The invention relates to multispecific antibodies, and antigen binding fragments thereof, that specifically bind to distinct Zika virus epitopes and potently neutralize infection of ZIKV. The invention also relates to nucleic acids that encode such antibodies and antibody fragments. In addition, the invention relates to the use of the antibodies and antibody fragments of the invention in prophylaxis and treatment of ZIKV infection.

Methods for the rapid detection of the presence or absence of BK virus in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting BK virus and kits are provided that are designed for the detection of BK virus.

The present disclosure provides methods and compositions for determining whether a patient exhibiting acute gastroenteritis will benefit from treatment with therapeutic agents that inhibit Norovirus genogroup I (GI) or Norovirus genogroup II (GII). The methods disclosed herein are based on detecting Norovirus genogroup I (GI) and Norovirus genogroup II (GII) in a stool sample without extracting viral nucleic acids from a clinical specimen prior to performing real-time reverse transcription PCR. Kits for use in practicing the methods are also provided.

The present invention relates to killed/inactivated and/or recombinant Senecavirus A immunogenic compositions and vaccines, and methods of preventing or treating animals in need of with such an immunogenic compositions and vaccines.

A method includes attaching two or more beads to each unit of one or more units of a chemical component in a sample, to form, for each unit of the chemical component, a multi-bead complex including two or more beads and the unit of the chemical component; placing the sample on a surface of an image sensor; at the image sensor, receiving light originating at a light source, the received light including light reflected by, refracted by, or transmitted through the beads of the multi-bead complexes; at the image sensor, capturing one or more images of the sample from the received light; and identifying, in at least one of the images of the sample, separate multi-bead complexes, the identifying of the separate multi-bead complexes including associating the two or more beads of each of the multi-bead complexes based on proximity to one another.

The present disclosure is directed to antibodies binding to and neutralizing Chikungunya virus (CHIKV) and methods for use thereof.

This invention describes a novel method for identification of known and unknown viruses from various biological and non biological samples using carrier suitable for immobilisation of membrane proteins of different target cells of human, animal or bacterial origin for capturing the virus, whose peptides are identified by mass spectrometry analysis, a diagnostic kit based on the said method and the use thereof.

A two-dimensional multiplex chromatography test card is disclosed. The two-dimensional multiplex chromatography test card comprises a first absorbent pad (11), a first nitrocellulose membrane (10), a sample pad (1), a second nitrocellulose membrane (3) and a second absorbent pad (4) which are sequentially provided on a backing (5) in a lapping manner along a length direction of the backing (5). A first conjugating zone (9) on the sample pad (1) is coated with at least one first latex bead labeled antibody, and a second conjugating zone (2) on the sample pad (1) is coated with at least one second latex bead labeled antibody. The first nitrocellulose membrane (10) is provided with a first test line (12 and/or 13) and a first control line (14), and the first test line (12 and/or 13) is coated with a first antibody corresponding to the first latex bead labeled antibody. The second nitrocellulose membrane (3) is provided with a second test line (6 and/or 7) and a second control line (8).

The present disclosure relates to methods, compositions and systems for modulation of a coronavirus (e.g., SARS CoV-2) and/or the evaluation of compounds that can modulate infectivity of the coronavirus. For example, disclosed is a method to identify a compound that can modulate infection by the coronavirus comprising the steps of: expressing an angiotensin-converting enzyme 2 receptor (ACE-2) and/or a nicotinic acetylcholine receptor (nAChR) receptor on a target cell; contacting the target cell with a viral particle comprising at least a portion of the coronavirus spike protein in the absence and the presence of the compound; and measuring levels of infection of the target cell in order to determine infectivity of the viral particle in the presence or absence of the compound. Also, disclosed are compounds and methods of treatment identified using the methods, compositions, and systems.

Compositions, kits and methods for diagnosing and treating an increased risk of moderate to severe COVID-19 disease involves a composition comprising at least one reagent capable of detecting, binding, specifically complexing with, or measuring the level of one of a subject marker in a sample. Optionally, a composition of the invention further comprises a reagent capable of detecting, binding, specifically complexing with, or measuring the expression of a subject biomarker.