Acoustically responsive particles and methods are provided for their use. Methods are provided for making and using tunable, monodisperse acoustically responsive particles and negative contrast acoustic particles, wherein the particles can contain a functional group available for covalent modification.

Acoustically responsive particles and methods are provided for their use. Methods are provided for making and using tunable, monodisperse acoustically responsive particles and negative contrast acoustic particles, wherein the particles can contain a functional group available for covalent modification.

Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample.

Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample.

Disclosed is a SARS-CoV-2 IgG/IgM detection kit. The kit includes a disposable test cassette and the disposable test cassette includes a base plate, a sample pad, a reaction pad and an absorbent pad. The sample pad, the reaction pad and the absorbent pad are sequentially connected and are provided on the base plate. The sample pad is coated with a colloidal gold-labeled recombinant SARS-CoV-2 S-RBD protein. The reaction pad is provided with a test line and a quality control line. The test line is coated with a mouse anti-human IgG and a mouse anti-human IgM and the quality control line is coated with a goat anti-mouse IgG. The kit provided herein can be used for rapid test of SARS-CoV-2 IgG/IgM, and has high accuracy, good stability and strong anti-interference performance.

Disclosed is a SARS-CoV-2 IgG/IgM detection kit. The kit includes a disposable test cassette and the disposable test cassette includes a base plate, a sample pad, a reaction pad and an absorbent pad. The sample pad, the reaction pad and the absorbent pad are sequentially connected and are provided on the base plate. The sample pad is coated with a colloidal gold-labeled recombinant SARS-CoV-2 S-RBD protein. The reaction pad is provided with a test line and a quality control line. The test line is coated with a mouse anti-human IgG and a mouse anti-human IgM and the quality control line is coated with a goat anti-mouse IgG. The kit provided herein can be used for rapid test of SARS-CoV-2 IgG/IgM, and has high accuracy, good stability and strong anti-interference performance.

A host antigen-specific antibody testing system and method. The a ternary complex of the antigen, a ligand-bound anti-host IgM, and a non-host anti-antigen IgG detector conjugate selectively form a quaternary complex with host antibodies, wherein the host antibodies and IgG compete for the antigen, and the anti-host IgM binds the host antibodies. The quaternary complex is retained by an immobilized IgM ligand binding agent, and any residual ternary complex is retained by a later encountered immobilized anti-non-host IgG. If sufficient host antibodies have a high affinity for the antigen, the complex is detected at the quaternary complex detection region based on the presence of the detector, and if there are insufficient high affinity host antibodies, the ternary complex migrates past the quaternary complex detection region and is retained and detected at a control region.

The present disclosure relates to CO.sub.2-based chromatography for the efficient and precise separation of Vitamin D metabolites.

According to an aspect, a server receives first and second transient (changing with time) locations of an entity, the first transient location being associated with a time instance until which the entity is physically present at the first transient location. The server accordingly provides the first transient location as the physical location of the entity prior to the time instance, and the second transient location as the physical location after the time instance. According to another aspect, a client device identifies that a search text (received from a user) is directed to searching for physical locations in view of the search text including an affix. The client device then parses the search text to determine an identifier, sends a location request containing the identifier, receives a response containing a transient location associated with the identifier, and then provides the transient location to the user as the response to the search.

According to an aspect, a server receives first and second transient (changing with time) locations of an entity, the first transient location being associated with a time instance until which the entity is physically present at the first transient location. The server accordingly provides the first transient location as the physical location of the entity prior to the time instance, and the second transient location as the physical location after the time instance. According to another aspect, a client device identifies that a search text (received from a user) is directed to searching for physical locations in view of the search text including an affix. The client device then parses the search text to determine an identifier, sends a location request containing the identifier, receives a response containing a transient location associated with the identifier, and then provides the transient location to the user as the response to the search.