A sample dispatcher is disclosed and is configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a liquid separation system. The liquid separation system is configured for separating compounds of the sample fluids and comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase. The sample dispatcher comprises one or more sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof, and a bypass channel.

An apparatus for separating a fluidic sample includes a fluid drive arrangement including fluid drive units for driving a mobile phase along a flow path to a sample separation unit, a sample accommodation volume for accommodating the fluidic sample and selectively fluidically coupleable with or decoupleable from the flow path, and a control unit. The control unit is configured to control pressure decoupling of at least part of at least one of the fluid drive units from the flow path, and enable the partially pressure-decoupled fluid drive unit(s) to pressurize the sample accommodation volume before fluidically coupling the sample accommodation volume with the flow path and/or to de-pressurize the sample accommodation volume after fluidically coupling the sample accommodation volume with the flow path for preparing a subsequent intake of fluidic sample in the sample accommodation volume.

A sampling unit for handling a sample fluid includes a sample container having a length and being configured for receiving and storing the sample fluid, and a sample segment dispatching unit configured for providing a plurality of individual sample packages of the fluidic sample, each contained in a respective volume segment along the length of the sample container, and for individually dispatching each of the plurality of individual sample packages for further processing in a fluid processing unit.

A two-dimensional liquid chromatograph system includes a first dimension analysis part, a second dimension analysis part, a second dimension feeding device, a fraction introduction part, a shift gradient program creation part, a liquid feeding control part, and a shift timing adjustment part. The shift gradient program creation part is configured to create a shift gradient program for causing the second dimension feeding device to execute shift gradient liquid feeding. The shift timing adjustment part is configured to adjust a shift timing to each stage in the shift gradient program created by the shift gradient program creation part based on a preliminary chromatogram acquired by the first dimension detector before the series of analysis operations for the sample to be analyzed.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

A thermal modulator for gas chromatography includes an analytical capillary to be traversed by analytes and that is interposed between two gas chromatographic columns; a cooling system having a cold zone, a support element associated with the cold zone and supporting a portion of the analytical capillary at a corresponding or slightly higher temperature than the cold zone, so as to define a trapping portion of the analytes; a control system, which selectively controls the emission of pulsed current to electrically conductive elements associated with the analytical capillary to heat the trapping portion and cause the release or desorption of previously immobilized analytes; and a heating system of a portion of the analytical capillary, positioned outside of the support element and upstream of the support element, so as to generate a rapid expansion of the gas contained in the portion and facilitate the advancement of the released or desorbed analytes.

A chromatography system includes a first chromatography column for receiving and separating a flow stream, a plurality of traps configured to trap a plurality of distinct flow segments exiting the first chromatography column during separation of the flow stream, and a second chromatography column operatively associated with the plurality of traps for receiving and separating the distinct flow segments. The system can include at-column dilution at trapping and separating stages thereof. A chromatography method for operating the chromatographic system includes measuring a plurality of time segments corresponding to a plurality of peaks of a fluid sample flowing through the first chromatographic column, and sequentially fluidly coupling the plurality of distinct flow segments with the corresponding plurality of traps during time segments corresponding to the plurality of peaks.

Provided are two-dimensional chromatography systems and methods for separating and/or analyzing complex mixtures of organic compounds. In particularly, a two-dimensional reversed-phase liquid chromatography (RPLC)supercritical fluid chromatography (SFC) system is described including a trapping column at the interface which collects the analytes eluted from the first dimension chromatography while letting the RPLC mobile phase pass through. The peaks of interest from the RPLC dimension column are effectively focused as sharp concentration pulses on the trapping column, which is subsequently injected onto the second dimension SFC column. The system can be used for simultaneous achiral and chiral analysis of pharmaceutical compounds. The first dimension RPLC separation provides the achiral purity result, and the second dimension SFC separation provides the chiral purity result (enantiomeric excess).

A liquid chromatography having an on-line cleaning function, comprising a first flow channel (L3), a second flow channel (L21), an analysis flow channel (L22) and waste liquid flow channels, further comprising a cleaning flow channel (L25), a direction switch valve (V1) and a multi-flow channel switch valve (V2), etc. The liquid chromatography changes the flow path of the liquid by changing the communication relationship between the two-position switch valves, thus realizes the on-line cleaning function for a first chromatographic column (C1), a middle chromatographic column (C2), a filter or a protector (B2) respectively, and realize the simultaneous on-line cleaning function for the first chromatographic column (C1) and the middle chromatographic column (C2).

The present disclosure relates to a detection method for congeners of short-chain chlorinated paraffins as well as a device for realizing the detection method. The detection method includes the following steps: adding an internal standard substance to a test sample; subjecting the test sample to a separation process using a comprehensive two-dimensional gas chromatograph formed by connecting a non-polar or weak-polar column and a medium-polar column in series via a modulator; and detecting the sample by a mass analyzer employing a negative chemical ion source after the separation process. The method according to the present disclosure enables accurate qualitative analysis as well as accurate quantitative measurement for short-chain chlorinated paraffins. The detection is extremely accurate yet can be easily carried out with simple operations.