Embodiments may include a virumeter for rapid detection of COVID-19 infection and assessment of immunity to the virus. For example, a device for detecting primary antibodies to a pathogen or the pathogen may comprise a cartridge to receive a test sample from the person, the cartridge comprising at least one chamber to receive the test sample, first apparatus to mix at least one first reagent reactive to presence of the primary antibodies to the pathogen or the pathogen, second apparatus to mix at least one second reagent including a fluorescent compound with the test sample reactive to presence of the at least one first reagent having reacted to presence of the primary antibodies to the pathogen or the pathogen, and circuitry to determine presence of primary antibodies to the pathogen or the pathogen by detecting reaction of the second reagent by determining fluorescence of the fluorescent compound.

The present disclosure an ELISA-based assay that uses a glycosylated polypeptide fragment derived from the SARS-CoV-2 spike protein (Covid-19) receptor binding domain (S1RBD) that has affinity for the extracellular domain of Angiotensin Converting Enzyme 2 (ACE2). The S1RBD polypeptide is generated by expression of an encoding nucleic acid by a human cell expression system resulting in glycosylation of the expressed spike receptor binding domain (S1RBD) protein at least at the N343 N-glycosylation site thereof, and which surprisingly and significantly increases the affinity of the S1RBD for ACE2, provides a significant increase in the sensitivity of the assay compared to other known assays.

This disclosure provides antibodies that are derived from CFR3022 and that can be administered to an individual that is infected with a virus. Antibodies herein can be capable of treating or curing the virus, and which may provide protection against the virus for up to several weeks. Antibodies herein can be used to diagnose a SARS CoV 2 infection.

Described is a method to detect an analyte in an analytical toilet that includes the steps of receiving excreta in a bowl, transporting a measured sample of the excreta through a passage and bringing the sample into contact with a sensor. The sensor comprises a FET configured to interact with an analyte in the excreta. When the sample is brought into contact with the sensor, the sensor indicates the presence of the analyte by a distinct electric signal.

Provided herein is a test system comprising severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor binding domain (RBD) antigen, SARS-CoV-2 S2 antigen, and binding moieties that specifically bind to human IgG, human IgA, and human IgM. Also provided are methods of detecting SARS-CoV-2 antibodies in a sample using the test system.

The present disclosure provides a test kit for simultaneously detecting a plurality of analytes, in which the test kit includes a lysis solution and a test strip. The lysis solution includes a salt, a surfactant, a stabilizer, and a buffer solution. The test strip includes a sample pad, a conjugation pad, a cellulose membrane, and a water-absorbing pad sequentially arranged on a support plate. The conjugation pad includes a conjugation pad solution and a plurality of antibody-conjugated microspheres, and the antibody-conjugated microspheres recognize plurality of analytes. The test kit of the present disclosure achieves an effect of simultaneously detecting more than or equal to four analytes even though the conjugation pad has a limited capacity of the antibody by preparing the lysis solution with appropriate ingredients and improving a formulation of the solution contained in the test strip.

Stealth adapted viruses differ from the conventional viruses from which they are derived in not evoking an inflammatory response. This can occur because of the deletion or mutation of the genes coding for the relatively few virus components, which are normally targeted by the cellular immune system. As part of the stealth adaptation process, exchanges can occur between some and possibly all of the sequences of the initiating virus and sequences of both cellular and bacteria origin. A description is provided on the culturing of stealth adapted viruses. A characteristic feature of cultured stealth adapted virus infected cells is the accumulation of intracellular materials, which will fluoresce under ultraviolet (UV) light in the presence of certain dyes including neutral red and acridine orange.

Embodiments of the present disclosure relate generally to compositions and methods for the treatment and/or prevention of pathogenic viral infections, e.g., coronavirus infections. In particular, the present disclosure provides human plasma compositions and immunoglobulin prepared therefrom containing select antibody titers specific for coronavirus (e.g., SARS CoV-2), methods of identifying human donors and donor samples for use in the compositions, methods of manufacturing the compositions, and methods of utilizing the compositions for prophylactic administration and/or therapeutic treatment (e.g., passive immunization or immune-prophylaxis).

Immunosensors according to present embodiments combine a sandwich bioassay with an electrochemical surface plasmon resonance device for electrochemical detection of analytes from a sample, whereby a coated substrate for receiving an electroactive probe may be located in a flow cell, and the coated substrate comprises a first layer which is a silver (Ag) layer and a second layer which is a gold (Au) layer arranged so that the gold layer isolates the silver layer from an operating environment.

Provided herein are methods and systems for low-cost, low-equipment detection of pathogens in biological sample. In particular, provided herein is a low-cost method for detecting norovirus that provides reliable, visible test with femtomolar, attomolar, and zeptomolar detection limits and that uses materials suitable for deployment of the methods in the field.