A method of detecting an endotoxin of detecting an endotoxin from a test subject sample using a fluorescent substance having a structure in which a fluorescent site and a recognition site are connected by a spacer, wherein the recognition site recognizes a specific site of a molecular structure of an endotoxin.

A method to predict a propensity of an individual to develop atopic dermatitis is disclosed. The method, which involves use of biological markers, can also be used to evaluate the efficacy of a composition to treat atopic dermatitis.

A method of determining susceptibility to COVID-19 infection comprising the steps of providing a stool sample, and determining an amount of Bifidobacterium, Clostridium, Faecalibacterium, Faecalibacterium prausnitzii, Ruminococcus, and Subdoligranulum in the stool sample. A method of determining susceptibility to COVID-19 infection comprising the steps of providing a stool sample, and determining an amount of Bifidobacterium and Faecalibacterium, in the stool sample. A method of reducing the severity of COVID-19 infection comprising the steps of providing the individual, and administering at least one of the following: Bifidobacterium and or Faecalibacterium. A method of reducing the risk of infection of COVID-19 comprising the steps of providing the individual, and administering Bifidobacterium and or Faecalibacterium. A method of increasing an amount of Bifidobacterium and Faecalibacterium in an individual, the method comprising the steps of providing the individual; and administering one or more of the following: Vitamin C, doxycycline, Zinc, and Ivermectin.

A magnetic separation device has a membrane having a plurality of pores, a magnetically soft material layer disposed on the membrane, and a passivation layer disposed on the magnetically soft material layer. The magnetic separation device may be part of a microfluidic device having a lateral flow channel and a vertical flow magnetic separation filter. The magnetic separation device may be used to separate magnetically tagged particles, such as cells.

Presented herein, in certain embodiments, are compositions comprising antibody binding agents that specifically bind to A. baumannii and inhibit and/or block A. baumannii infection, and uses thereof.

Sensors arrays that include indicators arranged in a pattern on a substrate. The indicators include nucleophilic indicators. In some examples, the sensor arrays with multiple nucleophilic indicators provide superior detection and identification of microorganisms, cancer biomarkers, formaldehyde, organophosphates, and aldehydes.

The present disclosure relates to proteins comprising a target-binding domain for detection of a target of interest, methods, compositions and kits thereof.

There is provided compositions for the detection of target cellular material, the composition comprising: a chemically modified substrate; at least one binding moiety, wherein the binding moiety comprises a glycan; and at least one linker covalently linked to the substrate and the binding moiety by a first and second bond. There is also provided methods of preparation of such compositions and methods for detection of target cellular material, such as for detection of pathogenic microorganisms.

Devices, systems, and methods are provided for magnetic focus enhanced lateral flow assays. The devices and systems are ultrasensitive and provide for the visual detection of the presence or absence of one or more target analytes—which may include pathogens, proteins, or even molecules smaller than the foregoing—even when such analytes are only present in very limited amounts. The devices and systems include an immunostrip with a magnet positioned adjacent thereto, and magnetic probes specific to a target analyte that bind to the target analyte with specificity if present within a fluid sample to be tested. Methods are also provided for detecting one or more target analytes using magnetic focus, such methods including a step of controlling movement of a target analyte complex on an immunostrip incorporating a magnetic field, where such control slows a flow of the target analyte complex through a capture area on the immunostrip.

Described are assays, associated assay kits, and associated methods for diagnosing an infection in a subject. The assay includes a first binding molecule that specifically binds an inflammatory marker (i.e., neutrophil gelatinase-associated lipocalin (NGAL) homodimer) in a sample taken from the subject, and a second binding molecule that binds a marker indicative of the presence of a pathogen in the sample. The assay and methods are for the diagnosis of peritonitis in peritoneal dialysis patients.