A reagent kit comprising a first polypeptide including a part in any one of amino acid sequences (A) to (C), and a second polypeptide including a part in any one of amino acid sequences (A) to (C), which are consistent of different sequences from a sequence of the first polypeptide; (A) an amino acid sequence in SEQ ID NO: 1 with deletion of an amino acid sequence from position 1 to 69 and an amino acid sequence from position 204 to 221, (B) an amino acid sequence in SEQ ID NO: 1 with deletion of an amino acid sequence from position 1 to 69 and deletion or substitution of at least one of amino acid residues at positions 146 to 156, (C) the amino acid sequence (A) or (B) with further deletion of at least one of amino acid residues at positions 70 to 74.

A reagent kit comprising a first polypeptide including a part in any one of amino acid sequences (A) to (C), and a second polypeptide including a part in any one of amino acid sequences (A) to (C), which are consistent of different sequences from a sequence of the first polypeptide; (A) an amino acid sequence in SEQ ID NO: 1 with deletion of an amino acid sequence from position 1 to 69 and an amino acid sequence from position 204 to 221, (B) an amino acid sequence in SEQ ID NO: 1 with deletion of an amino acid sequence from position 1 to 69 and deletion or substitution of at least one of amino acid residues at positions 146 to 156, (C) the amino acid sequence (A) or (B) with further deletion of at least one of amino acid residues at positions 70 to 74.

Nanozymes capped with a radical-scavenging capping agent are disclosed for use in biosensing assays with improved sensitivity. The radical-scavenging capping agent facilitates the capture and retention of one or more radicals for enhancing a catalytic reaction. In some example embodiments, the nanozyme capped by the radical-scavenging capping agent is capable of catalyzing the decomposition of hydrogen peroxide or molecular oxygen. The capped nanozymes may be incorporated with an electrode, such as the working electrode of an electrochemical sensor, for achieving enhanced catalytic activity and a lower limit of detection. In some example embodiments, the radical-scavenging capping agent is or includes thiocyanate. A rapid ethanol detection device and associated method are described in which the working electrode of an electrochemical sensor is modified by a peroxidase-mimetic nanozyme capped with a radical-scavenging capping agent for the enhanced generation of a reduction current associated with the decomposition of hydrogen peroxide.

Nanozymes capped with a radical-scavenging capping agent are disclosed for use in biosensing assays with improved sensitivity. The radical-scavenging capping agent facilitates the capture and retention of one or more radicals for enhancing a catalytic reaction. In some example embodiments, the nanozyme capped by the radical-scavenging capping agent is capable of catalyzing the decomposition of hydrogen peroxide or molecular oxygen. The capped nanozymes may be incorporated with an electrode, such as the working electrode of an electrochemical sensor, for achieving enhanced catalytic activity and a lower limit of detection. In some example embodiments, the radical-scavenging capping agent is or includes thiocyanate. A rapid ethanol detection device and associated method are described in which the working electrode of an electrochemical sensor is modified by a peroxidase-mimetic nanozyme capped with a radical-scavenging capping agent for the enhanced generation of a reduction current associated with the decomposition of hydrogen peroxide.

Two different antibodies that specifically bind to tau protein or phosphorylated tau protein are used. One of the two different antibodies is a first antibody immobilized on a support or labeled with a molecule capable of binding to the support, and the other of the two different antibodies is a second antibody labeled without being immobilized on the support. The tau protein includes an N-terminal domain, a C-terminal domain, and an intermediate domain located between the N-terminal and C-terminal domains. Epitopes recognized by the first and second antibodies are each an amino acid sequence contained in the intermediate domain or an amino acid sequence contained in the N-terminal domain. One of the first and second antibodies is first subjected to an antigen-antibody reaction with the blood sample, and the other of the first and second antibodies is subsequently subjected to an antigen-antibody reaction with the blood sample.

The present disclosure is directed to spherical nucleic acids (SNAs) comprising a nanoparticle core and an oligonucleotide, use of the SNAs to, e.g., detect target analytes, and methods of making the SNAs. In various embodiments, the target analyte is detected using the nanoparticle core, the oligonucleotide, or both. In some embodiments, the oligonucleotide comprises a detectable marker situated at an internal location within the oligonucleotide. In some aspects, the disclosure provides methods for detecting a target analyte comprising the step of contacting the target analyte with a spherical nucleic acid (SNA) and an agent, the SNA comprising a protein core and an oligonucleotide attached thereto, wherein the contacting of the protein core with the target analyte results in a change in the target analyte that is detectable by the agent, thereby detecting the target analyte.

The present disclosure is directed to spherical nucleic acids (SNAs) comprising a nanoparticle core and an oligonucleotide, use of the SNAs to, e.g., detect target analytes, and methods of making the SNAs. In various embodiments, the target analyte is detected using the nanoparticle core, the oligonucleotide, or both. In some embodiments, the oligonucleotide comprises a detectable marker situated at an internal location within the oligonucleotide. In some aspects, the disclosure provides methods for detecting a target analyte comprising the step of contacting the target analyte with a spherical nucleic acid (SNA) and an agent, the SNA comprising a protein core and an oligonucleotide attached thereto, wherein the contacting of the protein core with the target analyte results in a change in the target analyte that is detectable by the agent, thereby detecting the target analyte.

The present disclosure relates to a digital microfluidic chemiluminescence detection chip, a detection method and a detection device. The digital microfluidic chemiluminescence detection chip includes a first baseplate and a second baseplate disposed oppositely. A cavity formed by the first and second baseplate includes a mixing and incubating area for combining an antigen, a magnetic particle antibody and an antibody, a luminescence detection area for chemiluminescence and detecting an optical signal, and a communication path for communicating the mixing and incubating area and the luminescence detection area. The first baseplate is provided with a drive array for driving sample solution to move and an optical sensing array for acquiring a luminescence signal of the sample solution. The drive array corresponds to positions of the mixing and incubating area, the luminescence detection area and the communication path. The optical sensing array corresponds to a position of the luminescence detection area.

According to an embodiment, there are provided a screening culture medium that enables a convenient and quick examination for the presence of Candida auris, and a screening method. According to at least one embodiment, the screening culture medium contains an enzyme substrate, in which the screening culture medium is used for screening Candida auris based on an ability to degrade the enzyme substrate, the enzyme substrate including raffinose and xylose. According to another embodiment, the screening method uses the screening culture medium.

The application is to antibodies which have been labelled with polyenzymes (multiple enzymes), specifically polyperoxidases, for use in direct immunohistochemical assays of tissues. The antibodies used diagnostically may also be antibodies which are used therapeutically.