A method for screening targets by data independent acquisition (DIA)-based quantitative chemical proteomics, comprising covalently modifying specific active amino acids in a proteome with an active molecular probe; and quantitatively analyzing sites covalently-modified by the probe through DIA-based quantitative omics method, to obtain candidate targets. According to the method for screening targets by DIA-based quantitative chemical proteomics, DIA-based quantitative omics technique is applied into activity-based protein profiling (ABPP) to form DIA-ABPP, so that high coverage, high reproducibility and high precision target screening may be achieved, and corresponding technical support is provided for subsequent drug development.

In aspects, a method includes: dividing a sample including a mixture of polypeptides into at least two factions that include a starting fraction and at least one other fraction; contacting polypeptides in each of the other fraction(s) with at least one agent for cleavage of the polypeptides, to provide at least one fragmented fraction; analyzing polypeptides in the starting fraction and polypeptides in the fragmented fraction(s) to provide starting sequences for the starting fraction and fragment sequences for the fragmented fraction(s); grouping the starting sequences and the fragment sequences into at least one cluster, where each of the cluster(s) include at least one starting sequence and at least one fragment sequence; and for each cluster, generating a computed sequence, based on values in the at least one starting sequence and values in the at least one fragment sequence of the respective cluster, for a polypeptide in the sample.

In one aspect the disclosure relations to means and methods for identifying a protein or a DNA encoding the protein, involved in the production of a product by a micro-organism. In the methods the micro-organism is cultured under different culture conditions each of which exhibit a different level of the product that is produced by the micro-organism. The genetic expression of the genes of the micro-organism is compared with the level of the product, and groups of DNAs are identified that are involved in the production of the product by the micro-organism.

The disclosure provides materials and methods for permanently labeling cells by transducing cells with viruses comprising a barcode. The disclosure is based on the idea that tracking, selecting and isolating lymphocytes labeled with the barcode can facilitate identification of clonal cells responsible for antibody or antibody fragment production.

The present disclosure provides methods and systems for sequencing proteins. One or more methods disclosed herein may use linkers having an amino acid-reactive group and an additional reactive moiety that may be used to couple a polymerizable molecule. The linker may couple to a polymerizable molecule and an amino acid of a peptide and a capture moiety via the polymerizable molecule, followed by cleavage of the amino acid from the peptide. Further processing and analysis may be conducted using, for example, nanopores or nanogaps.

The present disclosure provides methods and systems for sequencing proteins. One or more methods disclosed herein may use linkers having an amino acid-reactive group and an additional reactive moiety that may be used to couple a polymerizable molecule. The linker may couple to a polymerizable molecule and an amino acid of a peptide and a capture moiety via the polymerizable molecule, followed by cleavage of the amino acid from the peptide. Further processing and analysis may be conducted using, for example, nanopores or nanogaps.

The present disclosure provides methods of treating subjects having hearing loss, methods of identifying subjects having an increased risk of developing hearing loss, and methods of detecting Kelch Domain Containing 7B (KLHDC7B) variant nucleic acid molecules and variant polypeptides.

The present invention relates to the field of biochemistry, more particularly to proteomics, more particularly to protein sequencing, even more particularly to single molecule peptide sequencing. The invention discloses means and methods for single molecule protein sequencing and/or amino add identification using cleavage inducing agent. Said cleavage inducing agents which are not specific for one particular amino acid, cleave polypeptides step by step from the N-terminus onwards and provide information on the identity of the cleaved amino acids based on the kinetics of said reaction.

The disclosure provides probes comprising dipyrromethane-BF.sub.2 derivatives which exhibits different fluorescent spectral properties when conjugated to the amnio acids, compositions and kits comprising same. The disclosure also provides methods for detecting/identifying amino acids and sequencing polypeptide molecules by conjugating a dipyrromethane-BF2 derivative which exhibits different fluorescent spectral properties when conjugated to the amnio acids.

The present invention provides a structure for scavenging a compound, the structure including at least one gene from a gene group involved in the intracellular/extracellular transport of the compound, a product of the gene, or a combination of the gene and product.