An electrochemical device for diagnostic purposes, and particularly point-of-care diagnostic purposes, is capable of detecting quorum sensing molecules, such as AHL, within a biological sample with high precision. The device includes at least one reference electrode (RE), at least one counter electrode (CE), and two or more working electrodes (WEs). Each working electrode differs from the other working electrode(s) with respect to at least one of the following characteristics: surface area, size, material, and coating. The device also has a sample receiving area for receiving a biological sample, wherein the electrodes and the sample receiving area is fluidly connected, and a means for transferring the sample to the electrodes for measurement, and a means for displaying a result of the measurement.

Instruments, systems, and methods for measuring optical density of microbiological samples are provided. In particular, optical density instruments providing improved safety, efficiency, comfort, and convenience are provided. Such optical density instruments include a handheld portion and a base station. The optical density instruments may be used in systems and methods for measuring optical density of biological samples.

This disclosure relates to methods for determining membrane potential across membranes of organelles and cells. More particularly, this disclosure relates to nucleic add complexes comprising a voltage-sensing fluorophore conjugated to one single-stranded nucleic acid molecule and methods for using such nucleic acid complexes in determining the membrane potential.

Scintillant-doped polystyrene core nanoparticles surrounded by a silica shell can be used to quantify low-energy radionuclides. The nanoparticles are recoverable and re-useable, which may reduce waste and allow for sample recovery. Unlike traditional liquid scintillation cocktail (LSC) formulations, the nanoparticles are made from non-toxic and non-volatile components, and can be used without the aid of surfactants, making them a possible alternative to LSC for reducing the environmental impact of studies that employ radioactive tracers. Recognition elements attached to the functionalized silica surfaces of the nanoparticles allow for separation-free scintillation proximity assay (SPA) applications in aqueous samples. Lipid membrane coatings deposited on the nanoparticle surface can significantly reduce the non-specific adsorption of proteins and other biomolecules, and allow for the incorporation of membrane proteins or other membrane associated binding molecules.

In an approach, a biomedical device comprises at least one electrode, wherein the at least one electrode is coupled with a computer chip; at least two chemical sensors, wherein the at least two chemical sensors are coupled with the computer chip; the computer chip, wherein the computer chip comprises: a semiconductor substrate, and a processor; a microfluidic structure, wherein the microfluidic structure is an inert elastomeric polymer; a power supply device coupled to the computer chip; and an antenna configured to send data collected onto the computer chip to a remote server. In an approach, a processor stimulating a cell sample. A processor senses the presence of at least two types of biomolecules released by the cell sample. A processor records data collected by the at least two chemical sensors. A processor sends the recorded data to a remote server.

A cartridge comprises a chip chamber configured to house a microfluidic chip comprising a plurality of sets of cell channels configured to capture cells from a biological sample. The cartridge also comprises a sample chamber configured to receive the biological sample and be in fluid connection with the plurality of sets of cell channels and a plurality of medium reservoirs. Each medium reservoir of the plurality of medium reservoirs is configured to be in fluid connection with a respective set of cell channels of the plurality of sets of cell channels. The cartridge further comprises culture medium source in fluid connection with the plurality of medium reservoirs and configured to supply a culture medium to the plurality of medium reservoirs.

A method for evaluating neuronal functional connectivity can be used as a model for evaluating functional repair against damage to neuronal populations. The method can be used for multiple cell populations that are spaced apart from each other and connected via neurites, A method for evaluating neuronal functional connectivity in vitro involves cutting the neurites; observing the dynamics of neurons whose neurites have been cut; and measuring the electrical activity of each of the plurality of cell populations before and after the cutting. At least one of the cell populations contains neurons.

An apparatus is disclosed which uses electrodes to analyse a test sample.

In an approach, using a biomedical device, a processor stimulates a cell sample. A processor senses, based on feedback from at least two chemical sensors of the biomedical device, the presence of at least two types of biomolecules released by the cell sample. A processor records, using a computer chip of the device, data collected by the at least two chemical sensors. A processor sends, using an antenna of the biomedical device, the recorded data to a remote server.

A system and method utilize capacitance sensor data to identify cell events with single-cell resolution. The method identifies patterns in the sensor data related to events such as mitosis, migration-in to the sensor field, and migration-out. The system may include a processor co-located with the sensor to perform the pattern recognition. Further, microfluidic channels can be provided to direct cells to the sensors.