The present disclosure provides a particle showing a small non-specific adsorption, having a reactive functional group for chemically bonding a ligand thereto, and being suitable for an agglutination method; a particle for the agglutination method having a ligand chemically bonded to the particle; a reagent, a kit and a method for detecting a target substance, each for in vitro diagnosis, each of which contains the particle. The particle includes a polymer having a unit having a linker A in a side chain, wherein when both terminals of the linker A are represented by X1 and X2, the linker A has a reactive functional group for chemically bonding a ligand thereto in one of X1 and X2; one of X1 and X2 includes an ester structure; and a sum of bonds between atoms linearly connecting X1 and X2 is 18 to 24 and the X1 and X2 contain CH.sub.2 or CH.

Methods and kits for accurately detecting one or more analytes in a sample by removing non-specific binding signals utilizing capture and control microparticles. The capture microparticles can specifically bind to the analyte while the control microparticles do not specifically bind to the analyte but to the background molecules. Both capture and control microparticles are added to the sample under suitable conditions to allow binding between analytes and the microparticles. Detection agent is then added to bind to analytes and other substances captured by the microparticles. The microparticles are then run through a cytometry-based detection method, where detection signals from the capture and the control microparticles are distinguished. The differences between the detection signals from the capture and the control microparticles are obtained, which are then used to determine the presence and/or amounts of the analytes based on a previously determined relationship between such differences and known amount of the analyte.

Embodiments described herein may be useful in the detection of analytes. The systems and methods may allow for a relatively simple and rapid way for detecting analytes such as chemical and/or biological analytes and may be useful in numerous applications including sensing, food manufacturing, medical diagnostics, performance materials, dynamic lenses, water monitoring, environmental monitoring, detection of proteins, detection of DNA, among other applications. For example, the systems and methods described herein may be used for determining the presence of a contaminant such as bacteria (e.g., detecting pathogenic bacteria in food and water samples which helps to prevent widespread infection, illness, and even death). Advantageously, the systems and methods described herein may not have the drawbacks in current detection technologies including, for example, relatively high costs, long enrichment steps and analysis times, and/or the need for extensive user training. Another advantageous feature provided by the systems and methods described herein includes fabrication in a relatively large scale. In some embodiments, the systems and methods may be used in conjunction with a detector including handheld detectors incorporated with, for example, smartphones (e.g., for the on-site detection of analytes such as pathogenic bacteria).

Disclosed are devices and methods capable of multiplexed analysis of multiple cellular activities and pathways in single cells including genomic, transcriptomic, and proteomic analysis.

Provided is a testing device including: a porous flow path member constituting a flow path through which a testing target liquid is flowed; a testing target liquid dropping portion provided on the flow path member; a labeling portion configured to apply a label to a target nucleic acid when the target nucleic acid is contained in the testing target liquid dropped onto the testing target liquid dropping portion; and a detecting portion configured to detect the target nucleic acid labeled at the labeling portion, wherein the testing device includes a shaped body formed of a resin on the flow path member at the detecting portion, and wherein a capture nucleic acid including a sequence bindable and complementary with the target nucleic acid is immobilized by covalent binding to a surface of the shaped body between the shaped body and the flow path member.

The invention relates to a micro-nano particles detection system and a method thereof. The system comprises a heating unit (1), a sample chamber unit (2), and a signal acquisition unit (4), wherein the heating unit (1) is arranged outside the sample chamber unit (2) for heating a sample in the sample chamber unit (2). Micro-nano particle fluid is loaded in the sample chamber unit (2). After the heating unit (1) heats the sample chamber unit (2), the sample chamber unit (2) generates a thermophoresis effect, so that the micro-nano particles are gathered at one side with temperature lower than the micro-nano particle fluid in the sample chamber unit (2). The signal acquisition unit (4) is used for collecting relevant information of the gathered micro-nano particles, and carrying out corresponding analysis.

Provided are compounds, compositions, kits, systems, devices, and methods for improving an assay such as, for example, a multiplexed PCR assay (e.g., a multiplexed immuno-PCR assay). A solid support (e.g., a bead) may be provided according to some embodiments of the present invention. The solid support may comprise an encoding agent (e.g., a dye), a nucleic acid sequence (e.g., an oligonucleotide and/or primer); and a molecular recognition element (e.g., an antibody). A detection reagent may be provided according to some embodiments of the present invention. The detection reagent may comprise a molecular recognition element (e.g., an antibody) and a nucleic acid tag. In some embodiments, at least a portion of the nucleic acid sequence of the solid support and at least a portion of the nucleic acid target tag of the detection reagent are configured to participate in a nucleic acid amplification process. A solid support and detection reagent may bind to the same target, thereby forming a reagent pair.

An object of the present invention is to provide a composite particle for an immunochromatography which has excellent spreadability, a method for manufacturing the same, and an immunochromatography using the composite particle for an immunochromatography. A composite particle for an immunochromatography of the present invention is a composite particle for an immunochromatography in which magnetic particles having an average particle size of 500 nm or less and gold particles having an average particle size of 500 nm or less are bound via an organic substance.

Flow cytometry of extracellular vesicle (EV) samples produces counts associated with channels defined by combinations of capture agents and detection agents, typically capture antibodies and detection antibodies having associated markers such as fluorophores. Sample groupings are obtained by processing channel counts using principal component analysis or other techniques. Identification of a particular sample grouping permits selection of associated channels for detection of samples exhibiting characteristics of the particular sample grouping.

Methods for separating microorganisms from non-microorganism cells in a non-microorganism cell-containing sample comprise incubating the sample with particles to form particle-microorganism complexes and then separating the particle-microorganism complexes from the non-microorganism cells. These methods are used to detect the absence or presence of a microorganism in a sample that also contains non-microorganism cells. Particular reagents and combinations of reagents enhance the selective capture of microorganisms in mixed samples. Corresponding compositions and kits are also provided.