The invention relates to a method for identifying and quantifying a polypeptide from a library of polypeptides. The method comprises the steps of: 1providing a polypeptide library and a detection tag library, 2generating a nested library comprising the polypeptides and the detection tags, 3sequencing the nested library, 4selecting a member of the nested library in one or several selection steps that are independent of a physical genotype-phenotype linkage, 5isolating the detection tag from the selected polypeptide, 6identifying and quantifying the detection tag by mass spectrometry, 7obtaining the sequence of the selected polypeptide. The invention also relates to a collection of polypeptides, a collection of detection tags, and a collection of plasmid vectors.

This invention provides a device for screening a candidate substance for an active ingredient to prevent or treat declined kidney function etc. A screening device 1 for screening a candidate substance for an active ingredient to prevent or treat declined kidney function etc. comprises a first measurement value obtaining unit 11 for obtaining a measurement value of a kidney function prediction marker protein and/or a measurement value of mRNA of the protein in a test-substance-treated specimen, and a candidate substance determination unit 14 for determining that the test substance is a candidate substance for the active ingredient based on the measurement value(s) obtained by the first measurement value obtaining unit 11.

Systems and methods for obtaining qualitative or quantitative measurements of proteoforms of polypeptides are described. The described methods include measurements of affinity reagent binding on single-molecule polypeptide arrays to distinguish between polypeptide isoforms. The described methods may provide high resolution quantitative comparisons of proteoforms with very low copy numbers.

Provided herein are methods of identifying an inhibitory activity of an exogeneous agent that disrupts binding of a target peptide to a biomolecule using phase separation as in cellulo read-out. Provided herein also are kits for the methods, pharmaceutical compositions comprising the agents screened by the methods, methods of preparing the pharmaceutical composition, and methods of treating a disease or condition in a subject in need thereof by administering the pharmaceutical composition.

A method for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding is disclosed.

A method for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding is disclosed.

The present invention relates to a method for identifying a candidate therapeutic for a disease caused by infection with a human cytomegalovirus (HCMV) having a glycoprotein B (gB) polypeptide, comprising contacting the HCMV gB polypeptide comprising a druggable region with a compound, wherein binding of said compound indicates a candidate therapeutic. The present invention also relates to candidate therapeutics comprising modulators and inhibitors of HCMV activity and pharmaceutical compositions comprising said modulators and inhibitors and methods of use thereof.

The invention relates generally to the field of biochemistry. In particular, the invention relates to a method of detecting or measuring target that is bound to a ligand in a sample, the method comprising contacting a sample comprising one or more cells with a cell-permeable denaturant to promote intracellular unfolding of the target in the sample, followed by lysing the sample. The lysed sample is then detected or measured for the level of non-aggregated target or aggregated target, wherein a difference in the level of non-aggregated target or aggregated target as compared to a reference indicates the presence or level of target that is bound to the ligand in the sample. In specific embodiments, the cell-permeable denaturant is urea or derivatives thereof. Methods of identifying a candidate ligand or predicting the efficacy of a drug in a subject are also provided therein.

Provided herein is a platform technology for designing stabilized peptides that covalently bind their target protein and thereby inhibit the activity of the target protein. Also provided are exemplary stabilized peptides that can be used for covalent modification of their target proteins.

In a method for analyzing cells, a sample fluid having a suspending medium and cells is fed to a microfluidic device having at least one cell processing unit having a trapping region, a reaction unit, and an outlet arrangement. The trapping region is delimited by at least an input valve and a sieve valve, in particular a v-type valve that is capable of retaining the cells while letting fluids pass. The method includes trapping cells in the trapping region, subsequently establishing a flow of a reaction fluid through the trapping region while the sieve valve assumes the open state, such that the reaction fluid transfers the trapped cells from the trapping region into the reaction unit, decomposing the transferred cells into cell fragments through a decomposition process, collecting the cell fragments in the outlet arrangement, and analyzing the cell fragments.