A peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus is provided. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 269.

Provided herein are affinity agents comprising ligands that specifically bind adeno-associated virus. The affinity agents are useful for binding, isolation, and/or purification of adeno-associated virus. Further disclosed are amino acid sequences of binding motifs or polypeptides comprised by the ligands, and associated modifications of the binding motifs and/or polypeptides, as well as a method of making the affinity agents.

Methods for detecting an immune response to Mycobacterium tuberculosis are provided. Aspects of the methods include contacting a biological sample comprising T cells from a subject with at least one M. tuberculosis peptide and determining the presence of T cells in the biological sample that specifically recognize the amino acid sequence of the at least one peptide, wherein the presence of T cells that specifically recognize the amino acid sequence of the at least one peptide indicates the subject has an immune response to M. tuberculosis. In certain aspects, the methods of the present disclosure may include treating a subject identified as having an immune response to M. tuberculosis. Also provided are a skin test and immunoassay kits for practicing the disclosed methods.

Provided are a vector, a recombinant virus, and a method of using and making thereof. Also provided are immunological compositions containing the recombinant African swine fever virus (ASFV) for inducing an immunological response in a host animal to which the immunological composition is administered. Further provided is a kit and a method of detecting the presence of ASFV immunogens in a sample from an animal.

The present disclosure provides, in one aspect, a target substance detection method in which a trapping substance that indirectly binds to both an immobilization side (support side) and a detection side (reporter side), which is a method for detecting a target substance in a sample. The method includes a reaction step of reacting the sample, a support including a first binding portion, a reporter substance including a second binding portion, a first trapping substance that includes a first binding partner portion capable of binding to the first binding portion and that can bind to the target substance, and a second trapping substance that includes a second binding partner portion capable of binding to the second binding portion and that can bind to the target substance; and a detection step of detecting a signal from the reporter substance.

The present disclosure discloses a protein-based multifunctional molecular switch for antibody detection, and belongs to the field of protein detection. The molecular switch is a fusion protein including the following parts ligated sequentially from an N-terminus to a C-terminus: a part (1): SmBiT; a part (2): an epitope polypeptide capable of being specifically bound by the antibody to be detected; and a part (3): LgBiT. In the present disclosure, the molecular switch can be specifically recognized by an antibody through the epitope polypeptide, thereby affecting binding of the SmBiT and the LgBiT, and greatly changing a luciferase activity before and after to reflect a concentration level of the antibody. The molecular switch can be used to detect a 2019 novel coronavirus (SARS-CoV-2) with an accuracy and specificity close to 100%, which has an extremely desirable use value.

A method for detecting MERS-CoV at high sensitivity and specificity using IgY antibodies that bind to MERS-CoV N protein, its fragments and domains. Isolated or purified IgY monospecific antibodies to MERS-CoV N protein.

The application relates to certain new epitope peptides associated with coronavirus envolop protein and the use thereof. In particular, the application describes the antibodies and vaccines developed against these peptides, and the use thereof for the detection and treatment of coronaviral infection in human subjects.

A lateral flow assay strip and a molecular diagnostic methods are disclosed. The lateral flow assay strip includes a sample pad (100), a conjugate pad (200), a test pad (300), a control pad (400), and an absorption pad (500). The sample pad (100) receives a sample containing at least one among amplicons each labelled with at least one tag and amplicon precursors each labelled with a tag. The conjugate pad (200) includes a first conjugate (C1) attached in a flowable manner and having an indicator and a detector bound to the indicator. The test pad (300) includes a test line with a first trapping molecule fixed. The control pad (400) includes a control line with a second trapping molecule fixed. The lateral flow assay strip is used to detect amplicons such as nucleic acids amplified by using a primer or an amplicon precursor such as dNTP, on which one type of tag is labelled.

Described herein are diagnostic and control fusion protein reagents and methods for use thereof in simple rapid and inexpensive hemagglutinin assays for the detection of subject antibodies directed to the SARS-CoV-2 virus.