The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

This disclosure relates to compositions and methods for analyzing single cells using cell printing and spatial analysis.

Disclosed is a carboxylic acid-functionalized 3-dimensional SERS substrate. A carboxylic acid-functionalized 3-dimensional SERS substrate according to an embodiment of the present invention includes a substrate; a 3-dimensional nanostructure including a multistacked metal nanowire array formed by alternately and repeatedly transfer printing a single-layer metal nanowire array, laminated on a polymer mold on which a pattern of a master mold is duplicated, onto the substrate 110 to be perpendicular to each other; and a functionalized carboxylic acid into which a residue of the polymer mold present on the 3-dimensional nanostructure is functionalized and which enables a target analyte to immobilize.

This disclosure relates to methods of preparing re-usable and dry-able cartridges comprising matrices comprising a detection molecule such as an antibody, for detection or enrichment of an analyte in a sample, such as an antigen recognized by an antibody. In some embodiments, the cartridge comprises a tube or well that holds the matrix. In some embodiments, the matrix or associated cartridges may be stored wet or dry. The disclosure also relates to the cartridges and methods of their use.

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

The present invention relates to a method for detecting a target analyte using a gold nanoparticle, comprising growing a copper crystal specifically on a gold nanoparticle by treating the gold nanoparticle with a solution comprising a copper ion, a polymer having a primary or secondary amine group, and a reducing agent, a composition for amplifying a signal used in the detection method above, and a kit for detecting a target analyte comprising the composition for amplifying a signal above.

The present invention provides an apparatus for detecting an analyte in a fluid sample, comprising a fluid sample collection element, comprising a fluid sample collection element that comprises an absorbing element; a carrier for accommodating a testing element; and a chamber for accommodating a testing element carrier, wherein the testing element carrier is located in the chamber, and the carrier further comprises an extrusion structure that is in contact with the absorbing element and extrudes the absorbing element.

In an embodiment, the present disclosure pertains to a method of detecting an analyte from vesicles in a sample. In an additional embodiment, the present disclosure pertains to an analyte detection platform. In a further embodiment, the present disclosure pertains to a sensor. In another embodiment, the present disclosure pertains to a method of detecting an analyte from a sample. In an additional embodiment, the present disclosure pertains to a method of lysing vesicles. In a further embodiment, the present disclosure pertains to a vesicle lysis platform.

The present disclosure provides methods and systems for detecting a presence or absence of analytes. The systems may comprise membrane-based substrates.

An optical biomodule for detecting a disease specific biomarker, utilizing an enhanced light emission due to integration of one or more three-dimensional (3-D) protruded structures in a fluidic container (which includes one or more materials), upon chemical binding of a disease specific biomarker binder (which includes a first segment coupled with a first fluorophore and a second segment coupled with a second fluorophore) with its corresponding disease specific biomarker is disclosed. Furthermore, the disease specific biomarker binder may include a synthetic biological logic circuit/gate. Furthermore, the three-dimensional (3-D) protruded structure can be coupled with a photonic crystal or a metamaterial (hyperbolic metamaterial/metamaterial of Epsilon-Near-Zero (ENZ)) of a suitable wavelength range.