Disclosed are novel lanthanide(III) chelates including a pyridine 4-ethynylpyrazine subunit. These chelates have an excitation wavelength which allows excitation with UV LED.

Disclosed herein are oligomeric machines comprising a first oligomeric module having a first end and a second end, and a second oligomeric module having a first end and a second end; wherein the first end of the first oligomeric module is joined to the first end of the second oligomeric module; and wherein the oligomeric machine exhibits stochastic resonance and/or spontaneous vibrations in a solution at a temperature when the temperature is in a critical temperature range and the oligomeric machine does not exhibit stochastic resonance in the solution when the temperature is not in the critical temperature range; and the oligomeric machine exhibits stochastic resonance and/or spontaneous vibrations in a solution under a force load applied to the oligomeric machine when the force load is in a critical force range and the oligomeric machine does not exhibit stochastic resonance and/or spontaneous vibrations in the solution when the force load is not in the critical range. Also disclosed herein are molecular sensors comprising an oligomeric machine and configured to bind with one or more analytes thus modulating the stochastic resonance and/or spontaneous vibrations of the oligomeric machine. Additionally disclosed are uses of molecular sensors for the detection of one or more analytes in a solution.

Disclosed herein are oligomeric machines comprising a first oligomeric module having a first end and a second end, and a second oligomeric module having a first end and a second end; wherein the first end of the first oligomeric module is joined to the first end of the second oligomeric module; and wherein the oligomeric machine exhibits stochastic resonance and/or spontaneous vibrations in a solution at a temperature when the temperature is in a critical temperature range and the oligomeric machine does not exhibit stochastic resonance in the solution when the temperature is not in the critical temperature range; and the oligomeric machine exhibits stochastic resonance and/or spontaneous vibrations in a solution under a force load applied to the oligomeric machine when the force load is in a critical force range and the oligomeric machine does not exhibit stochastic resonance and/or spontaneous vibrations in the solution when the force load is not in the critical range. Also disclosed herein are molecular sensors comprising an oligomeric machine and configured to bind with one or more analytes thus modulating the stochastic resonance and/or spontaneous vibrations of the oligomeric machine. Additionally disclosed are uses of molecular sensors for the detection of one or more analytes in a solution.

The present invention provides methods of detecting analytes using particles having different physico-chemical properties, such as buoyancy, size, density, spectral characteristics, and/or binding properties, in solution-based sandwich assays and solution-based competition assays. The methods can be performed using rotors and bench-top centrifuges and provide for rapid, qualitative and quantitative detection of analytes. The present invention also provides kits that can be used to perform the methods, and mixtures containing particles suitable for the methods.

The present invention provides methods of detecting analytes using particles having different physico-chemical properties, such as buoyancy, size, density, spectral characteristics, and/or binding properties, in solution-based sandwich assays and solution-based competition assays. The methods can be performed using rotors and bench-top centrifuges and provide for rapid, qualitative and quantitative detection of analytes. The present invention also provides kits that can be used to perform the methods, and mixtures containing particles suitable for the methods.

A micromechanical sensor comprising a nucleic acid or nucleic acid analog nanostructure, such as a DNA origami or tiled structure, sensor spring and fluorophores arranged in FRET pairs in the sensor spring is provided, as well as methods of using the micromechanical sensor.

Provided herein is a method for biosensing a target substance [110] using a collection of particles [104] tethered to a surface [100] by tether molecules [102] and a plurality of capture molecules. A concentration of the target substance is determined from the time sequence of individual association/dissociation rates of the capture molecules. Competitive assay configurations are also described.

Provided herein is a method for biosensing a target substance [110] using a collection of particles [104] tethered to a surface [100] by tether molecules [102] and a plurality of capture molecules. A concentration of the target substance is determined from the time sequence of individual association/dissociation rates of the capture molecules. Competitive assay configurations are also described.

The invention relates to complexing agents of formula (I):

##STR00001##

wherein Chrom.sub.1, Chrom.sub.2 and Chrom.sub.3 are as defined in the description. The invention also relates to lanthanide complexes obtained from these complexing agents.

Disclosed herein include methods, compositions, and kits suitable for use in detecting the activation level of a signal transducer. In some embodiments, there are provided synthetic protein circuits wherein recruitment of synthetic protein circuit components to an association location upon activation of a signal transducer generates an active effector protein. The effector protein can be configured to carry out a variety of functions when in an active state, such as, for example, inducing cell death. Methods of treating a disease or disorder characterized by aberrant signaling are provided in some embodiments.