The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.

The present disclosure provides methods and systems for performing single-molecule detection using fabricated integrated on-chip devices. In an aspect, the present disclosure provides a method for on-chip detection of an array of biological, chemical, or physical entities, comprising: (a) providing an array of light sensing devices; (b) immobilizing the array of biological, chemical, or physical entities on a substrate of the array of light sensing devices; (c) exposing the array of biological, chemical, or physical entities to electromagnetic radiation sufficient to excite the array of biological, chemical, or physical entities, thereby producing an emission signal of the array of biological, chemical, or physical entities; (d) using the array of light sensing devices, acquiring pixel information of the emission signal of the array of biological, chemical, or physical entities without scanning the array of light sensing devices across the array of biological, chemical, or physical entities; and (d) detecting the array of biological, chemical, or physical entities based at least in part on the acquired pixel information.

The present invention provides the methods of detecting the cleavage activity of PCs and proteases that are in purified form or from a biological sample and target to SARS-CoV-2 spike protein cleavage.

Provided herein are methods of determining a location of a biological analyte in a biological sample.

A sensor uses a combination of biocompatible quantum dots and an organic fluorophore in a controlled ratio. The organic fluorophore exhibits fluorescence of a first color, and the biocompatible quantum dots are sized to exhibit fluorescence of a second color different from the first color. The biocompatible quantum dots are functionalized with an organic coating arranged to chemically interact with a substance to quench the fluorescence of the quantum dots. The sensor exhibits a ratio of fluorescence of the quantum dots and the organic fluorophore from which a presence of the substance can be detected.

The present disclosure relates to methods and compositions, e.g., kits, for assessing or detecting SARS-CoV-2 antigen(s), e.g., SARS-CoV-2 nucleocapsid protein (N-protein), in a sample or a blood sample, e.g., serum, plasma, dried blood spots (DBS) and/or a saliva sample. Certain applications and uses of the present methods and compositions, e.g., kits, are also provided.

A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

The present invention relates to a microfluidic device (2) comprising a support part (21) and a cover part (22) defining together a microchannel (5), said microchannel (5) having a surface, said surface comprising:—a first area (9) which is grafted with a first ligand, and—at least a second area (10) which is distinct from the first area (9) and which is grafted with a second ligand which is different from the first ligand, wherein each ligand is capable of binding to a target, the targets being different from each other. The present invention further relates to a microfluidic detection system comprising the said microfiuidic device, a reservoir adapted for containing a sample to be analysed and a detection device for detecting and quantifying the targets. The present invention relates also to a method for manufacturing such a microfluidic device, as well as a method for analysing a sample containing targets using the said microfluidic device or microfluidic detection system.

Systems, methods, and devices for analyzing small volumes of fluidic samples, as a non-limiting example, less than twenty microliters are provided. The devices are configured to make a first sample reading, for example, measure an energy property of the fluid sample, for example, osmolality, make a second sample reading, for example, detecting the presence or concentration of one or more analytes in the fluid sample, or make both the first sample reading and the second sample reading, for example, measuring the energy property of the fluid sample as well as detecting the presence or concentration of one or more analytes in the fluid sample.

The assay device (10) comprises a micro flow path (76); a porous medium provided near the distal end portion (80) of the micro flow path (76); and a space (82) provided between the micro flow path (76) and the porous medium for controlling the flow rate of a fluid moving from the micro flow path (76) to the space (82). After a fluid moved along the micro flow path (76) based on a lateral flow is brought into contact with the porous medium beyond the space (82) and is absorbed to the porous medium, the fluid is divided by the space (82) so that the fluid stays in the micro flow path (76). With this structure, it is possible to perform solution exchange in the micro flow path without using an external device such as a pump.