Provided herein is a method of loading wells with a liquid droplet, or a portion thereof, wherein each liquid droplet comprises solid supports and a detergent or surfactant, such that the detergent or surfactant reduces the contact angle between the liquid droplet and the wells. Also provided is a method of detecting and quantifying an analyte of interest in a sample, which involves loading wells in an array with a liquid droplet according to aforementioned method, wherein the liquid droplet comprises an analyte captured on a solid support.

The present invention relates, in part, to methods of using X-ray fluorescence (XRF) spectrometry for analyzing (e.g., measuring) the binding of a soluble target to an insoluble material.

A method of acoustophoresis using selection particles that alter acoustic response is provided. The method can include selecting a set of selection particles based on surface markers of a plurality of target particles to be separated using acoustophoresis. The method can include incubating the set of selection particles with the plurality of target particles in a solution such that the set of selection particles bind with the surface markers on the plurality of target particles to create a plurality of bound particles. The method can include providing the plurality of bound particles to an acoustophoresis device tuned to separate the particles based on a net acoustic contrast between each of the plurality of bound particles. The method can include receiving a plurality of output streams from the acoustophoresis device that each include a respective bound particle of the plurality of bound particles.

A kit-of-parts for attaching an object on a substrate (1, 1′) having (i) at least a first solution having at least one first compound, wherein the first compound is at least one of a gelling agent, a gellable agent, and a thickening agent, and (ii) at least a first substrate (1, 1′) having a surface (2, 2′). The first solution is suitable for forming at least a first dispersion of an object in the first solution when a the object is added to the first solution. The first dispersion is suitable for attaching the object on a functionalized surface (3, 3′) of the substrate (1, 1′) when the first dispersion is added to the functionalized surface (3, 3′) of the substrate (1, 1′).

In an electrochemical lateral flow immunological test method, flow of a sample solution is controlled. As a result, the reaction time is short and quantitative measurements and electrical measurements can be performed with excellent sensitivity and high accuracy, and the invention provides a sensor employed in the method. Electrode portions, electrically conductive portions for transferring electric current from the electrode portions, and connecting portions connected to an electrical measuring instrument for measuring the electric current values are arranged on a supporting body including a resin sheet, pads and the like disposed by partial lamination on the supporting body. A sample solution flows over the plurality of pads, and electrochemical detection is performed by controlling the flow at the position of the electrode portions. Furthermore, the flow is controlled by a flow rate control pad, a flow passage portion fiber pad, and flow rate control protruding portions.

Described herein is a beads-free bioprocessor as an automated and cost-effective T cell processing and manufacturing platform. T cells are a core component in CAR T cell therapies for cancer treatment, but are difficult to manufacture to scale in clinically relevant quantities. The 3D bioprocessor provides an alternative device that is scalable, beads-free, easy-to-use, and cost-effective for using CAR T cell therapy in cancer immunotherapy. Besides CAR T cell application, this platform technology has potential for many other applications such as cancer cell isolation.

An object of the present invention is to provide a kit and a method capable of achieving high-precision measurement of a measurement target substance in a biological sample in a wide concentration range from a low concentration to a high concentration by sufficiently avoiding the influence of antibodies such as anti-serum albumin antibody present in blood. According to the present invention, there is provided a kit for measuring a measurement target substance in a biological sample, the kit including: a labeled particle having a first binding substance capable of binding to the measurement target substance and having a first blocking agent; and a substrate having a second binding substance capable of binding to any one of the measurement target substance or the first binding substance and having a second blocking agent, in which the labeled particle is a luminescent labeled particle containing at least one kind of compound represented by Formula (1) and a particle, and the first blocking agent and the second blocking agent are different from each other. ##STR00001## Each symbol in Formula (1) has the meaning described in the present specification.

The invention provides an assay method for an assay system using an anti-immunocomplex antibody, which is able to increase reaction efficiency. The n immunoassay method uses: an anti-hapten rabbit monoclonal antibody immobilized on a water-insoluble carrier, and a labeled anti-immunocomplex antibody,
wherein the method comprises the following steps (i) to (iii): (i) a step of reacting the anti-hapten rabbit monoclonal antibody immobilized on the water-insoluble carrier with a hapten in a solution to be measured, (ii) a step of reacting the labeled anti-immunocomplex antibody with the hapten-anti-hapten rabbit monoclonal antibody immune complex, and (iii) a step of detecting the signal from the label.

The present invention is directed to methods for using particles (e.g, microparticulate, nanoparticulate; magnetic, non-magnetic) comprising surfaces comprising capture moieties as described herein, to remove an interference as described herein, or enrich biomarkers, especially antibodies, prior to a diagnostic test, or to be isolated and used for prophylactic or therapeutic purposes.

Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays for screening and detection of a variety of molecules.