The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.

The present invention relates to methods for the detection of an analyte, such as coronavirus (e.g. SARS-CoV-2) or other virus particles and proteins, in a test sample. The invention also provides a flow device for use in such methods. Additionally, there is provided a coronavirus-binding reagent having the structure [sialic acid]-[linker]-[polymer]-[gold nanoparticle] for use in the devices and methods of the invention.

Some examples described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.

This invention relates to an apparatus for conducting immunoassay test. The apparatus includes a groove unit having a groove along a vertical direction configured to hold a rod-shaped portion of a probe along the vertical direction, and a push pin configured to move along a horizontal direction, the push pin being capable of residing at a first position and a second position. A tip of the push pin is capable of pressing the rod-shaped portion of the probe against the groove when the push pin resides at the first position. The distance between the tip of the push pin and the groove is larger than a diameter of the rod-shaped portion of the probe when the push pin resides at the second position.

This application relates to assays, devices, and methods for conducting highly sensitive assays that employ two binding agents and are useful in detecting specific targets such as antigens. These devices and methods provide the ability to detect minute amounts of the specific target with reduced risk of false positive results.

The invention relates to methods for preparing a compound comprising a peptide attached to a molecular scaffold whereby multiple peptide loops are formed, to compounds that can be obtained with such methods and uses thereof.

A support for a multiplex binding experiment is functionalized with at least two different polypeptides. The polypeptides are provided in a reaction mixture along with their cognate binding partners. The polypeptides have high affinity for their cognate binding partners provided in the reaction mixture. The polypeptides and their cognate binding partners can be used in immunoassays.

An irreversible and covalent method for immobilizing a glycoprotein includes the following steps. An organic boronic acid and a photoaffinity reagent are provided to contact a surface of a solid support, where the organic boronic acid is represented by R.sub.1—ArB(OH).sub.2, —ArB(OH).sub.2 is a boronic acid group, and R.sub.1 is a first cross-linking agent. The organic boronic acid is bound to the surface through the first cross-linking agent, and the photoaffinity reagent is bound to the surface through a second cross-linking agent R.sub.2. Next, a glycoprotein is provided to contact the organic boronic acid, and the glycoprotein includes an Fc fragment. An alcohol group on a sugar chain of the Fc fragment and the boronic acid group of the organic boronic acid form an organic boronate ester to immobilize the glycoprotein. UV light irradiation is then performed, so that the photoaffinity reagent and the glycoprotein form a covalent cross-link.

Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.

A device, interface complex, diagnostic system, kit or method for use in binding analyte of interest, wherein immobilizing is on an aluminum oxide surface. An interface molecule is immobilized on the aluminum oxide surface. Attached to the interface molecule, is a cross linking agent for binding to the analyte, or a biomolecule specific to the analyte. The interface molecule includes a polypeptide having at least one carboxy rich domain providing at least 5 free carboxyl groups within a molecular volume of 2.2-25 nm.sup.3, the free carboxyl groups being provided by amino acids containing two or more carboxyl groups, through which the interface molecule is immobilized to the aluminum oxide surface. The biomolecule may be covalently attached to the interface molecule, or the biomolecule may bean engineered antibody attached to the interface molecule through an antigenic determinant or through an Fc fragment.