An imaging mass spectrometer according to one aspect of the present invention includes an analysis executing section (1) configured to collect data by executing predetermined analysis on each of a plurality of micro regions set in a two-dimensional measurement region (50) on a sample (50) or a three-dimensional measurement region in the sample; a first image creating section (21) that uses the data obtained by the analysis executing section (1) to create one or a plurality of first distribution images each reflecting a distribution of one or a plurality of specific components included in the sample (50); a formula storage section (23) that stores, as a formula, a chemical reaction formula including at least the one or a plurality of specific components as elements, or a calculation formula including an amount of the specific component as element; a signal value calculating section (25) that calculates different signal values from the signal values in the micro regions constituting the one or the plurality of first distribution images by using the formula acquired from the formula storage section (23) in response to a user's instruction; and a second image creating section (26) that creates a second distribution image based on a calculation result.

A method of mass spectrometry for analyzing a sample within a mass range of interest includes the steps: ionizing the sample to produce a plurality of precursor ions; performing an MS1 scan of the precursor ions comprising mass analyzing the precursor ions across the mass range of interest, to obtain an MS1 mass spectrum of the precursor ions; determining ion intensity values within the MS1 mass spectrum; selecting precursor mass segments within the mass range of interest, and for each precursor mass segment: fragmenting the precursor ions within that precursor mass segment; and performing an MS2 scan of the fragmented ions by: controlling an amount of fragmented ions for that precursor mass segment, based on an intensity value for that precursor mass segment derived from the MS1 spectrum; and mass analyzing the amount of fragmented ions.

Packed-tip electrospray ionization (ESI) emitters for mass spectrometry are described. In one aspect an ESI emitter stores a first type of particle. A liquid chromatography (LC) column is coupled with the emitter via a junction. The LC column stores a different type of particle than the ESI emitter to facilitate better chromatographic and ESI performance.

The present invention relates to methods for simultaneously identifying and/or detecting distinct classes of biomarker in biofluid samples, such as blood.

The present disclosure generally pertains to methods of identifying and quantitating host cell proteins (HCPs) in therapeutic protein development. In particular, the present invention generally pertains to methods of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for unbiased identification and sensitive quantitation of HCPs in therapeutic protein development.

Disclosed are a deer-derived specific peptide and a detection method therefor; by screening through a large number of experiments, a ratio of relative contents of two deer-derived peptides is determined, and a graph is drawn by using a proportion of a deer antler gelatin in a mixed gelatin as an abscissa and using a value of A.sub.peptide .sub.1/A.sub.peptide .sub.2 as an ordinate; the proportion of the deer antler gelatin is linear with A.sub.peptide .sub.1/A.sub.peptide .sub.2 as a standard curve equation to distinguish a deer hide gelatin from the deer antler gelatin; the method can be used for distinguishing the deer antler gelatin from the deer hide gelatin, and controlling the quality; a defect in the prior art that the deer antler gelatin and the deer hide gelatin are difficult to distinguish in appearance, and are also difficult to distinguish by using a specific peptide fragment, is solved.

Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system. The methods, systems, and kits described herein provide for substantially increased speed/throughput and ease of use for a wide range analytes with essentially no compromise in specificity for individual analytes relative to previously described methods.

The invention provides mass spectrometry methods, compositions and systems which enable a unique platform for analyte quantitation accessing very high degrees of multiplexing and accurate quantification, particularly well-suited for a range of quantitative analysis for proteomics applications. Embodiments of the present methods and systems combine isotopic coding agents characterized by very small differences in molecular mass with mass spectrometry methods providing large resolving power to provide relative or absolute analyte quantification in a large number of samples.

The present disclosure relates to a method of isolating extracellular vesicles directly from human tissues. The invention further relates to a method of identifying disease and tissue specific membrane proteins on extracellular vesicles by membrane isolation and proteomic analysis. The invention further relates to methods of diagnosing diseases by capturing extracellular vesicles by the use of disease specific membrane proteins from body fluids, and detecting or analyzing molecular signatures (proteome, DNA, and RNA) on captured extracellular vesicles. Moreover, the present invention relates to kits, apparatus and software required for implementing aforementioned methods.

The present invention generally pertains to methods of characterizing peptides or proteins of interest. In particular, the present invention pertains to the use of the nonionic surfactant DDM to improve recovery of hydrophobic peptides in pharmacokinetic, reduced peptide mapping, or other biotherapeutic protein analyses.