Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.

A system for testing a treatment agent for a biologic material includes an input for receiving a biologic sample. A plurality of micro-pumps pump a portion of the biologic sample from the first reservoir into a connected module. A first module includes a first plurality of testing pathways for testing a first portion of the biologic sample. A first module connector removeably connects the first module to the distributor module. A second module includes a second plurality of testing pathways for testing a second portion of the biologic sample. The selected pathway applies at least one dosage level of a treatment agent to the second portion of the biologic sample. A second module connector removeably connects the second module to the distributor module, wherein treatment agent and the plurality of dosage levels tested by the system may be selected by selecting the second module associated with the second module connector.

An inspecting instrument to be used for measuring, using a test substance-containing solution containing a test substance and a liquid, which is contained in the test substance-containing liquid. The inspecting instrument includes a wall that has a periodic structure resulting from a plurality of recesses or protrusions, the plurality of recesses or the plurality of protrusions including a refractive index adjusting layer on surfaces thereof, the refractive index adjusting layer being a layer having a refractive index greater than a refractive index of the test substance-containing solution or being a silicon layer. A method of measuring the concentration of a test substance in a liquid, measured using the inspecting instrument, has high accuracy.

Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.

Herein are innovations that enable facile cryo-EM analysis of diverse samples. Methods of functionalizing sample grids for cryo-EM are described, including methods of creating high quality graphene oxide films on cryo-EM substrates. The cryo-EM sample substrates are functionalized with affinity molecules that efficiently concentrate sample molecules and other specimen types on the grid, away from the air-water interface. Affinity groups include amines and proteins such as tagging system proteins and peptides that can be used to capture diverse sample types with high affinity. Optionally, spacers such as PEG chains are used to place sample particles away from the substrate surface, reducing substrate-induced artifacts.

The present invention is drawn to devices and systems for allergen detection in food samples. The allergen detection system includes a disposable analysis cartridge and a detection device with an optimized optical system.

A method and a portable diagnostic apparatus (20) for detecting at least one analyte from a sample using a microfluidic cartridge (22). The portable diagnostic apparatus (20) comprises a cartridge receiving unit, a cartridge driver unit (30) and an optical unit (32). A method and an apparatus of obtaining disease prevalence information comprising at least one of the portable diagnostic apparatus (20). A method and a system for managing a network of portable diagnostic apparatuses and obtaining disease prevalence information comprising at least one of the portable diagnostic apparatus (20). A diagnostic system with multiple automated features that is capable of providing a one-step solution to near-patient clinical evaluation and diagnosis.

The present invention provides methods and devices for detecting and quantifying multiple biomolecules at single-molecule level using an integrated droplet microfluidic system. In one embodiment, the present invention provides real-time and digital measurement of multiple biomolecules in a sample, thereby quantifying multiple biomolecules in an absolute and simultaneous manner. In one embodiment, the present invention provides a diagnostic method for a disease, comprising real-time and digital measurement of multiple biomolecules in a sample using the method or device described herein.

Provided are devices, systems and methods for determining whether a patient is immune to an infection or a disease caused by a coronavirus, such as severe acute respiratory coronavirus 2 (SARS-CoV-2). Devices and systems described herein are cost effective, scalable, and may be used at the point of need or point of care without a specialized training. The systems and devices described herein are useful for vaccine development, screening convalescent plasma therapies, and for identifying individuals who are eligible for reintegration following a period of quarantine.

Described herein are systems and methods for detecting and quantifying analytes. Also described herein are systems and methods for detecting and quantifying analytes stemmed from infections.