This disclosure provides a system for detecting rare cells. The system includes a substrate, an extension coupled to the substrate and extending outwardly from the substrate, and a functionalized graphene oxide disposed on the extension. This disclosure also provides a method for detecting rare cells using the system of this disclosure. The method includes the steps of providing the system and introducing a sample of bodily fluid to the system such that the sample interacts with the functionalized graphene oxide.

The invention is related to the field of biotechnology, specifically to the investigation of biomolecular interactions and sensing of biomolecules using a surface plasmon resonance. The biological sensor and a method of its production based on the thin films of graphene, graphene oxide, or single-walled or multi-walled carbon nanotubes are described. The technical results of the invention are a high sensitivity of the biosensor in combination with a high biospecificity; an expansion of the range of device applications; the protection of the metal film from an environmental exposure; the possibility to detect large biological objects. The proposed device and method of its production can be used for monitoring and recording of the concentration of chemical and biochemical substances and for the definition of parameters of biomolecular reactions in different industrial processes using biological materials, the invention can be also used in the pharmaceutical industry for the investigation of pharmacological properties and for the determination of a chemical composition of developing drugs, and also it can be used in processes of quality control of food products.

The invention is related to the field of biotechnology, specifically to the investigation of biomolecular interactions and sensing of biomolecules using a surface plasmon resonance. The biological sensor and a method of its production based on the thin films of graphene, graphene oxide, or single-walled or multi-walled carbon nanotubes are described. The technical results of the invention are a high sensitivity of the biosensor in combination with a high biospecificity; an expansion of the range of device applications; the protection of the metal film from an environmental exposure; the possibility to detect large biological objects. The proposed device and method of its production can be used for monitoring and recording of the concentration of chemical and biochemical substances and for the definition of parameters of biomolecular reactions in different industrial processes using biological materials, the invention can be also used in the pharmaceutical industry for the investigation of pharmacological properties and for the determination of a chemical composition of developing drugs, and also it can be used in processes of quality control of food products.

A system and method for sample droplet processing, the system including a substrate, an electrode array network coupled to the substrate and configured to provide a pattern of controlled electric fields for manipulation of the set of sample droplets; a first layer in communication with the electrode array network, the first layer separating the electrode array network from fluid of the set of sample droplets; and a second layer opposing the first layer and displaced from the first layer to define a region wherein droplets of the set of sample droplets can reside. In some variations, the system can additionally include an electronics subsystem coupled to at least one of the substrate and the electrode array network, and a control module in communication with the electronics subsystem, wherein the control module generates and manipulates the pattern of controlled electric fields.

An ultra-high sensitivity dual-gated biosensor based on an MOS transistor, which is applicable to detection of a series of early tumors. The sensor is prepared and processed by using SOI wafers, and a unique dual-gated structure is realized by ion implantation technique. The sensor is prepared by an ultraviolet lithography combined with an NLD etching method, realizing trace, instant and marker-free detection of tumor markers. The method detects a change in capacitance in the channel during binding of antigen antibodies. The detection method involved in the invention is more stable and strong in anti-interference, can meet the demands in the aspect of detection range and sensitivity, and especially has extremely outstanding detection sensitivity, and can detect a sample with a lowest concentration in the range of 1 fg/ml1 ng/ml.

One aspect of the present disclosure relates to an immunochromatographic detection method for a target antigen in a biological sample, comprising the step of joining a linker having a first antibody and a quantum dot bead having a second antibody, with respect to the target antigen. By using the quantum dot beads and the linker, the method can successfully amplify detection strength and significantly increase detection sensitivity through a simple process without causing the loss of antigens participating in the detection when using only quantum dot beads. Furthermore, the present disclosure can significantly amplify detection strength without an additional washing step, thus enabling excellent detection and identification of a physiological substance in a biological sample, even in an actual product, and can be used to provide a product with a competitive price.

A problem to be solved by the present invention is to provide a method of assisting diagnosis of the health condition of a subject by correcting variation in an amount of a collected disease marker, which is caused by variation in the skin barrier function of the subject, and acquiring information reflecting the health condition of the subject accurately, and to solve the problem, the present invention provides a method of assisting diagnosis of a disease in a subject using a disease marker and a reference marker, the method including: applying, to a skin surface of the subject, a sheet capable of generating an attractive force due to electrostatic interaction between the sheet and each of the disease and reference markers; detaching the sheet from the skin surface; measuring the amount of each of the disease and reference markers that are attached to the sheet; and acquiring information on the disease in the subject based on a value obtained by correcting the measured amount of the disease marker with the measured amount of the reference marker, wherein the reference marker is annexin A2.

Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.

Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.

Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.