The present disclosure provides an optical readout imaging system may include first electrode, a thin film disposed on the first electrode, a biomolecule transfer layer disposed on the thin film, and a second electrode disposed on the biomolecule transfer layer. The present disclosure also provides a biochemical detection method using the optical readout imaging system.

The present invention includes methods for detecting benign to malignant transformation of a cancer in a subject, comprising the steps of: collecting a sample from the subject prior to electrophoretic protein separation; activating electrophoretically separated ENOX2 transcript variants with an ENOX2 electron donor; and detecting the presence of the one or more activated ENOX2 transcript variants using a pan-ENOX2 detectable binding reagent, wherein the presence of one or more activated ENOX2 transcript variants in the sample is indicative of the malignant transformation of the cancer, whereby a 10 to 100 fold increase in detection sensitivity is obtained for the one or more activated ENOX2 transcript variants when compared to an equivalent non-activated ENOX2 transcript variant.

The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.

The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.

An ITP-based system and a method are provided. ITP is used to focus a sample of interest and deliver a high concentration target to a pre-functionalized surface comprising immobilized probes, thus enabling rapid reaction at the sensor site.

The invention regards the use of triiodothyronine sulfate, commonly named T.sub.3S, as a medicament having thyromimetic activity for the treatment of pathologies due to organic deficiency of triiodothyronine (T.sub.3), as such or in association with thyroxine (T.sub.4), and pharmaceutical formulations for oral administration thereof.

Disclosed is a method for analyzing biological samples by immunofixation electrophoresis which involves immunodisplacement of target component(s) that may be present in the assayed biological sample, the target component(s) amounting to interfering component(s) when interpretation of the immunofixation results is considered. The immunodisplacement is carried out on an electrophoretic support that is preferably a gel, as defined herein. Accordingly, the invention provides IFE using an antibody or antibodies which is(are) modified (modified antibody) to bear additional negative electric charges, the modified antibody(ies) having antigenic specificity for a predetermined target immunoglobulin.

Disclosed is a method for analyzing biological samples by immunofixation electrophoresis which involves immunodisplacement of target component(s) that may be present in the assayed biological sample, the target component(s) amounting to interfering component(s) when interpretation of the immunofixation results is considered. The immunodisplacement is carried out on an electrophoretic support that is preferably a gel, as defined herein. Accordingly, the invention provides IFE using an antibody or antibodies which is(are) modified (modified antibody) to bear additional negative electric charges, the modified antibody(ies) having antigenic specificity for a predetermined target immunoglobulin.

An electrophoretic spectroscopic imaging device for real-time spatially-resolved spectroscopic imaging of reagents and reaction-products resulting from electrophoretic collisions of reagents includes an electrophoresis component; a pair of electrodes; an illumination source; a spectroscopic-imaging device; and a computing device. An electrophoretic gel includes a matrix of porous solid material; and an electrolyte solution disposed within pores of the matrix. A method of electrophoretically colliding reagents includes providing a matrix that is a porous solid material having continuously interconnected pore regions that are filled with an electrolyte solution; loading a first reagent; loading a second reagent; and applying an electric field to the matrix loaded with the first reagent and the second reagent.

An electrophoretic spectroscopic imaging device for real-time spatially-resolved spectroscopic imaging of reagents and reaction-products resulting from electrophoretic collisions of reagents includes an electrophoresis component; a pair of electrodes; an illumination source; a spectroscopic-imaging device; and a computing device. An electrophoretic gel includes a matrix of porous solid material; and an electrolyte solution disposed within pores of the matrix. A method of electrophoretically colliding reagents includes providing a matrix that is a porous solid material having continuously interconnected pore regions that are filled with an electrolyte solution; loading a first reagent; loading a second reagent; and applying an electric field to the matrix loaded with the first reagent and the second reagent.