The present invention relates to vaccines and therapeutic compositions targeted at coronaviruses, specifically, the Severe Acute Respiratory Syndrome coronavirus 2 (SARS CoV2). More specifically, the invention provides reconstituted Receptor Binding Motif (RBM) of a Spike protein of the SARS CoV2, Receptor Binding domains (RBDs), and spike proteins comprising the reconstituted RBMs or τ-linkers, compositions, vaccines, therapeutic and diagnostic methods and uses thereof.

The Rubella Virus Spike construct comprises at least one E1 component and one E2 component, which are linked together. The E1 component consists of the El envelope protein, whose C-terminal transmembrane region and intravirional domain are removed and whose N-terminus comprises the ectodomain of the El envelope protein. The E2 component consists of the E2 envelope protein whose transmembrane regions and intravirional domain removed and whose C-terminus comprising the ectodomain of the E2 envelope protein. The C-terminus of the E2 component is connected to the N-terminus of the E1 component by direct fusion or by means of a linker to form an E1-E2 fusion protein.

One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps: (a) binding the antigen of interest to beads made of protein-binding polymers (b) incubating the microorganisms of the expression library with the pearls of stage (a), (c) placing the beads of step (b) in a tube with an inert medium with a density greater than or equal to 1 g/mL and centrifuge for 45 s to 2 minutes at a rate between 150 and 250 g; d) discarding the upper fraction and supernatant with the free microorganisms, and obtain the beads with the linked microorganisms, which correspond to those that express HHV that recognizes the antigen.

The embodiments disclose a method including functionalizing a biosensor with a biologic analytical target prior to installation into a detection cartridge, depositing a test subject bodily fluid test sample onto the biosensor surface, inserting the detection cartridge into a portable detection cartridge reader, measuring the electrical impedance of the bodily fluid test sample across biosensor energized electrodes, providing algorithms for analyzing measured electrical impedance data of the bodily fluid test sample obtained in the detection cartridge, identifying and determining the presence of biologic analytical target molecules in the bodily fluid test sample, and transmitting results of the test results to the test subject.

The present invention relates to the use of a condensate collector such as portable or stationary dehumidifier placed in a defined testing space or area and used as a readily available and affordable tool to collect airborne virus particles in collected condensate from the testing atmosphere in the defined testing space or area, wherein the collected condensate is analyzed for virus biomarkers to identify viruses, such as SARS-CoV-2 or mutants or variants thereof, in the testing atmosphere.

The present disclosure provides methods for the detection of target specific antibodies in samples. The methods include the detection of pathogen-specific antibodies, such as SARS-CoV-2 specific antibodies. Also included is a kit for use for the detection of pathogen specific antibodies in a sample. In one aspect, the disclosure provides a method of detecting the presence of a target specific antibody in a sample by (i) contacting the sample with a test cell comprising one or more exogenous nucleic acid sequences encoding one or more target proteins; and (ii) detecting the presence of the target specific antibody in the sample by contacting the immune complex of (i) with an anti-immunoglobulin (Ig) antibody, and detecting the anti-immunoglobulin (Ig) antibody, thereby detecting the presence of a target specific antibody.

The present disclosure relates to a specimen collection solution for a one-step rapid antigen diagnostic test. More specifically, the present disclosure relates to a specimen collection solution including soybean-derived terminally truncated canavalin, sodium chloride, a surfactant, and an emulsifier. Moreover, the present disclosure relates to a diagnostic kit and diagnostic method for a one-step rapid antigen diagnostic test including the specimen collection solution.

A colorimetric sensor for detecting bacteria and/or includes one or more layers having a photonic crystal structure a functional layer comprising a nanomaterial capable of generating bacteria- and/or viruses-bioresponsive surface plasmon overlapping the one or more layers having the photonic crystal structure. The bacteria- and/or viruses-bioresponsive nanomaterial of the functional layer is doped with proteinic substances or antibodies acting as virus receptors, or the colorimetric sensor comprises a receptor layer comprising proteinic substances or antibodies acting as virus receptors. The functional layer and receptor layer overlap each other. Alternatively, or in addition to, the colorimetric sensor comprises a plasmonic nanostructured layer comprising nanostructures such to generate plasmonic colors, overlapping the one or more layers having the photonic crystal structure.

Provided herein are diagnostic methods, devices and kits for detecting neutralizing antibodies to SARS-CoV-2.

The instant disclosure discloses an antibody or antigen-binding fragment thereof binding to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and uses of such antibody or antigen-binding fragment thereof to create immunoassay methods or devices for PRRSV detection.