Methods and devices are provided for the detection and characterization of circulating cells in a blood sample. Such method can include depositing a sample of a bodily fluid on a device comprising carbon nanotubes, wherein the surfaces of the carbon nanotubes are not functionalized; and detecting target cells adhered to the carbon nanotubes.

The invention includes a bioelectronic interface comprising a self-assembling unit, wherein the self-assembling unit comprises a variant GPCR fusion protein bound to an S-layer fusion protein. The invention also encompasses a biosensor or device comprising the bioelectronic interface and methods of screening for a ligand of a GPCR.

The invention includes a bioelectronic interface comprising a self-assembling unit, wherein the self-assembling unit comprises a variant GPCR fusion protein bound to an S-layer fusion protein. The invention also encompasses a biosensor or device comprising the bioelectronic interface and methods of screening for a ligand of a GPCR.

Porous zirconia particles exhibit high specificity to a protein to be immobilized thereto and are used in immobilization of the protein. The porous zirconia particles have a pore diameter D50, at which a ratio of a cumulative pore volume to a total pore volume is 50%, the pore diameter D50 being in a range of 3.20 nm or more and 6.50 nm or less; and a pore diameter D90, at which a ratio of a cumulative pore volume to a total pore volume is 90%, the pore diameter D90 being in a range of 10.50 nm or more and 100.00 nm or less. The total pore volume of the particles is greater than 0.10 cm.sup.3/g. D50, D90, and the total pore volume are determined based on a pore diameter distribution measured through a BET method.

An example binding assay includes a plurality of sub-regions, a plurality of synthetic compounds on beads, wherein each of the plurality of sub-regions includes one of the plurality of synthetic compounds, a biological target labeled with a first detectable label in each of the plurality sub-regions, and a biological counter target labeled with a second detectable label in each of the plurality of sub-regions. The biological counter target is configured to bind to the biological target when the biological target is in a first orientation. And, wherein a first subset of the plurality of synthetic compounds bind to the biological target and effect interactions between the biological target and the biological counter target.

An example binding assay includes a plurality of sub-regions, a plurality of synthetic compounds on beads, wherein each of the plurality of sub-regions includes one of the plurality of synthetic compounds, a biological target labeled with a first detectable label in each of the plurality sub-regions, and a biological counter target labeled with a second detectable label in each of the plurality of sub-regions. The biological counter target is configured to bind to the biological target when the biological target is in a first orientation. And, wherein a first subset of the plurality of synthetic compounds bind to the biological target and effect interactions between the biological target and the biological counter target.

The present invention relates to a nanoparticle complex for isolation of a biological target and a preparation method therefor and, more specifically, to a nanoparticle complex in which a receptor is conjugated to a polymer-coated nanoparticle, a preparation method therefor, and a use thereof.

The present invention relates to a nanoparticle complex for isolation of a biological target and a preparation method therefor and, more specifically, to a nanoparticle complex in which a receptor is conjugated to a polymer-coated nanoparticle, a preparation method therefor, and a use thereof.

In one aspect, a method of detecting a pathogen, e.g., listeria bacterium, chlamydia bacteria, gonorrhea bacteria and/or HPV, in a sample is disclosed, which comprises bringing a sample into contact with a graphene layer functionalized with an antibody exhibiting specific binding to the pathogen, monitoring electrical resistance of said antibody-functionalized graphene layer in response to interaction with said sample, and detecting presence of the pathogen in said sample by detecting a change in said electrical resistance indicative of interaction of the pathogen with said antibody-functionalized graphene layer. For example, a decrease of the electrical resistance of the graphene layer can indicate the presence of the pathogen in the sample under study. In some embodiments, a method according to the present teachings is capable of detecting pathogens, such as listeria bacteria, chlamydia bacteria, gonorrhea bacteria and HPV in a sample at a concentration as low as 4 cfu per 100 grams of a sample.

The present invention provides a method and a system based on a multi-gate field effect transistor for sensing molecules in a gas or liquid sample. The said FET transistor comprises dual gate lateral electrodes (and optionally a back gate electrode) located on the two sides of an active region, and a sensing surface on top of the said active region. Appling voltages to the lateral gate electrodes, creates a conductive channel in the active region, wherein the width and the lateral position of the said channel can be controlled. Enhanced sensing sensitivity is achieved by measuring the channels conductivity at a plurality of positions in the lateral direction. The use of an array of the said FTE for electronic nose is also disclosed.