There are provided methods for quantifying an analyte in a sample, diagnosing a condition characterized by an excess or a depletion of an analyte in a biological sample, isolating analyte from a sample, and detecting an analyte in a sample. These method comprise the steps of providing a colloidal suspension of nanoparticles of a plasmonic metal, the nanoparticles having attached on their surface a binding moiety for selective attachment of said analyte and adding the sample to the suspension, thus producing a mixture in which said analyte is attached to the nanoparticles in suspension. Then, the methods further comprise the steps of either allowing sedimentation of the nanoparticles with bound analyte, thereby producing a sediment comprising the nanoparticles with bound analyte and a supernatant, and measuring the Localized Surface Plasmon Resonance (LSPR) spectrum of the supernatant and/or recovering the sediment, or measuring the Localized Surface Plasmon Resonance (LSPR) spectrum of the mixture.

Provided are: a coloring cellulose microparticle enabling false positive to be significantly reduced while maintaining a high detection sensitivity; and an immunochromatographic diagnostic kit using the same.

The present invention provides the use of a lead (IV) containing compound to prepare a lead chalcogenide nanocrystal and a method for producing broadband lead chalcogenide nanocrystals in a low cost, size-controllable and scalable method, the method comprising contacting a lead (IV) containing compound with an organic acid and a chalcogen-containing reagent.

Methods for the detection of enzymatic activity, in particular, to in vivo methods. A non-invasive method for in vivo enzyme activity detection, such as activity of proteinases, to substrates specifically developed for these methods and to a device detecting product formation of the enzyme to be tested based on determination of signals produced by the substrates and/or its products.

Lateral flow immunoassay devices, systems, methods, and kits described herein identify, measure, detect, and analyze analytes of interest in a sample. The lateral flow' immunoassay devices described herein include a test strip without additional features common to traditional lateral flow7 immunoassays, such as housing, pads, or other materials that require complex manufacturing equipment and protocols. Thus, the devices, systems, methods, and kits described herein relate to simplified devices that eliminate complex and expensive manufacture equipment and methods.

The present disclosure relates to a kit for quantitative detection using a fluorescent microarray, and belongs to the technical field of protein detection. The kit of the present disclosure includes a detection plate and a detection antibody coupled with fluorescent microspheres, where the detection plate is provided with a plurality of reaction chambers; the reaction chamber is provided with an opening, and an inner bottom surface of the reaction chamber is provided with a plurality of detection sites that are arranged side by side along a length direction of the reaction chamber at an interval. The kit of the present disclosure may detect allergen-specific IgE, IgG and IgA with high sensitivity, as well as rapidly and quantitatively detect an allergen-specific antibody IgE, IgG and IgA concentration in human serum or plasma, and may screen dozens of allergens at a time.

Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

The present invention relates to a standard material composition for verifying a bio-analysis equipment, comprising quantum dot-containing nanoparticles. Through a standard strip and/or a standard tray, which are made of the standard material composition, the present invention may increase the analysis accuracy of a bio-analysis equipment.

The present disclosure relates to luminescent including photon up-conversion nanoparticles. These nanoparticles include a polymeric organic matrix, at least one light emitter distributed within this matrix, a stabilizing agent, and at least one metal particle enclosed within the matrix, wherein the metal particles are plasmonic nanoparticles. The present disclosure further relates to methods of manufacture and to uses of such nanoparticles.

The present disclosure relates to a novel method for lateral flow immunoassay (LFIA) by utilizing plasmonic enhancement strategy. More specifically, the present disclosure provides a plasmonic enhanced lateral flow sensor (pLFS) concept by introducing a liposome-based amplification of the colorimetric signals on the lateral flow platform for ultrasensitive detection of pathogens.