The present invention relates to a method and system for screening and separating biological specimens. The screening and separating method and system provide reliable results based on information related to the types, shapes, and positions of labeled products provided by an agent for accurate screening. In addition, the information related to the types, shapes, and positions can be combined to determine objective indices for the state of a target specimen, enabling rapid selection of the target specimen. The method and system for screening and separating biological specimens according to the present invention use an agent optimized to accurately and effectively create information on the selection of a target specimen and process images based on information on images stored in a server, enabling sensitive and highly reproducible screening evaluation.

Embodiments of the present disclosure provide a target capturing apparatus and a manufacturing method thereof, and a target detecting method. The target capturing apparatus includes a cavity structure, the cavity structure includes: an inlet portion, an outlet portion and a capture region positioned between the inlet portion and the outlet portion, and the capture region includes a capture component, and a combination specifically combined with a to-be-captured target is included in the capture component so as to capture the target in a sample entering the cavity structure.

Described are various embodiments of methods, devices, systems and kits for magnetic levitation-based separation of mixtures or populations of particles that include various types of particles. Some embodiments of such methods, devices, systems and kits are useful for magnetic levitation-based separation of mixtures or populations of cells that include various cell types. Some other embodiments of the described methods, devices, systems and kits are useful for magnetic levitation-based separation of mixtures or population of cellular or mixtures or population of biological molecules.

Described are various embodiments of methods, devices, systems and kits for magnetic levitation-based separation of mixtures or populations of particles that include various types of particles. Some embodiments of such methods, devices, systems and kits are useful for magnetic levitation-based separation of mixtures or populations of cells that include various cell types. Some other embodiments of the described methods, devices, systems and kits are useful for magnetic levitation-based separation of mixtures or population of cellular or mixtures or population of biological molecules.

Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.

The present invention relates to the field of short bowel syndrome. More specifically, the present invention provides methods and compositions useful for assaying for intestinal adaptation. In a specific embodiment, a method for treating a patient having SBS who is undergoing parenteral nutrition comprises the steps of (a) measuring lipocalin-2 (LCN2) in a sample obtained from the patient; and (b) reducing or eliminating parenteral nutrition if the measured level of LCN2 is below a control level or treating the patient with IL-22, a LCN2 inhibitor and/or an AHR agonist if the measured level of LCN2 is above the control level.

A method for screening for target cells, a corresponding test kit and a use thereof, a method for screening cells, and a biological culture chip and a preparation method therefor and a use thereof. The method for screening target cells comprises: culturing said candidate single cells within a culture chamber provided with a signal screening layer, the signal screening layer comprising signal molecules for specific recognition of target molecules; on the basis of signals of the signal molecules, selecting target cells suitable for secreting the target molecules. The method for screening cells comprises: arranging candidate cells in a culture chamber to form target antibody-antigen-signal antibody complexes; on the basis of signals of signal molecules connected to the signal antibodies, determining whether the candidate cells are target cells. The biological culture chip comprises a matrix (100), and a biological culture space arranged on the surface of the matrix (100) and used for culturing biological cells.

The present invention provides anti-C1q antibodies and methods of using the same.

The present disclosure relates to a dissociation reagent for tumor tissues. The dissociation reagent does not contain collagenase or trypsin but further contains hyaluronidase or a mixture of hyaluronidase and DNase I. The present disclosure also relates to use of the dissociation reagent in dispersing tumor tissues and detecting expression level of molecular markers on cell surface by flow cytometry. The dissociation reagent of the present disclosure does not cause degradation of molecular markers on cell surface such as CD8, PD-1, Tim-3, Lag-3 and the like, thus does not affect downstream assays.

Provided are affinity-based methods of isolating biological entities via a surface antigen from a sample with non-chromatographic and chromatographic methods being provided. Also provided is a dextran polymer, kits for use in the method of isolating a biological entity and an apparatus for performing the methods.