The invention is a novel and non-obvious design and implementation of an inductive sensor for quantifying magnetic particles. The invention parts way from the conventional methods of using wounded coils to a design that is compatible with an integrated circuit (IC) chip fabrication processes and/or printed circuit board (PCB) manufacturing. The increased accuracy from these fabrication methods provides a significant improvement to sensor sensitivity. In addition, the design of the inductive sensor enables easy integration with lateral flow assay (LFA) technology. The sensor can be applied to detect and quantify molecules to provide information on health, hazard or safety.

The present invention provides a detection device of dot immunoblotting detection, including a negative pressure suction device, a hole plate, a hose for connecting the hole plate with the negative pressure suction device, and a nitrocellulose membrane in tight fit with the upper end surface of the hole plate. The present invention further provides a detection method of dot immunoblotting detection, including preparation, sample injection, blocking, incubation of a primary antibody, incubation of a secondary antibody, development and analysis. The detection method performs negative pressure suction through the negative pressure suction device, which is favorable for concentrating samples during sample injection and avoiding the influence, caused by cross contamination after diffusion of the samples, on an experimental result, and the experimental result is more accurate.

Disclosed are a magnetic particle luminescent micro-fluidic chip for multi-marker detection and a detection apparatus. The chip includes a top plate, provided with at least one sample loading part communicated with a mixing area, and a plurality of labeling ligands arranged in the mixing area; a bottom plate, including a flow guide area communicated with the mixing area, reaction areas communicated with the flow guide area, a plurality of detection areas communicated with the reaction areas, a cleaning liquid storage part and a luminescent liquid storage part which are communicated with the detection areas; and an air pump, disposed on the top plate and configured to drive a sample in the sample loading part to flow through the mixing area.

Disclosed are a magnetic particle luminescent micro-fluidic chip for multi-marker detection and a detection apparatus. The chip includes a top plate, provided with a sample loading part and a mixing area with a plurality of labeling ligands; a bottom plate, including a flow guide area, a plurality of reaction areas communicated with a plurality of detection areas, a cleaning liquid storage part and a luminescent liquid storage part which are communicated with the detection areas, where each reaction area is provided with a different magnetic particle ligand; a cleaning liquid is stored in the cleaning liquid storage part, and a luminescent liquid is stored in the luminescent liquid storage part; and an air pump, disposed on the top plate and configured to drive a sample in the sample loading part to flow through the mixing area.

Provided are a reaction well cartridge device and reaction well cartridge system suitable for biochemical or biological reaction, the reaction well cartridge device including a cartridge body including at least one of a reaction well container containing a reaction well where biological reactions occur, pipette tip containers containing at least pipette tips, a sample container containing a sample, reagent containers containing reagents, a mixed solution container containing a mixed solution of the sample and the reagents, a diluent container containing a diluent, washing solution containers containing washing solutions, and combinations thereof, and a temporary storage provided on the cartridge body to temporarily store wastes produced after the reactions occur in the reaction well.

Methods and devices for ultrasensitive detection of target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices enable ultrasensitive determination of the concentration of target molecules.

A nanowire molecular sensor, and a molecular detection system, comprising a nanowire waveguide (30), a nanowire sidewall (51) functionalized in order to attach a molecule (54), and light emissive point sources (52), wherein the amount of light emitted at an end (53) of the waveguide is dependent of the amount of specific molecules attached to the sidewall of the nanowire. A method employing said sensor may be used for single cell detection and analysis.

Disclosed herein include systems, methods, compositions, and kits for measuring the secretion level of a secreted factor of a single cell. Disclosed herein include solid supports comprising a plurality of capture probes capable of specifically binding to secreted factors secreted by a single cell. In some of the embodiments, at least two of the capture probes are capable of binding different secreted factors. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe. Secreted factor-binding reagents can comprise a detectable moiety, or a precursor thereof. Secreted factor-binding reagents capable of binding the same secreted factor comprise the same detectable moiety, or a precursor thereof, and secreted factor-binding reagents capable of binding different secreted factors can comprise different detectable moieties, or precursors thereof.

The present invention relates to a test system or an assay system (detection system) and test method preferably for use in the Point-of-Care (PoC) field.

A sensor system detects a presence or concentration of an analyte in a medium. The sensor system contains a sensor having a sensor head with a chamber. The sensor head has a permeable area through which the analyte can pass into the chamber when the sensor head contacts the medium. A cross-linked hydrogel fills the chamber, the hydrogel is configured to undergo a change in volume when contacting the analyte passed into the chamber which leads to a change in pressure in the chamber. A pressure sensor is configured to measure the pressure in the chamber for detecting the presence or concentration of the analyte.