A urine sample testing apparatus may include a urine qualitative measuring section configured to acquire a measurement result for each of a plurality of urine qualitative measurement items and a urine sediment measuring section configured to acquire a measurement result for each of a plurality of urine sediment measurement items. The apparatus may also include an operation part that can specify a combination of one of the plurality of urine qualitative measurement items and one of the plurality of urine sediment measurement items. An information processing unit may be included and configured to determine whether a first measurement result of the urine sample obtained by the urine qualitative measuring section and a second measurement result of the urine sample obtained by the urine sediment measuring section have a predetermined relationship with respect to the urine qualitative measurement item and the urine sediment measurement item included in the specified combination.

The present disclosure describes a method of quantifying amounts of phosphopeptides using isotopically-enriched peptides as internal standards. A kit comprising at least one isotopically-enriched phosphorylated peptide can be used to quantify changes in amounts of phosphopeptides using parallel reaction monitoring mass spectrometry techniques. The invention can be used to indicate the pathologic mechanism, severity of the disease, and treatment response of a subject. The invention can also be used to identify subjects who require more aggressive therapeutic interventions or alternative treatments.

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

The invention relates to a method for normalization of a label-free system for calibration-free concentration analysis. The method comprises (1) providing a solution containing a control macromolecular particle of a known concentration at a pH lower than the pI of the macromolecular particle and a low ionic strength; (2) contacting the solution with a negatively charged optical sensor surface at a first flow rate to allow electrostatic binding of the macromolecular particle to the surface and obtaining a first sensorgram; (3) contacting the solution with the optical sensor surface at a second flow rate to allow electrostatic binding of the macromolecular particle to the surface and obtaining a second sensorgram; and (4) fitting the sensorgrams to a binding equation to determine a measured concentration of the control; wherein the optical sensor surface is not immobilized with a ligand for the control and the contacting steps are performed under mass transport limitations. Also provided is a kit for performing the method, as well as a method for determining a concentration of an analyte.

A pallet comprising a primary body having an upper deck, a lower deck, and a set of lift channels. The upper deck includes horizontally extending upper surfaces and downwardly sloping walls extending from the upper surfaces. The horizontally extending upper surfaces are configured to receive flat objects. The downwardly sloping walls form upper support cradles for receiving horizontally-oriented curved objects thereon. The lower deck includes horizontally extending lower surfaces and upwardly sloping walls extending from the lower surfaces. The horizontally extending lower surfaces are configured to be positioned on flat objects. The upwardly sloping walls form lower support cradles for positioning the pallet on horizontally extending curved objects. The lift channels are vertically spaced from the upper support cradles and the lower support cradles such that the curved objects positioned above and below the pallet will not interfere with lifting forks being inserted into the lift channels.

Provided herein are compositions and methods for proteomic analysis. In particular, provided herein are compositions and methods for performing mass spectrometry-based protein quantitation analysis.

The present invention concerns a method for determining the concentration of a protein in a gastrointestinal (GI) tract sample taken from a human or an animal. The present invention is characterized by the feature that a dilution of the sample in the buffered aqueous extraction medium in a range of 1:100 to 1:10,000 is obtained. The present invention leads to a significant improvement of the technical situation, and provides a simple, sensitive and specific determination tool of proteins in GI tract samples. The determination of proteins, e.g. calprotectin, elastases or hemoglobin, in GI tract samples leads to more accurate and reproducible results.

A method for obtaining information about proteinuria and/or nephropathy from urine samples is provided for evaluating a urine sample that includes detecting proteins in the urine sample with two types of detection reagents that differ in reactivity to at least one urinary protein; and based on an indicator calculated using the results of the detection with the two types of detection reagents.

Provided are methods for the detection or quantitation of amyloid beta. In a particular aspect, provided herein are methods for detecting amyloid beta or fragments thereof by mass spectrometry. In another aspect, provided herein are methods for determining the ratio of amyloid beta 42 (A42) to amyloid beta 40 (A40). In another aspect, provided herein are methods for diagnosis or prognosis of Alzheimer's disease or dementia.

The present invention provides a method for detection of a basic peptide by mixing a sample suspected to contain the basic peptide and a reagent containing denatured albumin and detecting turbidness due to a complex of the basic peptide and denatured albumin.