The present disclosure relates to a method for producing a TLR5 agonist protein. According to the present disclosure, the TLR5 agonist protein can be easily separated and purified after biotechnological production. In particular, the fusion partner used for separation and purification is effectively removed so as to minimize the possibility of inhibiting binding to TLR5 and inducing an immune response by the fusion partner.

The present disclosure relates to a method for producing a TLR5 agonist protein. According to the present disclosure, the TLR5 agonist protein can be easily separated and purified after biotechnological production. In particular, the fusion partner used for separation and purification is effectively removed so as to minimize the possibility of inhibiting binding to TLR5 and inducing an immune response by the fusion partner.

The present disclosure relates to a method and a kit for analyzing a nucleic acid amplification product, and more particularly to a method and kit of analyzing a nucleic acid amplification product using a labeled primer comprising (i) a binding region complementary to the target nucleic acid, (ii) a nucleic acid oligomer containing nucleotides non-complementary to the target nucleic acid, and (iii) a label, and using a membrane having immobilized thereon a probe that binds complementarily to the nucleic acid oligomer.

The present disclosure relates to a method and a kit for analyzing a nucleic acid amplification product, and more particularly to a method and kit of analyzing a nucleic acid amplification product using a labeled primer comprising (i) a binding region complementary to the target nucleic acid, (ii) a nucleic acid oligomer containing nucleotides non-complementary to the target nucleic acid, and (iii) a label, and using a membrane having immobilized thereon a probe that binds complementarily to the nucleic acid oligomer.

Determining the relative binding capacity of albumin (A), and amount of functional albumin, involves at least two measurement solutions of a test and reference sample. The measurement solutions contain an albumin-binding marker M. The marker in the measurement solution of the test and reference samples exceeds the presumed available albumin binding capacity. The test sample contains a defined amount of albumin of unknown binding capacity. The reference sample contains the same defined amount of albumin having a reference binding capacity. The measurement solutions are incubated under conditions that allow M:A complexes to form. The M:A complexes are removed. The presence or amount of unbound marker M in the solutions is detected after M:A complex removal by a test strip that allows determination of the unbound marker. The relative binding capacity of albumin in the test sample based on the presence or detected amounts of unbound marker is determined.

A fluid-tightly sealable sampling device for analysis of one or more substances in a fluid flow intended to pass through the sampling device is disclosed, wherein it comprises an adsorption device (1) which is hollow and is adapted to be provided with one or more reagents for adsorption of and reaction with said one or more substances in the fluid flow, a filter holder (2), which is hollow and is connected in a fluid-tight way with the adsorption device (1), a filter device (4) adapted to be provided with one or more reagents for adsorption of and reaction with said one or more substances in the fluid flow, a gasket (5) provided with at least one projection (6) engaged with at least one corresponding receiving slot in the filter holder (2), a first external cap (9) detachably connected with the inlet end of the sampling device in a fluid-tight way and a second external cap (13) detachably connected with the outlet end of the sampling device in a fluid-tight way.

A fluid-tightly sealable sampling device for analysis of one or more substances in a fluid flow intended to pass through the sampling device is disclosed, wherein it comprises an adsorption device (1) which is hollow and is adapted to be provided with one or more reagents for adsorption of and reaction with said one or more substances in the fluid flow, a filter holder (2), which is hollow and is connected in a fluid-tight way with the adsorption device (1), a filter device (4) adapted to be provided with one or more reagents for adsorption of and reaction with said one or more substances in the fluid flow, a gasket (5) provided with at least one projection (6) engaged with at least one corresponding receiving slot in the filter holder (2), a first external cap (9) detachably connected with the inlet end of the sampling device in a fluid-tight way and a second external cap (13) detachably connected with the outlet end of the sampling device in a fluid-tight way.

Provided herein are compositions and methods for sorting and/or identifying live cells. The compositions and methods provide for staining of live cells with aptamer so particular cells can be identified within or sorted from a heterogeneous population of live cells and subsequent reversal of the staining to prepare sorted and/or identified cells in their native state.

In some embodiments, the present invention is a device, including: a swab including an absorptive component attached to a stem, an extraction chamber configured to receive the swab and position the absorptive component of the swab configured to be in fluid communication with an extraction reagent, a test strip configured to be brought in fluid communication with the extraction reagent following extraction of the analyte from the biological sample, including: a sample receiving portion configured to accept a sample, a site on the strip where the analyte-specific labeled reagent has been incorporated, such reagent configured to bind the analyte from the biological sample, a capture portion configured to receive: the analyte from the biological sample and the analyte-specific labeled reagent so as to result in displaying a positive or negative result at the completion of the assay, and an adsorbent pad attached to the test strip.

In some embodiments, the present invention is a device, including: a swab including an absorptive component attached to a stem, an extraction chamber configured to receive the swab and position the absorptive component of the swab configured to be in fluid communication with an extraction reagent, a test strip configured to be brought in fluid communication with the extraction reagent following extraction of the analyte from the biological sample, including: a sample receiving portion configured to accept a sample, a site on the strip where the analyte-specific labeled reagent has been incorporated, such reagent configured to bind the analyte from the biological sample, a capture portion configured to receive: the analyte from the biological sample and the analyte-specific labeled reagent so as to result in displaying a positive or negative result at the completion of the assay, and an adsorbent pad attached to the test strip.