Reagents and methods to use said reagents for detection of markers of various aspects of Lyme disease have been identified in biological fluids thus permitting diagnosis or classification of Lyme disease subjects as well as indicating suitable methods of treatment.

A method of monitoring acute hepatopancreatic necrosis disease in shrimp is disclosed. More specifically, detection of the presence of alkaline phosphatase Phox enzyme, or, of the PiRA.sup.VP and/or the PirB.sup.VP toxins of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus correlates with non-virulence or virulence, respectively, of the bacterium is disclosed. Hence, an assay capable of detecting the Phox enzyme and/or the toxins could be very useful to monitor the disease in shrimp.

The invention relates to the field of biomedicine, and an anti-pertussis toxin (PT) single domain antibody and derivative protein thereof are disclosed. Specifically, a pertussis toxin-binding protein and use thereof are disclosed.

This disclosure relates generally to detection of pathogens from a gaseous mixture associated with secretions. Conventional methods typically involve invasive or biohazardous techniques, the requirement of quantity limits utility of several natural secretions, there is a dependency on immunological reactions to develop in a subject being monitored resulting in long time taken for detecting pathogens, which increases risk to health and environment. There is also reduced specificity and sensitivity considering the dependency on signature identification or training of machine learning models. Again, prior art focusses on designing antibodies for a particular type of sensor which is challenging when dealing with natural immunoglobulin. The present disclosure addresses these challenges by enabling identification of a most viable sensor for the natural immunoglobin, the viability being based on mathematical representations of the relationship between a sensor and the immunoglobulin using an ontology of domain knowledge associated with pathogens, technology, processing and detection.

A method for determining the susceptibility of a bacteria to an antibiotic, comprising transferring one portion of a sample containing living bacterial cells into a bacterial growth medium to create a control sample; transferring another portion of the sample into a bacterial growth medium to which a predetermined amount of antibiotic or predetermined amount of a library of antibiotics has been added to create a test sample; adding an alkyne-modified non-canonical amino acid to both the control sample and test sample during bacterial growth, wherein the alkyne-modified non-canonical amino acid incorporates into surface proteins, internal proteins, or both; reacting the alkyne-containing proteins with an azide-modified detection molecule using click-chemistry to label the cells; detecting the labeled cells using a method that generates a detectable signal; and comparing the signal generated by the control sample to the signal generated by the test sample, wherein a decrease in detectable signal between the control sample and the test sample is indicative of susceptibility of the living bacteria to the predetermined antibiotic or predetermined library of antibiotics.

The disclosure relates to a biosensor on the bacterial cell surface for sensing and responding to extracellular molecules. Related methods of using the bacterial cells to inducibly express a protein of interest and/or a bioactive RNA molecule in a subject are disclosed. Related methods of using the bacterial cells to inducibly express a protein of interest or to detect a target of interest in a sample are also disclosed.

This disclosure provides isolated or recombinant polypeptides that are useful to vaccinate individuals suffering from chronic/recurrent biofilm disease or as a therapeutic for those with an existing infection. The individual's immune system will then naturally generate antibodies which prevent or clear these bacteria from the host by interfering with the construction and or maintenance of a functional protective biofilm. Alternatively, antibodies to the polypeptides can be administered to treat or prevent infection. Bacteria that cannot form functional biofilms are more readily cleared by the remainder of the host's immune system and/or traditional antibiotics.

The present invention provides a whole blood sample detection method, device and system using a fluidic diffraction chip, including: injecting a whole blood sample through a diffraction chip; rinsing the diffraction chip; emitting a laser light source through the diffraction chip, wherein the wavelength range of the laser light source is 400 nm to 700 nm, the laser power density range of the diffraction chip is 2 mW/cm.sup.2 to 2000 mW/cm.sup.2, and a laser diffraction signal is received on the opposite side of the laser transmitter. The attenuation of the laser diffraction signal calculates the number of a test target. The method, device and system of the present invention can detect the number and status of cells or bacteria without labeling of cells or bacteria.

A marker for determining a mental disease is provided. The marker can be used in an objective diagnosis of such a mental disease. The marker contains one or more enterobacteria of Bifidobacterium, Lactobacillus, Lactobacillus brevis, Lactobacillus reuteri subgroup, Lactobacillus sakei subgroup, Atopobium cluster, Bacteroides fragilis group, Enterococcus, Clostridium coccoides group, Clostridium leptum subgroup, Staphylococcus, Clostridium perfringens, and Enterobacteriaceae.

The disclosure provides for a polyclonal antibody that specifically detects Acinetobacter baumannii, a multi-drug resistant (MDR) bacterial pathogen, and uses thereof, including as diagnostic tests and for immunoassays to be used in therapeutic decision-making or research experiments.