used as markers in the function.

Identifying and classifying a liver condition in a human subject based on a blood sample obtained from the human subject in a system being functionally associated with at least one analyzer for analyzing the blood sample is disclosed. The system includes at least one of an input interface or a transceiver, one or more processors, and a computer readable storage medium for instructions execution by the processor(s). The storage medium has stored instructions to receive biographical information relating to the human subject, and instructions to receive, from the analyzer(s), measurements of a plurality of serum biomarkers. The storage medium further has stored instructions to apply a neural network algorithm to the received measurements and biographical information, and instructions to identify, based on an output of the neural network algorithm, the presence of a liver condition, and to classify a severity of the liver condition.

An analytical toilet comprising a bowl for receiving excreta from a user, a urine collection chamber in fluid communication with the bowl, a sensor for detecting properties of the urine after at least partial settling of the urine, a valve adapted to release the captured urine from the urine collection chamber, and a source of flush water to clean the urine collection chamber.

An analytical toilet comprising a bowl for receiving excreta from a user, a urine collection chamber for receiving urine from the bowl, a sensor for detecting properties of the urine, a valve adapted to release the captured urine from the urine collection chamber, and a source of flush water to clean the urine collection chamber.

A testing pad comprising a water-resistant base layer, a water-transmissive top layer coupled with said base layer, an hydrophilic intermediate layer contained between said top layer and said base layer, and a color-changing chemical reagent contained between said top layer and said base layer adapted an configured to allow an animal or person to urinate on the testing pad and the chemical reagent to present testing results related to the presence, absence and/or concentration of substances within the urine.