Embodiments of the present disclosure are directed to systems, devices, and methods for the selective collection of cells from a heterogeneous cell population, including highly multiplexed detection of secreted and intracellular macromolecules and the targeted laser-assisted ablation of cells identified to be positive or negative for a given biomarker or phenotype. The resulting non-ablated cells can be collected individually or pooled to form a homogenous cell population for further processing including safe and efficacious cellular therapies.

Methods and systems for high-throughput Identification of receptor:ligand interactions are provided. Throughout this application various publications are referred to in parentheses. Full citations for these references may be found at the end of the specification. The disclosures of these publications, and all patents, patent application publications and books referred to herein, are hereby incorporated by reference in their entirety into the subject application to more fully describe the art to which the subject invention pertains.

The present invention relates to controls which may be used to secure the results of multiplex analysis methods. The present invention thus relates to solid supports comprising one or several controls and their use in multiplex analysis methods to detect several analytes potentially present in a sample.

A mobile phone-based system for ELISA (MELISA). The MELISA system can perform all steps of ELISA procedure and output hormone concentration values. The system includes a heater that keeps the temperature required for sample incubation, illumination system for sample image capturing, and a digital camera system. All components are enclosed in a dark box to create optimal light conditions for image capturing. After sample images are captured, they are digitally processed, and hormone concentration values are reported based on colorimetric data from the samples. The system can be used to complete all steps of the assay, including incubation and reading. It is lightweight, can be fabricated at low cost, is portable, and can transfer test results via mobile phone. MELISA can be calibrated for accurate measurements of progesterone and has demonstrated successful measurements with the calibrated system.

The present invention relates to a carbene compound having substituted at the terminal polyethylene glycol (PEG) having nitrogen-containing functional groups, a carbene-metal nanoparticle complex in which the carbene compound and metal nanoparticles are bounded, a preparation method thereof, and a biosensor using same.

The present disclosure provides devices, methods, and systems for performing cellular, biological, or chemical assays where a sample can have a plurality of analytes.

An embodiment provides a method for identifying Covid-19 in a body fluid of a patient, including: removing the body fluid from a patient; applying a treatment to the body fluid, wherein the treatment comprises an antibody that joins with a Covid-19 targeted antigen (TA) in the body fluid to form an antibody-TA complex, wherein the antibody comprises a light functional antibody; identifying the antibody-TA complex, using a spectroscopic technique, from the body fluid using a light source; and returning the body fluid to the patient. Other aspects are described and claimed.

Lateral flow devices, methods and kits for performing lateral flow assays are provided.

The present disclosure provides, in part, a microfluidic apparatus for detecting target molecules. More specifically, the present disclosure relates to a protein microarray-integrated microfluidic system for detecting target molecules.

A compact optical imaging system including a single filter and a light source that provides lateral illumination for bead detection in digital assays. The light source is configured to emit light toward the detection vessel. The single filter is positioned to receive light reflected from a sample in the detection vessel, that originated from the light source, and receive an output from a sample in the detection vessel. A detector is configured to receive a portion of the reflected light and a portion of the output that passes through the single filter.