Provided are methods and compositions for the diagnosis and treatment of COVID-19, a disease caused by SARS-CoV-2 infection. More specifically, peptides that bind to SARS-CoV-2 are provided for use as diagnostic and therapeutic compositions in diagnosis, treatment and prevention of individuals contracting, or in danger of contracting COVID-19.

Provided are methods and compositions for the diagnosis and treatment of COVID-19, a disease caused by SARS-CoV-2 infection. More specifically, peptides that bind to SARS-CoV-2 are provided for use as diagnostic and therapeutic compositions in diagnosis, treatment and prevention of individuals contracting, or in danger of contracting COVID-19.

A complex having the structure of formula (I) ##STR00001##
is disclosed. L.sub.1, L.sub.2, L.sub.3, L.sub.4, and L.sub.5 may be or include independently metal-coordinating ligands selected from the group consisting of neutral ligands, anionic ligands, and mixed ligands, and combinations thereof. In a non-limiting embodiment, the complex is an N-heterocyclic carbene (NHC) Re(I) complex having an unsubstituted or substituted benzimidazol-2-ylidene ligand. The complex may be included in a pharmaceutical composition for treating gram (+) bacterial infections.

A complex having the structure of formula (I) ##STR00001##
is disclosed. L.sub.1, L.sub.2, L.sub.3, L.sub.4, and L.sub.5 may be or include independently metal-coordinating ligands selected from the group consisting of neutral ligands, anionic ligands, and mixed ligands, and combinations thereof. In a non-limiting embodiment, the complex is an N-heterocyclic carbene (NHC) Re(I) complex having an unsubstituted or substituted benzimidazol-2-ylidene ligand. The complex may be included in a pharmaceutical composition for treating gram (+) bacterial infections.

Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.

Provided is an immunochromatographic method that amplifies surface plasmon resonance without requiring a complicated preparation process or inspection procedure.

The immunochromatographic method detects an analyte by forming a complex wherein a resonant particle that causes surface plasmon resonance is accumulated on a separate carrier particle via the analyte, and capturing and detecting this complex on a test piece for an immunochromatographic method, In this method, the sensitivity is amplified as compared with when only a particle that causes surface plasmon resonance are used.

Provided is an immunochromatographic method that amplifies surface plasmon resonance without requiring a complicated preparation process or inspection procedure.

The immunochromatographic method detects an analyte by forming a complex wherein a resonant particle that causes surface plasmon resonance is accumulated on a separate carrier particle via the analyte, and capturing and detecting this complex on a test piece for an immunochromatographic method, In this method, the sensitivity is amplified as compared with when only a particle that causes surface plasmon resonance are used.

Metal-organic frameworks (MOFs) comprising amines on the organic linker can be used for cell targeting. In particular, primary amine groups represent one of the most versatile chemical moieties for conjugation to biologically relevant molecules, including antibodies and enzymes. Different chemical conjugation schemes can be used to conjugate biological molecules to the amino functionality on the organic linker. For example, carbodiimide chemistry can be used to link a primary amine to available carboxyl groups on the protein. For example, sulfhydryl crosslinking chemistry can be used via Traut's reagent scheme. As a demonstration of the invention, the ability of EpCAM antibody-targeted MOFs to bind to a human epithelial cell line (A549), a common target for imaging studies, was confirmed with confocal microscopy.

Metal-organic frameworks (MOFs) comprising amines on the organic linker can be used for cell targeting. In particular, primary amine groups represent one of the most versatile chemical moieties for conjugation to biologically relevant molecules, including antibodies and enzymes. Different chemical conjugation schemes can be used to conjugate biological molecules to the amino functionality on the organic linker. For example, carbodiimide chemistry can be used to link a primary amine to available carboxyl groups on the protein. For example, sulfhydryl crosslinking chemistry can be used via Traut's reagent scheme. As a demonstration of the invention, the ability of EpCAM antibody-targeted MOFs to bind to a human epithelial cell line (A549), a common target for imaging studies, was confirmed with confocal microscopy.

Embodiments of the disclosure concern methods of determining the effectiveness of immune cells, such as T cells, with particular chimeric antigen receptors. In specific embodiments, a synapse between the CAR and the tumor antigen is measured for structure, signaling, and functionality by imaging. As such, the quality of the synapse is determined and positively correlates with effectiveness of the particular CAR immune cells.