A membrane carrier for a liquid sample test kit that detects a substance to be detected in a liquid sample, the membrane carrier including at least one integrally-molded flow path capable of transporting the liquid sample, in which a microstructure that causes a capillary action for transporting the liquid sample is provided on a bottom surface of the flow path, and the microstructure has two or more peak positions at which a height becomes maximum per one repeating unit structure.

The present specification provides methods to prepare reagents and microparticulate binding surfaces with specificity to anti-streptavidin interference and anti-biotin interference. Also disclosed is how to use reagents and microparticulate binding surfaces to block, deplete or reduce the concentration of anti-streptavidin interference and anti-biotin interference in samples below the assay blocking threshold (ABT) prior to a diagnostic test.

In one embodiment, the present invention includes a system for detecting a target analyte which includes a microfluidic device having least one microfluidic channel with a binding surface positioned in the microfluidic channel with further include a first electrode and a second electrode. The system may further include a detector and a voltage supply. Also included is a method to detect a target analyte using a described microfluidics device, introducing solution with a target analyte to a binding surface, and binding the target analyte to the binding surface by applying an electrical potential between the first and second electrodes during at least a portion of the binding step, which enhances the rate of binding of the target analyte molecules to the binding molecules. The method then includes the steps of detecting a reporter molecule which corresponds to the amount of the bound target analyte molecules, which correlates with the amount of target analyte in the original sample. The method may also include multiple applications of sample to the binding surface prior to the detection step.

In one cross-section, a protrusion (8) has a first region (RG1). An outer edge of the first region (RG1) of the protrusion (8) has a concave portion (R). The concave portion (R) is recessed in an X direction by a distance D with respect to a virtual straight line (IL). The virtual straight line (IL) extends in a Y direction. Specifically, the virtual straight line (IL) is in contact with a first position (P1) of an outer edge of the protrusion (8) and passes through a second position (P2).

A microfluidic device system includes a channel having an entrance and an exit, a height at the entrance being greater than a height at the exit. The height of the channel may decrease continuously from the height at the entrance to the height at the exit. Cells or particles or beads traveling through the channel become trapped based on their size and/or deformability. A visual sensor captures images of the trapped cells or particles or beads, and image software analyzes the captured images to provide size and/or deformability and/or fluorescence information. A method of fabricating such a microfluidic device includes introducing a glass wafer to an etching solution at a specific rate such that a first end of the glass wafer is etched longer than other portions of the glass wafer.

The present disclosure provides, in one aspect, a target substance detection method in which a trapping substance that indirectly binds to both an immobilization side (support side) and a detection side (reporter side), which is a method for detecting a target substance in a sample. The method includes a reaction step of reacting the sample, a support including a first binding portion, a reporter substance including a second binding portion, a first trapping substance that includes a first binding partner portion capable of binding to the first binding portion and that can bind to the target substance, and a second trapping substance that includes a second binding partner portion capable of binding to the second binding portion and that can bind to the target substance; and a detection step of detecting a signal from the reporter substance.

Methods are provided for detecting the presence of monensin in animal feed using a competitive lateral flow assay. The methods allow for qualitative detection of monensin, as well as quantitative detection at a very high level of sensitivity, e.g., detection of 10 ppm or less in a feed sample. Competitive lateral flow assay strip devices are also provided for use in the methods.

An embodiment relates to a reagent buffer comprising a zwitterionic sulfonic solution, a non-ionic surfactant solution, and a buffer solution, wherein the reagent buffer has a pH range of about 7.0 to about 8.0 and a circular dichroism with an absorbance peak more than 0 at 190 nm and 225 nm. In an embodiment, the reagent buffer is configured to be a reagent buffer for a lateral flow immunoassay for detection of COVID-19.

Provided are a latex agglutination reagent containing polyoxazoline that is preferable for use in a latex agglutination reaction and has a high sensitization action of sensitivity while a non-specific reaction can be suppressed, and a method for measuring a target substance in a specimen by a latex agglutination method. A latex agglutination reagent used in a method for measuring a target substance in a specimen by a latex agglutination method using an insoluble carrier that carries an antibody or an antigen contains polyoxazoline having a repeating unit represented by the following General Formula

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(in Formula (1), R represents a hydrogen atom or a methyl group, an ethyl group, or a propyl group, and n is an integer of 100 or greater).

The present invention is directed to a pre-coated substrate, such as a slide, that is useful for immobilizing a sample. The invention is further provides methods of preparing such pre-coated substrates and methods of analyzing biological samples immobilized on such pre-coated substrate. The substrate is coated with a polycationic polymeric coating material specifically selected such that that coated substrate exhibits increased stability and prolonged shelf-life. Preferred polymeric coating materials include allylic or vinylic polymers having cationic groups thereon and having no more than a small percentage of peptidic monomeric linkages, particularly polydiallyldimethylammonium (PDDA).