Embodiments described herein include methods and assays for detecting beta-lactam analytes and mycotoxin analytes in a sample. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.

[Problem] To provide a method for rapidly evaluating the resistance of microorganisms such as bacteria to a drug.

[Solution] The method comprises an extraction step of performing an operation to obtain an extract containing a drug-degrading enzyme that can be produced by the microorganism from a sample containing the microorganism; a mixing step of mixing the extract and a drug to obtain a mixture; an analysis step of subjecting the mixture to mass spectrometry; and an evaluation step of determining whether the microorganism is resistant to the drug by detecting the presence or absence of a peak derived from a degradation product of the drug from the mass spectrum obtained in the analysis step.