The invention discloses a method for in vitro testing of specimens, such as biomaterials, to obtain history-dependent, time-invariant functional materials properties using time-convolution and idempotent analysis. The purpose of the method is to measure these properties using a data processing without limitations of materials models, the properties linearity or material homogeneity.

The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.

The present disclosure is directed to antibody-linked immuno-sedimentation agent, the antibody being linked to a sedimentation agent by a non-antigen binding region of the antibody, and a method of isolating a target from a sample using the antibody-linked immuno-sedimentation agent. The methods involve forming a mixture including a sample with an antibody linked immuno-sedimentation agent and red blood cells under conditions sufficient to form red blood cell rouleaux and allow antibody-antigen binding.

An object of the present invention is to provide a method of reducing interference to a measurement system from water-soluble silicone and/or surfactant contaminating the measurement system in a latex agglutination immunoassay. The execution of a latex immunoagglutination reaction in the presence of a silicone compound can reduce the interference to the measurement system from a component derived from a micro blood-collection tube (water-soluble silicone) and/or surfactant mixed from outside of the measurement system.

A detection kit includes a photothermal conversion region that absorbs light and converts the light into heat. A plurality of pores are disposed in the photothermal conversion region. A detection method includes first to third steps. The first step is introducing a plurality of antibody-modified beads into the plurality of pores, each of the plurality of antibody-modified beads having a surface modified by an antibody capable of being specifically bound to an analyte. The second step is heating a sample to generate convection in the sample by irradiating the photothermal conversion region with light having a wavelength within an absorption wavelength range of the photothermal conversion region. The third step is detecting the analyte by monitoring the detection kit after the irradiation with the light.

An apparatus and method for separating a target material. The apparatus for separating a target matter includes a mixture including a target matter, a density gradient material layer disposed under the mixture and having a greater density than a density of the mixture, magnetic beads including a magnetic material and binding to the target matter to form a complex, and a magnetic field generating device applying a magnetic field to the complex to precipitate the complex at the bottom of the density gradient material layer.

The invention discloses a method for in vitro testing of specimens, such as biomaterials, to obtain history-dependent, time-invariant functional materials properties using time-convolution and idempotent analysis. The purpose of the method is to measure these properties using a data processing without limitations of materials models, the properties linearity or material homogeneity.

Herein is reported a method for determining the binding affinity of the binding sites of a bivalent full length antibody of the human IgG1 subclass to a homo-multimeric antigen comprising the steps of i) incubating a mixture comprising the antibody and a polypeptide that is derived from lysine-gingipain of porphyromonas gingivalis at a pH of from pH 7.5 to pH 8.5, in the presence of a reducing agent, at a temperature of from 30 C. to 42 C., for time of from 10 min. to 240 min. to cleave the antibody into Fabs and Fc-region, and ii) determining the binding affinity of the Fabs of the antibody for its antigen using a surface plasmon resonance method by directly applying the incubated reaction mixture obtained in the previous step in the surface plasmon resonance method and therewith determining the binding affinity of the binding sites of the bivalent full length antibody of the human IgG1 subclass.

A method for detecting a presence and/or a concentration of a target substance in a reagent solution using enzyme-linked immunosorbent assay (ELISA) includes binding the target substance directly or indirectly to an electrode, and binding a detection agent directly or indirectly to the bound target substance. The method further includes modulating a pH of only a portion of the reagent solution in which the bound target substance and the bound detection agent are located using the electrode, the modulated pH of the portion of the reagent solution causing the bound detection agent to undergo a change, and detecting the change in the bound detection agent. The detected change corresponds to the presence of the target substance in the reagent solution and/or the concentration of the target substance in the reagent solution.

A method for detecting a presence and/or a concentration of a target substance in a reagent solution using enzyme-linked immunosorbent assay (ELISA) includes binding the target substance directly or indirectly to an electrode, and binding a detection agent directly or indirectly to the bound target substance. The method further includes modulating a pH of only a portion of the reagent solution in which the bound target substance and the bound detection agent are located using the electrode, the modulated pH of the portion of the reagent solution causing the bound detection agent to undergo a change, and detecting the change in the bound detection agent. The detected change corresponds to the presence of the target substance in the reagent solution and/or the concentration of the target substance in the reagent solution.