This disclosure provides kits and methods for detecting markers in a sample from a subject with unknown status and generating a risk assessment of the presence or absence of cancer, such as colorectal cancer. In embodiments, a kit comprises at least two reagents, each specifically binding to one of at least two polypeptides in a sample from the subject. The polypeptides include IL-8 and ferritin. The kit further includes at least one standard comprising a known amount of at least one of the polypeptides. The kit can also include computer readable media comprising instructions to analyze the detected amounts of the at least two polypeptides using a machine learning algorithm to determine whether a subject has an increased risk of the presence of colorectal cancer.

A diagnostic system for determining the presence of a target in a sample liquid that includes a diagnostic reader and a microfluidic strip having a microfluidic channel network therein. An actuator within the reader modifies the pressure of a gas in gaseous communication with a liquid-gas interface of a sample liquid within the microfluidic channel network to move and/or mix the sample liquid. The pressure modifications may be continuous and/or oscillatory.

A magnetic particle (30, 70) has a layered structure (6, 56) between a top surface of the particle and an opposed bottom surface of the particle. Layers of the structure include one or more nonmagnetic layer(s) and one or more magnetized layer(s). The ratio of a lateral dimension of the one or more magnetized layers to the aggregate thickness of the magnetized layer or layers is greater than 500. A plurality of such magnetic particles (30, 70) can be functionalised and marked with readable codes (16, 66) corresponding to the functionalisation, for use for performing assays such as bioassays.

The present invention provides systems, devices, and methods for point-of-care and/or distributed testing services. The methods and devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device can be modified to allow for more flexible and robust use with the disclosed methods for a variety of medical, laboratory, and other applications. The systems, devices, and methods of the present invention can allow for effective use of samples by improved sample preparation and analysis.

A method of sample preparation for streamlined multi-step assays is provided. The method includes the step of providing a microfluidic device including a reservoir defined by a surface configured to repel an aqueous solution. A dried reagent is provided on a portion of the surface and the reservoir is filled with an oil. A first droplet formed from the aqueous solution is positioned on the dried reagent so to pick-up and re-dissolve the dried reagent therein so as to expose the portion of the surface. In addition, a second droplet of an aqueous solution may be deposited on a hydrophilic spot patterned on the surface. A magnetic force may be configured to interact magnetically with the paramagnetic beads within the first droplet to move the droplet through the oil in the reservoir or to move the paramagnetic beads from the first droplet, through the oil, into the second droplet.

The present invention relates to a method for separating biomolecules from a liquid medium. The method comprises adding magnetic nanoparticles to the liquid medium comprising the biomolecules, the biomolecules each adapted to bind to respective surfaces of the magnetic nanoparticles; bringing the liquid medium to which the magnetic nanoparticles have been added into contact with a collector; applying a magnetic field to the liquid medium in contact with the collector to attract the magnetic nanoparticles ound with the biomolecules to a surface of the collector; and applying an electric potential to the surface of the collector to release the biomolecules from the magnetic nanoparticles.

Systems and methods for automated sample preparation and processing of protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic agents.

The invention provides methods for enriching extracellular vesicles (EVs), including exosomes, from biological fluid samples from subjects, and optionally further testing the EVs for the presence of specific biomarkers.

A bioassay system includes at least one conductive excitation coil, the at least one conductive excitation coil configured to generate an alternating magnetic field including a first frequency and a second frequency. The bioassay system further includes a sample mount configured to position a sample within the at least one conductive excitation coil, and at least one sensing conductive coil configured to determine a magnetic response of a sample positioned within the sample mount to the alternating magnetic field.

A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution. A method of capturing a sample substance that is bound to a carrier particle, using a fluidic device which includes a circulation flow path and a capture part arranged on the circulation flow path and configured to capture the carrier particle and in which the circulation flow path has two or more circulation flow path valves, includes: an introduction step of, in a state where the circulation flow path valve is closed, introducing a solution that includes a sample substance to at least one of partitions partitioned by the circulation flow path valve and introducing a solution that includes a carrier particle which is bound to the sample substance to at least another of the partitions; a mix step of opening all of the circulation flow path valves and circulating and mixing a solution in the circulation flow path; and a capture step of capturing the carrier particle by the capture part.