A device and a method of using the device for determining hematocrit in a whole blood sample. The device includes a first portion having an introducer, at least one fluid channel, a fluid actuator, and an analysis sensor and conductivity sensor disposed within the fluid channel. The second portion includes at least one well containing at least one material. The first portion and second portion are movable with respect to each other. The introducer is configured to transfer at least a portion of the material from the well in portion two into the fluid channel of portion one. The method includes measuring the resistance over substantially the entire portion of a whole blood sample and calculating an average hematocrit level of the whole blood sample based on the measured resistance.

Provided are affinity-based methods of isolating biological entities via a surface antigen from a sample with non-chromatographic and chromatographic methods being provided. Also provided is a dextran polymer, kits for use in the method of isolating a biological entity and an apparatus for performing the methods.

The present inventive concept relates to a method for measuring analyte concentration in a sample fluid, comprising: receiving dilution fluid or sample fluid comprising analyte in a microfluidic channel, wherein the dilution fluid or sample fluid further comprises a molecule which is different from the analyte; performing a first affinity-based assay in a first detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the dilution fluid or sample fluid; mixing the dilution fluid or sample fluid in the microfluidic channel with another of the dilution fluid or sample fluid to obtain a diluted sample fluid; performing a second affinity-based assay in a second detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the diluted sample fluid; performing a third assay in the second detection zone to measure a signal indicative of the concentration of the analyte in the diluted sample fluid; determining a concentration of the molecule in the received dilution fluid or sample fluid, based on the measured signal of the first affinity-based assay; determining a concentration of the molecule in the diluted sample fluid, based on the measured signal of the second affinity-based assay; and determining the analyte concentration in the sample fluid on basis of the measured signal indicative of the concentration of analyte in the diluted sample fluid and a ratio between the determined concentration of the molecule in the received dilution fluid or sample fluid and the determined concentration of the molecule in the diluted sample fluid. The present inventive concept further relates to a microfluidic arrangement for facilitating measurement of analyte concentration in a sample fluid, and to a system for measuring analyte concentration in a sample fluid, comprising the microfluidic arrangement, and to a diagnostic system comprising the microfluidic arrangement.

The invention concerns a method, an assembly and a system for determining a characteristic property of a molecular interaction. The method includes providing a liquid sample including a particle capable of being in a state of equilibrium and in a state of non-equilibrium. The particle includes a marker in at least one of its state of equilibrium and state of non-equilibrium. The method further includes bringing the particle in a state of non-equilibrium by subjecting the sample to a condition jump comprising a jump in temperature and/or pressure; reading out the marker as a function of time during at least a portion of a relaxation time for said particle, and determining said characteristic property of said molecular interaction.

A microfluidic device includes a channel through which a reaction solution flows. The channel passes through a reaction section having a plurality of temperature zones set at predetermined different temperatures. The channel includes, at least in the reaction section, a region where a cross-sectional area decreases in a feeding direction of the reaction solution.

A lateral flow immunoassay system is provided that includes a housing having a base portion forming a first chamber therein and a body portion formed with three openings in fluid communication with the first chamber, a vial containing a buffer agent, and a sample collector for introducing a sample fluid into the first chamber via the second opening. The vial is mounted to the housing such that a dispensing side extends into the first opening for dispensing the buffer agent therefrom into the first chamber. The housing allows the buffer agent and sample fluid to be mixed within a reaction well formed within the first chamber to form a test sample mixture. The body portion is configured to receive a receiving end of an elongated holder in the third opening and allow a test strip secured in the holder to be brought into communication with the text sample mixture.

A dry stick (180a, 180b, 180c) arranged to indicate at least one biomarker value of a milk sample of an animal (100) by a lateral flow test. The dry stick (180a, 180b, 180c) includes a sample pad (310) with a reagent, configured to indicate at least one biomarker value of a received milk sample of an animal (100) by changing colours when exposed for milk having the biomarker; a porous membrane (320), configured to create a capillary flow of the milk sample, from the sample pad (310) through the porous membrane (320); an absorbent pad (330) with an absorbent configured to absorb superfluous milk from the porous membrane (320); and a porous desiccant, configured to absorb moisture from environmental air.

A flow cell with a first section and a second section, and a gasket sealing between the first and second sections. A chamber is defined in the flow cell, having a perimeter with a narrower end and a rounded wider end. An inlet passage, outlet passage, and a sensor are arranged in fluid communication with the chamber. The inlet passage directs fluid into the chamber proximal its narrow end at an angle of between about 45° and 75° relative to the plane of gasket and the outlet passage directs fluid flow out of the wider end of the chamber at an angle between about 45° and 75° relative to the plane of gasket, the inlet passage and outlet passage being angled in opposite directions. The flow cell is useful for monitoring levels of chemicals in an industrial process stream, such as lactose levels in a dairy process stream.

The invention provides a determination method for determining the presence of a target microorganism in a patient from a sample of said patient's stools, the method being characterized in that it comprises the following operations: obtaining a sample of liquid stools of said patient or a liquid sample obtained from stools of said patient, referred to as the liquid sample; pretreating the liquid sample with activated carbon; and using immunochromatography to detect in the resulting pretreated liquid sample the possible presence of at least one antigen of the target microorganism so as to come to a conclusion about the presence or the absence of the target microorganism in said patient.

The invention also provides a device (1) for detecting an antigen of a target microorganism in the liquid sample (3) by immunochromatography, the device including a zone (20) for purification with activated carbon (21).

Sensitive and specific detection of nucleic acids can be achieved using a chemical ligation-based template assisted rapid assay (TARA-L) with simple chemical reactions between probes and without the need for enzymes. Probes are designed to form a ligation product when they anneal to adjacent portions of a target nucleic acid. The ligation products can be detected, such as in immunochromatographic assays. The methods allow for the fast, efficient analysis of biological samples for the presence of nucleic acids and can be used, for example, in point of care settings.