Disclosed herein is a system and method for transistor pathogen virus detector in which one embodiment may include a substrate layer, a silicon dioxide layer on the substrate layer, a nanocrystalline diamond layer on the silicon dioxide layer, a graphene oxide layer on the nanocrystalline diamond layer, fluorinated graphene oxide portions; and a linker layer, the linker layer including a plurality of pathogen receptors.

An in vitro experimental standard comprising sections cut from a homogenous cell block for use as a positive control for biomarkers in immunohistochemistry experiments. The homogenous cell block is produced using a three layered vertical apparatus to create an evenly distributed suspension of FFPE cells, wherein the cells are mixed with 3% agarose while still rotating within the apparatus's middle layer. The injection of the cell mixture into a mold creates a homogeneous cell block where each cell, or ratio of different types of cells, is evenly distributed. The cell mixture within the cell block may further comprise: a mixture of the same type of cell with different genetic modifications; a mixture of the same type of cell with different protein or nucleic acids expression; and a mixture of different types of cells with different genetic backgrounds, and/or different expression level of genes and/or proteins.

An in vitro experimental standard comprising sections cut from a homogenous cell block for use as a positive control for biomarkers in immunohistochemistry experiments. The homogenous cell block is produced using a three layered vertical apparatus to create an evenly distributed suspension of FFPE cells, wherein the cells are mixed with 3% agarose while still rotating within the apparatus's middle layer. The injection of the cell mixture into a mold creates a homogeneous cell block where each cell, or ratio of different types of cells, is evenly distributed. The cell mixture within the cell block may further comprise: a mixture of the same type of cell with different genetic modifications; a mixture of the same type of cell with different protein or nucleic acids expression; and a mixture of different types of cells with different genetic backgrounds, and/or different expression level of genes and/or proteins.

A preparation method and use of a BiOX/N-doped biochar nanocomposite, where X is I or Br is provided. The preparation method includes the following steps: step 1: preparation of an N-doped biochar; step 2: preparation of an acidified N-doped biochar; and step 3: preparation of the BiOX/N-doped biochar nanocomposite. In the present disclosure, a discarded crayfish shell, crab shell, or tofu residue is used as a raw material to prepare the BiOX/N-doped biochar nanocomposite, to realize the transformation of a renewable biological resource from waste into treasure. A photoelectric sensor is constructed based on the BiOX/N-doped biochar nanocomposite that can realize the detection of adenosine triphosphate (ATP) or Escherichia coli (E. coli).

A solid composition comprising a homogenous cell block able to be used as a positive control for biomarkers in immunohistochemistry experiments, such as slide scanning and image analysis. The homogenous cell block is produced using a three layered vertical apparatus to create an evenly distributed suspension of FFPE cells, wherein the cells are mixed with 3% agarose while still rotating within the apparatus's middle layer. The injection of the cell mixture into a mold creates a homogeneous cell block where each cell, or ratio of different types of cells, is evenly distributed. The cell mixture within the cell block may further comprise: a mixture of the same type of cell with different genetic modifications; a mixture of the same type of cell with different protein or nucleic acids expression; and a mixture of different types of cells with different genetic backgrounds, and/or different expression level of genes and/or proteins.

The present invention discloses a methionine adenosyltransferase (MAT) activity assay method and a kit for measuring MAT activity. A sample and relevant reagents are mixed in certain way that MAT-catalyzed reaction occurs efficiently. The reaction and the competitive ELISA that quantifies the product S-adenosylmethionine (SAM) are carried out simultaneously. The MAT activity is calculated as the amount of SAM produced per unit time. SAM is calculated through spectral absorbance of the SAM produced and comparing it to that of the standard. The method of SAM quantification is via tracer-labelled anti-SAM antibody or SAM (or SAM analog) antigen through competitive ELISA, so that the produced SAM competes with the SAM antigen for binding anti-SAM antibody. The method and the kit described in the present invention are more sensitive, accurate, reliable, straightforward, easier and faster. The method was used to measure the MAT activities of normal and cancerous liver cells.

The present invention discloses a methionine adenosyltransferase (MAT) activity assay method and a kit for measuring MAT activity. A sample and relevant reagents are mixed in certain way that MAT-catalyzed reaction occurs efficiently. The reaction and the competitive ELISA that quantifies the product S-adenosylmethionine (SAM) are carried out simultaneously. The MAT activity is calculated as the amount of SAM produced per unit time. SAM is calculated through spectral absorbance of the SAM produced and comparing it to that of the standard. The method of SAM quantification is via tracer-labelled anti-SAM antibody or SAM (or SAM analog) antigen through competitive ELISA, so that the produced SAM competes with the SAM antigen for binding anti-SAM antibody. The method and the kit described in the present invention are more sensitive, accurate, reliable, straightforward, easier and faster. The method was used to measure the MAT activities of normal and cancerous liver cells.

The present invention provides a method and a system based on a multi-gate field effect transistor for sensing molecules in a gas or liquid sample. The said FET transistor comprises dual gate lateral electrodes (and optionally a back gate electrode) located on the two sides of an active region, and a sensing surface on top of the said active region. Appling voltages to the lateral gate electrodes, creates a conductive channel in the active region, wherein the width and the lateral position of the said channel can be controlled. Enhanced sensing sensitivity is achieved by measuring the channels conductivity at a plurality of positions in the lateral direction. The use of an array of the said FTE for electronic nose is also disclosed.

Provided are site specific chemically modified nanopore devices and methods for manufacturing and using them. Site specific chemically modified nanopore devices can be used for analyte sensing and analysis, for example.

Techniques for enhanced fluorescence include a functionalized substrate for a target optical frequency comprising a one dimensional photonic crystal that is functionalized with a bioactive target molecule that has an affinity for a particular analytic. The one dimensional photonic crystal includes a plurality of dielectric layers including a plurality of high index of refraction layers alternating with a plurality of low index of refraction layers. The thickness of each layer is within a factor of four of a wavelength of the optical frequency in the layer. For emissions from a fluorophore bound to the target molecule and excited by incident light, there is an emission intensity maximum centered at an angle independent of the direction of the incident light.