A substrate comprising a microporous microstructure, an interlayer over at least a portion of the microstructure and a functional layer attached to the interlayer, the functional layer having functional sites with a density of at least 50 nanomoles/cm.sup.2.

A substrate comprising a microporous microstructure, an interlayer over at least a portion of the microstructure and a functional layer attached to the interlayer, the functional layer having functional sites with a density of at least 50 nanomoles/cm.sup.2.

The present invention relates to a novel polypeptide that binds selectively to mouse or rabbit immunoglobulin G. More specifically, the present invention relates to a polypeptide that binds to mouse or rabbit immunoglobulin G, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, a transformant introduced with the expression vector, a method of producing the polypeptide using the transformant, and a composition for immunoassay comprising the polypeptide. The novel peptide according to the present invention binds specifically to mouse or rabbit immunoglobulin G, can replace conventional expensive secondary immunoglobulin G, and can be used in various biological immunoassays. In addition, a conjugate of the polypeptide of the present invention and immunoglobulin G is useful for fabrication of various immunosensors/immunochips and for drug screening.

The present invention relates to a novel polypeptide that binds selectively to mouse or rabbit immunoglobulin G. More specifically, the present invention relates to a polypeptide that binds to mouse or rabbit immunoglobulin G, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, a transformant introduced with the expression vector, a method of producing the polypeptide using the transformant, and a composition for immunoassay comprising the polypeptide. The novel peptide according to the present invention binds specifically to mouse or rabbit immunoglobulin G, can replace conventional expensive secondary immunoglobulin G, and can be used in various biological immunoassays. In addition, a conjugate of the polypeptide of the present invention and immunoglobulin G is useful for fabrication of various immunosensors/immunochips and for drug screening.

The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R.sub.1-L.sub.1-N(R.sub.3)-L.sub.2-R, immobilized on a support, wherein R.sub.1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L.sub.1 is either a methylene group or a covalent bond; R.sub.2 is a five-or six-membered, substituted or non-substituted ring structure; L.sub.2 is either a methylene group or a covalent bond; R.sub.3 is a methyl group; and wherein if R.sub.1 is a hydroxyethyl group and L.sub.1 is a covalent bond, R.sub.2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R.sub.1-L.sub.1-N(R.sub.3)-L.sub.2-R, immobilized on a support, wherein R.sub.1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L.sub.1 is either a methylene group or a covalent bond; R.sub.2 is a five-or six-membered, substituted or non-substituted ring structure; L.sub.2 is either a methylene group or a covalent bond; R.sub.3 is a methyl group; and wherein if R.sub.1 is a hydroxyethyl group and L.sub.1 is a covalent bond, R.sub.2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.

Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.

Methods are provided for detecting antigen binding agents in samples. Aspects of the methods include detection of the aggregation of antigen binding agents with polynucleotide-bound antigens by sensitive proximity-based association of the antigen-bound polynucleotides. Aspects of the methods also include methods for the detection of such proximity-based association through nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.

The disclosure provides compositions comprising at least one assembly comprising a peptide and a major histocompatibility complex (MHC), wherein the peptide is an integral component of the MHC, wherein the peptide is attached to a surface at its C-terminus through a linker and wherein the peptide is synthesized on the surface. In certain embodiments, the compositions comprise a plurality of assemblies in a spatially-ordered array. The disclosure provides methods for making and using these compositions.