Assay devices, systems, methods, and kits useful for detection of analytes in a bodily fluid sample, such as saliva, are disclosed herein. Assay devices may include a fluid sample collector and an assessment unit to which the fluid sample collector couples. The fluid sample collector may include a cap with a plunger and absorbent member that collects a bodily fluid sample. Insertion of the plunger into the assessment unit during coupling provides pressure-driven fluid flow through a filter rapidly delivering bodily fluid filtrate to one or more test panels which provide the assay result. In embodiments, an on-board light source enables a user to readily detect changes in a test panel indicative of the presence of an analyte thus providing an opportunity for obtaining rapid results with improved confidence.

A type of microfluidic platform, photothermal bar-chart chip (PT-Chip), uses on-chip nanomaterial-mediated photothermal effect as a tunable microfluidic driving force to drive ink bar-charts in a visual quantitative readout fashion. The photothermal bar-chart pumping performance can be adjusted remotely by tuning the irradiation parameters, without the need to change any on-chip parameters. The PT-Chip enables a POC visual quantitative diagnostics, by forming nanomaterial-mediated photothermal effects-driven bar-chart microchip for visual quantitative immuno-sensing. In this immunoassay, biomolecules are visually quantified by directly reading the distance that fluids move on the PT-Chip.

An assay cartridge for sensing multiple analytes includes a capture platform and main body. The capture platform includes appendages. The main body includes first, second, and third assemblies of reaction compartments as well as first and second wash basins. The main body includes a capture platform cradle that supports the capture platform. Each appendage is coupled to a capture agent that specifically binds to an analyte. The first reaction compartments each comprise a matrix that includes the analyte. The first wash basin includes a first wash solution that removes non-specific binding between the analyte and capture agent. The second reaction compartments each include a detection antibody. The second wash basin includes a second wash solution that remove non-specific binding of the detection antibody to the analyte. The third reaction compartments each include a substrate that reacts with a conjugated enzyme of the detection antibody and changes color as a result.

Brevetoxin (BTX) concentration levels in organisms and other samples are detected using conjugated recombinant monoclonal brevetoxin antibodies (BTX-rAbs) that are cross-reactive with one or more brevetoxin antigens. The analysis may employ a simplified and less pure, but more easily, more safely or more rapidly prepared crude tissue extract. The results may be used to permit or not permit the harvesting, sale, relocation or depuration of such organisms or samples.

Sensors having an advantageous design and methods for fabricating such sensors are generally provided. Some sensors described herein comprise pairs of electrodes having radial symmetry, pairs of nested electrodes, and/or nanowires. Some embodiments relate to fabricating electrodes by methods in which nanowires are deposited from a fluid contacted with a substrate in a manner such that it evaporates and is replenished.

Sensors having an advantageous design and methods for fabricating such sensors are generally provided. Some sensors described herein comprise pairs of electrodes having radial symmetry, pairs of nested electrodes, and/or nanowires. Some embodiments relate to fabricating electrodes by methods in which nanowires are deposited from a fluid contacted with a substrate in a manner such that it evaporates and is replenished.

The invention includes a method of assaying an analyte in a sample in a portable, handheld microfluidic reader. The method includes the steps of: inserting the sample in the reader; capturing the analyte with a first antibody having a DNA tag attached thereto; capturing the analyte in the sample with a second antibody attached to a surface or having a magnetic nanoparticle (MNP) attached thereto; where a sandwich including the magnetic nanoparticle, first and second antibodies, the analyte and the DNA tag is formed; replicating the DNA tag using isothermal amplification to a predetermined amount of DNA tags detectable by a detector sufficient to overcome the minimal mass sensitivity limitations of the detector; and measuring the amount of replicated DNA tags using the detector. The invention also includes an apparatus or handheld portable field microfluidic reader in which the method is performed.

A point-of-birth system and instrument, biochemical cartridge, and methods for newborn screening is disclosed. Namely, a point-of-birth system is provided that includes a point-of-birth instrument for receiving and processing a biochemical cartridge for performing newborn screening. Further, a portable smart device, such as a smartphone or tablet, is in communication with point-of-birth instrument, wherein the smart device may include a newborn screening (NBS) mobile app. In one example, the point-of-birth system and point-of-birth instrument support newborn biological screening only. However, in another example, the point-of-birth system and point-of-birth instrument support both newborn biological screening and newborn physiological screening.

The invention relates generally to in vivo collection of circulating molecules, tumor cells and other biological markers using a collecting probe. The probe is configured for placement within a living organism for an extended period of time to provide sufficient yield of biological marker for analysis.

The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.