This invention relates to the measurement of hemoglobin in the blood. Previously, chemical interference from medications, other blood components, and incomplete reagent rehydration can impact the accuracy and prolong the reaction time required to determine total hemoglobin in blood. Embodiments of the present invention use a disposable body is housed within the capillary channel and adapted to receive a flow of blood from the entrance such that air in the disposable body is pushed out through a vent. A measurement system is configured to measure a property of the flow of blood. That property can be correlated to total hemoglobin count.

A method for detecting an analyte reactive towards luminol, comprising the steps of: feeding into a reaction chamber an alkaline solution of luminol, noble metal nanoparticles and at least one analyte reactive towards luminol, wherein the reaction chamber is in the form of a curved channel; detecting the light emitted due to a chemiluminescence reaction taking place in said channel; and discharging a reaction mass from said channel, characterized in that the average diameter of the metal nanoparticles is greater than 25 nm. Also provided is a microfluidic device for carrying out the method.

Provided is a reagent that is suitable for measurement of a glycoprotein and contains a compound capable of stabilizing a leuco dye.

A method for determining the amount of glycated hemoglobin (HbA1c), in whichif requiredthe erythrocytes in a sample are hemolyzed, the haemoglobin that is then releasedif requiredis contacted with a proteolytic agent and the glycated hemoglobin degradation products obtained in this way or otherwise are quantified is disclosed. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, the provision of the requisite proteolytic agent in the form of an inactivated protease is proposed, which is then only reactivated in situ. For the stabilization of the hemoglobin, which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer should be present in the hemolysis solution, and, where a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed that the latter be stabilized with particular phosphine compounds and/or thio compounds.

A biosensor includes a base substrate; an electrode layer disposed on a main surface of the base substrate and including first and second electrode pairs, each pair including a working electrode and a counter electrode; a spacer substrate on the electrode layer and having first and openings, the first opening exposing a part of the first electrode pair and the second opening exposing a part of the second electrode pair; a first reagent layer containing a first reagent reactive with a sample and disposed in the first opening; a second reagent layer containing a second reagent reactive with the sample and disposed in the second opening; and a top cover substrate on the spacer substrate, the top cover substrate including an introduction portion having at least one introduction opening, the introduction portion overlapping a part of the first opening in the spacer substrate and a part of the second opening.

A chromogenic absorbent material for hemoglobin and glucose detection in an animal excretion is provided. The chromogenic absorbent material comprises a benzidine-type compound, an organic hydroperoxide, an oxidoreductase, a peroxidase, a pseudoperoxidase or a combination thereof, and a polysaccharide matrix, comprising: from about 10 wt % to about 32.5 wt % chemically or mechanically processed non-functionalized cellulose; from about 25 wt % to about 70 wt % pregelatinized starch (PGS); from 0 wt % to about 20 wt % guar gum; from 0 wt % to about 25 wt % methyl hydroxyethyl cellulose (MHEC); from 0 wt % to about 15 wt % hydroxyethyl cellulose (HEC); and from 0 wt % to about 10 wt % carboxymethyl cellulose (CMC).

The present disclosure relates to compositions and methods for diagnosis, research, and screening for chemicals in biological fluids (e.g., related to methanol poisoning). In particular, the present disclosure relates to point of care systems and methods for detecting formic acid or formate, in biological fluids by means of natural or recombinant formate oxidase.

A method for determining the amount of glycated haemoglobin (HbA1c), in whichif requiredthe erythrocytes in a sample are haemolysed, the haemoglobin that is then releasedif requiredis contacted with a proteolytic agent and the glycated haemoglobin degradation products obtained in this way or otherwise are quantified is disclosed. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, for the stabilization of the haemoglobin which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer is present in the haemolysis solution. Where a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed that the latter be stabilized with particular phosphine compounds and/or thio compounds, and, in particular embodiments, the requisite proteolytic agent is to be provided in the form of an inactivated protease which is then only reactivated in situ.

The present invention provides a method for measuring glycated hemoglobin in a sample, which comprises directly oxidizing glycated hemoglobin in a sample, and then measuring a substance produced or consumed by the oxidation; and a method for measuring glycated hemoglobin in a sample, which comprises directly oxidizing glycated hemoglobin in a sample using an enzyme, and then measuring a substance produced or consumed by the oxidation. A method for measuring glycated hemoglobin of the present invention is useful for diagnosing lifestyle-related disease such as diabetes mellitus.

The present invention provides a protein comprising an amino acid sequence in which arginine at position 61 of a protein comprising the amino acid sequence represented by SEQ ID NO: 1 is substituted to an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, proline, cysteine, methionine, asparagine, glutamine, and aspartic acid; and a method for measuring a glycated hemoglobin in a sample, wherein the method comprises reacting a glycated hemoglobin in a sample with a protease to produce a glycated hexapeptide, then reacting the produced glycated hexapeptide with the aforementioned protein, and measuring a substance produced or consumed by the reaction.