The present invention provides a novel serotype determination method for E. coli and the like which is not subject to the limitations of indirect methods such as antigen-antibody reaction and gene analysis.

For example, provided is a method of determining a serotype of E. coli, comprising the following steps 1 to 3: 1. a step of selecting a first peak group consisting of a plurality of peaks which are successively and repeatedly detected at equal intervals in a spectrum obtained by performing a mass spectrometry on a sample containing an antigenic site obtained from a microbial specimen of a determination subject, and then obtaining a difference in m/z value between adjacent peaks of the first peak group as a peak interval actual measurement m/z value of the first peak group, p0 2. a step of collating the peak interval actual measurement m/z value of the first peak group with a separately obtained m/z value for the basic structure corresponding to a specific serotype, and 3. a step of determining the specific serotype corresponding to the separately obtained m/z value for the basic structure consistent with the peak interval actual measurement m/z value of the first peak group, as a serotype of the microorganism, from the result of the above-mentioned collation step.

This invention relates to graphical user-interactive displays for use in MS-based analysis of protein impurities, as well as methods and software for generating and using such. One aspect provides a user-interactive display comprising interactive and dynamic selection of one or more masses and concurrent display of peaks (points) corresponding to that predicted mass value across other displays (MS1, deconvolved mass spectrum, etc.).

A method of preparing a characterization zone of an analysis plate to carry out a characterization of a population of a microorganism in the presence of an antimicrobial agent by mass spectrometry using the MALDI technique. The method involves the following steps in succession: a step for providing an analysis plate for a characterization by means of the MALDI technique, the plate including an analysis zone carrying an antimicrobial agent; a step of depositing the population of a microorganism onto said analysis zone in contact with the antimicrobial agent; an incubation step of preserving the analysis plate under conditions and for a sufficient time to allow the antimicrobial agent and the microorganism that is present to interact; and a step of depositing a matrix that is suitable for the MALDI technique onto the analysis zone; associated characterization and functionalization methods, analysis plates and uses.

A method of detecting a membrane protein by mass spectrometry comprises: (a) providing a solution comprising a native membrane vesicle having a lipid bilayer to which said membrane protein is attached; (b) sonicating the vesicle in the presence of a mass spectrometry-compatible buffer; (c) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (d) vaporising the sonicated solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the vesicle; (e) ionising the membrane protein; (f) resolving the ionised membrane protein using the mass analyser; and (g) detecting the resolved membrane protein using the detector. Also provided are solutions comprising sonicated vesicles for use in mass spectrometry methods.

The present disclosure relates to compounds useful for the detection or modulation of target nucleic acids, including DNA and RNA. The present disclosure further relates to methods for treatment of trinucleotide repeat disorders, which can include administration of oligonucleotide analogues that can bind pathogenic nucleotide repeats in DNA or RNA.

A composition having the following structure:

##STR00001## wherein: R.sup.1 is OH, ester group, ether group, amine, thiol, thioether, halide, or a group containing an alkynyl or azido functionality; R.sup.2 is H, OH, ester group, ether group, amine, thiol, thioether, halide, or a group containing an alkynyl or azido functionality; R.sup.3 is a group containing a reactive functionality capable of covalent binding to a thiol or amine; and R.sup.4 is H, OH, ester group, ether group, amine, thiol, thioether, halide, or a group containing an alkynyl or azido functionality; wherein one of R.sup.1, R.sup.2 and R.sup.4 is a group containing an alkynyl or azido functionality. Also disclosed is a method for profiling changes in BSH enzyme activity by attaching active BSH enzymes in a sample to the probe shown above, attaching a tag to the probe, and detecting the active BSH enzymes to obtain an activity profile.

The present disclosure relates to a sensitive, multidimensional chromatography method for extraction, detection, and quantification of non-conjugated cytotoxic agents and associated linker molecules used in cysteine based antibody-drug-conjugate production.

A microorganism identification method utilizing mass spectrometry is provided. More specifically, a method for identifying a phylotype of Cutibacterium acnes utilizing mass spectrometry is provided. The method includes a) a step for reading out a m/z value of a peak derived from a marker protein on a mass spectrum which is obtained by mass spectrometry of a sample containing microorganisms; and b) a step for judging whether the sample contains Cutibacterium acnes (C. acnes) based on the m/z value.

Methods are described for using a combination of mass spectroscopic and proteomic approaches for identifying the specific pairing of heavy and light chains for an intact antibody, an antibody fragment, a mixture of intact antibodies, or a mixture of antibody fragments.

The present invention relates to a method of directly detecting, using a mass-spectrometry method, whether a microorganism contained in a sample is resistant to antibiotics, and a kit for detection used therewith. More particularly, the present invention relates to a method and kit for directly detecting an antibiotic hydrolase secreted by a microorganism resistant to antibiotics, thereby directly determining whether the microorganism is resistant to antibiotics. According to the present invention, it is possible to very simply and immediately confirm whether a specific strain is resistant to antibiotics in the field. In particular, a complicated pretreatment process such as proteolysis is not performed, and a complicated identification process of calibrating and then combining the obtained results is not performed. Accordingly, it is possible to realize a method of easily confirming whether antibiotic resistance occurs in just a dozen minutes, compared to a conventional technology in which it takes several days to confirm whether antibiotic resistance occurs, and a simple diagnostic kit used therewith.