The present invention provides an improved capacitive bead sensor for detection and/or quantification of target analytes in a sample, with a detection limit down to single-beads, which is re-usable for multiple bead tests, or for a continuous flow of beads, and which is easily manufacturable and automatable. It enables sensitivity down to single molecule detection without the need for enzymatic amplification such as PCR, by use of various structural advantages and electronic signal amplification techniques that further allow for multiplex target detection not only across various nucleic acid targets but across entire target classes allowing for simultaneous detection of viral nucleic acids and host antibodies to that virus for example.

A method for measuring the presence of calprotectin (S100A8/A) heterodimer in a biological sample using a particle-enhanced turbidimetric immunoassay (PETIA) based on monoclonal antibodies. The method can be adapted on automated standard analyzers and provides a reliable clinical measurement of calprotectin in faecal samples and extracts. The method is comparable to commerical two-site sandwich ELISA. The disclosed method counters spontaneous agglutination caused by calcium ions and low-molecular weight calcium-binding S100 proteins as observed with conventional PETIAs. The method can be used for measuring the presence of human calprotectin in stool, urine, serum, plasma, synovial liquid and other body liquids. Metrological traceability and high commutability with conventional immunoassays (ELISA) has been shown despite of different measurement principles used.

Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

Beads for use as a control, calibration and/or quantification probe in a mass cytometry assay, wherein the beads are labeled with a heavy metal selected from the group comprising osmium or ruthenium. Also disclosed are beads labeled with a heavy metal exhibiting a surface functionalization that allows for the binding of an affinity reagent, such as a metal-conjugated antibody. Methods are described for the labeling of the beads and usage of the beads for quantification of cell surface receptors or for a compensation of channel crosstalk in mass cytometry assays.

The invention relates to a method, to a surface, to a particle, and to a kit for the detection of low molecular weight analytes such as crop protection agents in samples. In particular, the invention relates to a method for the detection of glyphosate through protein-functionalised surfaces and functionalised particles by means of reflection interference contrast microscopy (RICM).

Provided is a method of detecting the presence of an anti-Zika virus (ZIKV) antibody in a sample, including contacting a sample with a suspension having a plurality of microspheres wherein individual microspheres are conjugated to a peptide and the peptide includes a ZIKV peptide selected from the group including ZIKV NS1, ZIKV NS5, and ZIKV envelope protein, forming a first incubated suspension by incubating said sample with said suspension to permit binding of anti-ZIKV antibodies present in the sample to said microspheres, forming a second incubated suspension by contacting said first incubated suspension with an anti-ZIKV antibody detecting-reagent to permit binding of the anti-ZIKV antibody detecting reagent to said microspheres, removing from the second incubated suspension anti-ZIKV antibody detecting-reagent molecules that are not bound to said microspheres, and detecting the presence of anti-ZIKV antibody detecting-reagent molecules in the second incubated suspension. Also provided is a kit containing reagents and compositions for performing the foregoing method.

The present invention relates to the use of a particle, including a virus-like particle (VLP), for the discovery and analysis of protein-protein interactions that are modulated by small molecules.

This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

The present disclosure provides methods for assaying antibodies and related compositions, systems, and kits. More specifically, the disclosure relates to double-multiplex assays that detect multiple immunoglobulin isotypes against multiple different antigens simultaneously. The double-multiplex assay may be conducted using a single test sample.

The present invention is directed immunoassay methods, which re-use a hapten-immobilized test probe and reagents for quantitating an analyte in different samples, anywhere from about 3 to 20 times, while maintaining acceptable clinical assay performance. The methods use a dual antibody conjugate solution comprising an anti-hapten antibody and a capture antibody against the analyte in each cycle. After the completion of each cycle of reaction, the test probe is dipped in an acidic solution having pH about 1-4, to elute the immunocomplex formed on the probe and to regenerate the hapten-immobilized probe. The robustness of the hapten-coated solid phase allows utilization of multiple denaturation reagents for efficient elution of the immune complexes after each cycle without compromising the binding activity of the hapten on the solid phase.