A method for disrupting a sample of biological material of human, animal, or plant origin for subsequent proteome analysis by a mass spectrometry method is provided. The method involves disrupting the sample by treatment with a certain volume of an organic acid until the sample completely dissolves, incubating the sample for a certain period, and then neutralizing the sample with a neutralizing solution until a pH value between 7 and 9 is reached.

A system, method, diagnostic and container delivery system for manipulating a target, by manipulating with the quantum coherence of the target. The method includes identifying intrinsic parameters of the target and determining target-tuned design factors based at least partially on the intrinsic parameters. Target-tuned electrons and fields are generated based in part on the target-tuned design factor. The target-tuned electrons and fields are defined by discrete quantized energy levels. The method may include preparing a container to carry the unquantized target-tuned electrons, the container being composed of superconductor quantum dots. The unquantized target-tuned electrons are transferred to the container to form target-tuned artificial atoms having quantized target-tuned electrons, which may be delivered to the target as a manipulating agent. Alternatively, the unquantized target-tuned electrons may be delivered directly to the subject.

An apparatus and method for detecting an analyte of interest using paper spray mass spectrometry includes a spray material; a sample on the spray material including an enzyme of interest; a solvent to hydrate the sample, promote enzymatic activity, and extract analytes of interest from the sample; a substrate specific to the enzyme of interest, wherein any of the spray material and the solvent includes the substrate; a voltage source to apply a voltage to the spray material to create charged droplets of a mixture containing the sample; and a mass spectrometer to perform spectrometry on the droplets to perform any of: identify the analytes of interest in the sample; and measure a level of inhibition in any enzymes contained in the sample.

The identification of new biomarkers that are specifically associated with skin dryness. These biomarkers, which are extended synaptotagmin-3 (ESYT3), Ubiquinin-carboxyl terminal hydrolase 7 (USP7), periplakine (PPL) and Late Cornified Envelop Protein 1C (LCE1C), allow for the accurate evaluation of the moisture content of the skin, and particularly allow for the diagnosis of dry skin. Also, a method for determining the moisture content of the skin, and particularly for diagnosing dry skin in a subject, the method including a step of measuring, in a skin sample obtained from the subject, the expression level of at least one protein selected from ESYT3, USP7, PPL and LCE1C. Further, a method for evaluating the efficacy of a cosmetic treatment for hydrating the skin and to a method for screening candidate cosmetic compounds, both being based on measuring the expression level of the above biomarkers.

A composite magnetic nanomaterial based on a DNA tetrahedron, preparation therefor and use thereof are provided. The preparation method include the steps of synthesizing a DNA tetrahedron by means of a self-assembly reaction of single-stranded DNA chains, loading magnetic nanoparticles on the surfaces of molybdenum disulfide particles, modifying gold nanoparticles on exposed active sulfur atoms of the molybdenum disulfide particles, modifying the DNA tetrahedron on the gold nanoparticles, and bonding a protein antibody on the DNA tetrahedron. The composite magnetic nanomaterial is used for enriching and detecting low-abundance proteins in serum, and specific low-abundance proteins in the serum can be efficiently and selectively enriched by means of the specific reaction between an antigen and an antibody.

Methods and system for identifying and quantifying antibody fragments and identifying the site of fragmentation on an antibody are provided herein.

The present invention relates to a method, a diagnostic system, a dopand and the use thereof for the enhancement of detection of an analyte of interest by Cross Spray ESI mass spectrometry.

Provided herein are methods for detecting and identifying disease-specific biomarkers, such as target antigens associated with infection.

Methods and system for identifying and/or quantifying a protein are provided herein.

A method for screening a split site and an application thereof are provided. The method includes: S1, writing a program using a computer language, and predicting an amino acid sequence formed by connecting adjacent peptide fragments after an intein is embedded into each two adjacent amino acid residues in an initial amino acid sequence and then excised through a self-splicing reaction to construct a protein database; and S2, performing molecular clone after inserting an intein sequence into a gene segment and then translating to obtain a peptide fragment, detecting whether that peptide fragment contain a labeled amino acid sequence by mass spectrometry, and comparing the peptide fragment with the protein database to confirm the split site. A final detection is realized by the mass spectrometry instead of high-throughput screening, and extended to searches for the split site of any active protein.