Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed.

The present invention relates to a peptide for detecting HE4 and use thereof and, more specifically, to a peptide for detecting HE4 that specifically binds to HE4 and use thereof for diagnosing ovarian cancer. The peptide that specifically binds to HE4, according to the present invention, can detect HE4 with high sensitivity and accuracy. In addition, when the peptide is expressed in the outer coat of a bacteriophage and constructed into a bacteriophage nano self-assembly, the peptide can conveniently detect HE4 from a sample through observation of a color change with a simple imaging device such as a smartphone, and in particular, the peptide can detect HE4 included in a small amount in saliva, and thus has the advantage of being very effectively used in early diagnosis of ovarian cancer.

Provided herein are biochip, system and method for detection of a marker molecule in a sample, the biochip including one or more chambers, each chamber containing a synthetic biosensor module and a detection module, wherein the synthetic biosensor module comprises a genetically modified bacteria expressing a receptor capable of binding the marker molecule, and a reporter gene, wherein expression of the reporter gene is induced by the binding of the marker molecule to the receptor; and wherein the detection module produces an output signal indicative of presence of the marker molecule in the sample.

Provided herein are biochip, system and method for detection of a marker molecule in a sample, the biochip including one or more chambers, each chamber containing a synthetic biosensor module and a detection module, wherein the synthetic biosensor module comprises a genetically modified bacteria expressing a receptor capable of binding the marker molecule, and a reporter gene, wherein expression of the reporter gene is induced by the binding of the marker molecule to the receptor; and wherein the detection module produces an output signal indicative of presence of the marker molecule in the sample.

A biosensor system for the detection of target analytes that includes a living biological cell of a predetermined type; a signal-generating reporter associated with the living biological cell; a signal transduction pathway or other activator mechanism or means associated with the signal-generating reporter; a universal detector element associated with the activator mechanism; and an analyte binding element associated with the universal detector element, wherein the analyte binding element is specific to both the universal detector element and a target analyte.

A biosensor system for the detection of target analytes that includes a living biological cell of a predetermined type; a signal-generating reporter associated with the living biological cell; a signal transduction pathway or other activator mechanism or means associated with the signal-generating reporter; a universal detector element associated with the activator mechanism; and an analyte binding element associated with the universal detector element, wherein the analyte binding element is specific to both the universal detector element and a target analyte.

The present invention relates to a derivative bacterial strain of an avirulent, non-pathogenic Clostridium ghonii that is capable of arresting the growth of, regressing or destroying one or more solid tumors. The present invention further relates to a composition comprising the derivative bacterial strain for targeted lysis of solid tumors. The present invention further relates to methods of producing derivative strains of C. ghonii by serial culturing in tumor bearing animals.

The present invention relates to a peptide for detecting CA125 and use thereof and, more specifically, to a peptide for detecting CA125 that specifically binds to CA125 and use thereof for diagnosing ovarian cancer. The peptide that specifically binds to CA125, according to the present invention, can detect CA125 with high sensitivity and accuracy. In addition, when the peptide is expressed in the outer coat of a bacteriophage and constructed into a bacteriophage nano self-assembly, the peptide can conveniently detect CA125 from a sample through observation of a color change with a simple imaging device such as a smartphone, and in particular, the peptide can detect CA125 included in a small amount in saliva, and thus has the advantage of being very effectively used in early diagnosis of ovarian cancer.

The present invention relates to a peptide for detecting CA125 and use thereof and, more specifically, to a peptide for detecting CA125 that specifically binds to CA125 and use thereof for diagnosing ovarian cancer. The peptide that specifically binds to CA125, according to the present invention, can detect CA125 with high sensitivity and accuracy. In addition, when the peptide is expressed in the outer coat of a bacteriophage and constructed into a bacteriophage nano self-assembly, the peptide can conveniently detect CA125 from a sample through observation of a color change with a simple imaging device such as a smartphone, and in particular, the peptide can detect CA125 included in a small amount in saliva, and thus has the advantage of being very effectively used in early diagnosis of ovarian cancer.

Provided herein are genetically modified microbes. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide comprising a pheromone-responsive region. In one embodiment, the pheromone-responsive region is derived from a conjugative plasmid from a member of the genus Enterococcus spp. The pheromone-responsive region includes a pheromone-responsive promoter and an operably linked coding region encoding an antimicrobial peptide. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide comprising a promoter and an operably linked coding sequence encoding an antimicrobial peptide, where expression of the coding region is controlled by a modulator polypeptide and is altered by a modulating agent, and where the coding region encodes an antimicrobial peptide. Also provided herein are methods of using the genetically modified microbes, including methods for inhibiting growth of an Enterococcus spp., a pathogenic E. coli, or a pathogenic Salmonella spp., for treating a subject, and for modifying a subject's gastrointestinal microflora.