This disclosure relates to a method of generating conditionally active biologic proteins from wild type proteins, in particular therapeutic proteins, which are reversibly or irreversibly inactivated at the wild type normal physiological conditions. For example, evolved proteins are virtually inactive at body temperature, but are active at lower temperatures.

The present invention is related to a method for recovering two or more genes, or gene products, or cDNAs, encoding for an immunoreceptor having two or more subunits, which two or more genes, or gene products, are comprised in a given source cell. The invention is further related to a method of creating a library of expressor cells, in which library each cell is capable of expressing two or more genes, or gene products, encoding for the subunits of the immunoreceptor. The invention is further related to a method of screening a library of expressor cells as created according to the above method, for one cell that expresses an immunoreceptor that has specificity for a given target molecule.

Fibronectin type III (.sup.10Fn3) binding domains having novel designs that are associated with reduced immunogenicity are provided. The application describes alternative .sup.10Fn3 binding domains in which certain immunogenic regions are not modified when producing a binder in order to maintain recognition as a self antigen by the host organism. The application also describes .sup.10Fn3 binding domains in which HLA anchor regions have been destroyed thereby reducing the immunogenic contribution of the adjoining region. Also provided are .sup.10Fn3 domains having novel combinations of modified regions that can bind to a desired target with high affinity.

Disclosed herein are compositions comprising a compound of Formula (I) and methods for treating or prophylaxis of porcine reproductive and respiratory syndrome (PRRS) therewith (I).

##STR00001##

Provided herein are methods for identifying modulators of cell surface protein interactions and activities, as well as modulators of cell surface protein interactions and activities.

The present invention provides methods and systems to identify host cell protein (HCP) impurities in a sample containing high-abundance proteins. The HCP impurities can be enriched using interacting peptide ligands which have been attached to solid support. The HCP impurities can be eluted from the solid support. The isolated HCP impurities can be subjected to limited digestion to generate components of the isolated HCP impurities which can subsequently be identified using a mass spectrometer. The present invention also provides methods and systems to identify sequence variant (SV) peptides or proteins in a sample containing high-abundance proteins. The SV peptides or proteins can be enriched using interacting peptide ligands which have been attached to solid support. The SV peptides or proteins can be eluted from the solid support. The isolated SV peptides or proteins can be subjected to full or limited digestion to generate components of the isolated SV peptides or proteins which can subsequently be identified using a mass spectrometer.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention relates to the use of a particle, including a virus-like particle (VLP), for the discovery and analysis of protein-protein interactions that are modulated by small molecules.

This disclosure relates to a virus-like particle in which a small molecule-protein complex is entrapped, ensuring the formation of the small molecule-protein complex under physiological conditions, while protecting the small molecule-protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of small molecule-protein complexes.

Polypeptides with biophysical properties such as solubility, stability, high expression, monomericity, binding specificity or non-aggregation, including monomeric human heavy and light chain variable domains (V.sub.Hs and V.sub.Ls), are identified using a high throughput method for screening polypeptides, comprising the steps of obtaining a phage display library, allowing infection of a bacterial lawn by the library phage, and identifying phage which form larger than average plaques on the bacterial lawn. Sequences of monomeric human V.sub.Hs and V.sub.Ls are identified, which may be useful for immunotherapy or as diagnostic agents. Multimer complexes of human V.sub.Hs and V.sub.Ls are also identified. The V.sub.Hs and V.sub.Ls identified may be used to create further libraries for identifying additional polypeptides. Further, the V.sub.Hs and V.sub.Ls may be subjected to DNA shuffling to select for improved biophysical properties.