Described herein is an assay for determining immune status in a subject. The assay comprises a probe for measuring disease-specific antibodies in a sample from the subject to determine a unit score. The unit score correlates to the immune status of the subject. Methods for determining the immune status of the subject are also described.

The present invention relates to a method for detecting a pathogen in cellular lysate by measuring pathogen-specific enzyme activity. The method comprises contacting the cellular lysate with a substrate the pathogen of interest recognizes and modifies, and obtaining a measurable, recordable, signal. The method may comprise detection of SARS-CoV viruses using the activity of SARS PLpro enzyme in tongue scrape lysate as a readout.

An object is to strongly suppress a false negative reaction and a false positive reaction, which cannot be sufficiently suppressed by a conventional method, in detection of an antigen such as a virus, a bacterium, or a protein to be detected in a specimen originated from a body fluid such as a nasal swab specimen, a nasal aspirate specimen, a nasal wash specimen, a blown snot specimen, a pharyngeal swab specimen, or a saliva specimen with a detection reagent utilizing an antigen-antibody reaction or a reaction between substances interacting with each other. The present invention provides a test reagent for detecting a target substance in a specimen by utilizing an antigen-antibody reaction or a binding reaction between substances interacting with each other, comprising a specimen extracting solution containing a chelating agent.

A sensor is provided for converting chemical and/or biochemical information about an analyte in a sample into an electrical signal. The sensor includes a test cantilever having a base and a deformable part of which a receptor layer is applied thereon for selective reception of an analyte of the sample. Moreover, on the base a passive test transducer is arranged and on the deformable part an active test transducer is arranged. The sensor further includes a reference cantilever having a base and a deformable part on which a reference layer is applied for selective non-reception of the analyte. Moreover, on the base a passive reference transducer is arranged and on the deformable part an active reference transducer is arranged.

Devices, methods and test kits for the detection of coronavirus infection are disclosed. The methods include detecting the presence of coronavirus antigens comprising the use of antibodies bound to nanoparticles immobilized on a solid support chromatographic immunoassay. The devices, methods and test kits include detection of SARS-CoV-2.

An embodiment provides a method for detection of the COVID-19 virus, including: obtaining a body fluid from a patient; exposing the body fluid to at least one binding antibody, wherein the at least one binding antibody binds to an antigen in the body fluid; forming an antigen antibody complex; and determining the presence of the antigen antibody complex. Other aspects are described and claimed.

Provided herein are binding proteins recognizing HPV16 E7 antigen and uses thereof.

Disclosed herein are plant-based, molecular and diagnostic tools that can be used to block aphid transmission of poleroviruses, including stabilized proteins for expression in transgenic plants and/or in formulations for direct plant delivery. Further disclosed are proteins that can kill aphids and methods to produce these proteins. Antibodies and useful for diagnostic and therapeutic uses against poleroviruses.

Methods of determining infection type are disclosed. In one embodiment, the method comprises measuring the amount of TRAIL and/or IP10 no more than two days from symptom onset.

Triazabutadiene molecules as cleavable cross-linkers adapted to cross-link components with click chemistry, e.g., clickable triazabutadienes. For example, in some embodiments, the triazabutadienes feature alkyne handles attached to the imidazole portion or the aryl portion of the triazabutadienes, wherein the alkyne handles can link to azide handles (e.g., azide handles disposed on other components) via click chemistry. Also described are methods of producing said clickable triazabutadienes and methods of use of said clickable triazabutadienes. The present invention also features methods of cleaving said clickable triazabutadienes, e.g., for liberating the diazonium species for further chemical reactions.