The present invention discloses a kit and a novel method of extraction of a progesterone metabolite from feces/dung sample. The present invention also discloses a paper-based microfluidic device for detection of the progesterone metabolite for early determination of pregnancy in cattle and buffaloes. The invention further discloses antibodies developed against said metabolite, method of extraction, methods for fabricating the paper-based microfluidic devices and methods for inexpensive, rapid and non-invasive determination of pregnancy in early stages in cattle and buffaloes.

Methods of surface modification of silicones for specific target and high efficiency binding are disclosed. Namely, surface-attached microposts and methods of functionalizing the surface-attached microposts for target-specific analyte capture are provided. For example, a microposts processing platform is provided that is based on a microfluidic flow cell structure that includes a reaction (or assay) chamber. The method utilizes the microposts processing platform that includes an arrangement of surface-attached microposts on at least one surface of the reaction (or assay) chamber. Methods of functionalizing the surface-attached microposts include one or more steps, wherein the incorporation of one or more functionalizing agents are used to provide a micropost surface for target-specific analyte capture. In one example, the micropost surface-functionalization process includes providing a binding system, wherein the binding system includes a generic binding agent and a linking agent for binding of a target-specific capture agent.

Microfluidic devices, method for making and using same are disclosed. The microfluidic device can be made by applying a reagent in liquid form into a sample treatment site within a substrate base. The substrate base has hydrophilic properties bordering a flow channel, and has an application site, the sample treatment site and an analytical site. The flow channel is sized and configured so as to draw a sample applied into the application site to the analytical site through the sample treatment site by capillary action. The reagent is freeze-dried within the sample treatment site.

An embodiment of an assembly for isolating a substrate is described that comprises a vacuum source; a substrate; a receptacle configured to position the substrate and to operatively couple to the vacuum source; and a vessel configured to operatively coupled to the receptacle, wherein the substrate is configured to move from the receptacle to the vessel in response to a differential pressure applied by the vacuum source.

A fluidic device includes a fluidic layer, a capture material, and an electronics layer, the fluidic layer includes a main channel and a pair of sample channels fluidly coupled to the main channel. The pair of sample channels is configured to receive and introduce a sample material into the device. The sample material includes an analyte. The capture material is positioned in a portion of the main channel that is spaced from the pair of sample channels. The capture material has a three-dimensional matrix of receptors therein configured to bond with the analyte. The capture material has a length that is associated with a dynamic range of the fluidic device and a cross-sectional area that is associated with a sensitivity of the fluidic device. The electronics layer includes electrodes configured to measure an electrical resistance through a portion of the capture material.

The present invention relates to controls which may be used to secure the results of multiplex analysis methods. The present invention thus relates to solid supports comprising one or several controls and their use in multiplex analysis methods to detect several analytes potentially present in a sample.

The present invention relates to a method for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor, comprising the steps of providing (101) a plurality of fluid samples, each containing known concentrations of analyte providing (102) a plurality of needles and a plurality of sensor surfaces or detection spots, at least some of the sensor surfaces or detection spots having a known amount of ligand immobilized thereon, and each needle being configured to inject a fluid sample to a sensor surface or detection spots dividing (103) said plurality of fluid samples into at least two groups injecting (104) the fluid samples of a first of said groups to the sensor surfaces or detection spots by means of the needles to permit association of the analyte to the ligand monitoring (104) each sensor surface or detection spot and collecting binding data, and repeating (105) the steps of injecting fluid samples and monitoring detection spots and collecting binding data for each group of fluid samples, wherein the steps above are performed sequentially, without intermediate regeneration or renewal of the immobilized ligand.

The invention also relates to a biosensor system for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface, to software for performing the steps of the method and to a computer readable medium for storing said software.

A method functionalizes a surface, wherein a polyphenol layer is formed on the surface by applying a polyphenol compound, and the surface is subsequently functionalized by binding a functional molecule to the polyphenol layer. In addition, the polyphenol layer is treated with an oxidizing agent before, during, or after binding of the functional molecule. An associated article can be prepared and used. A kit can be used for performing the method or producing the article.

The present invention provides devices, systems, and methods, for performing biological and chemical assays.

Systems are described, based on a primary binding compound and a secondary binding compound used in combination with a support to detect a target in a sample. The systems includes at least one support structure, at least one small primary support portion containing at least one molecule covalently bound to a visual colloidal marker, a plurality of secondary support portions comprising secondary binding compounds that are covalently bound to the support portions and chemically active, at least one pH litmus indicator, at least one pH strip, a buffer for lateral flow on the porous membrane support that allows preservation and activity of binding compounds.