Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.

An integrated microfluidic chromatin immunoprecipitation assay dramatically improves the collection efficiency of ChIP DNA from cells. Immunoprecipitation of chromatin fragments is conducted in a microfluidic chamber with a large fraction of its volume (e.g., ˜15-40%) occupied by magnetic immunoprecipitation (IP) beads. Oscillating washing of the beads, enabled by, e.g., solenoid valves (controlled by a computer) and high pressure attached to both ends of the microfluidic chamber, effectively removes unbound chromatin and produces high-quality ChIP DNA. ChIP DNA produced by an example device generates excellent results in the subsequent DNA library preparation. The ChIP-seq (i.e., ChIP followed by next-generation sequencing) results match very well with public data generated using much larger cell sample sizes and a conventional approach.

Provided is a magnetic particle excellent in detection speed when a substance to be measured, such as an antigen or an antibody, is detected from a specimen. The particle includes a magnetic particle containing a magnetic material, wherein the magnetic particle has a resin on a surface, wherein the particle has a volume average particle diameter of 0.4 μm or more and 1.5 μm or less, wherein the particle has a density of 5.1 g/cm.sup.3 or more and 10.0 g/cm.sup.3 or less, and wherein the resin has a functional group capable of binding a ligand.

SUSD2 protein is used as a marker in identification, selection or separation of pancreatic internal secretion precursor cells and/or newborn pancreatic internal secretion cells; and a use of an mRNA, for encoding the SUSD2 protein, of a precursor protein as the marker in identification of the pancreatic internal secretion precursor cells and/or the newborn pancreatic internal secretion cells. Analysis of gene expression of pancreatic endoderm cells sourced by induced directional differentiation of human pluripotent stem cells finds the enrichment expression of a SUSD2 gene in the pancreatic internal secretion precursor cells and the newborn pancreatic internal secretion cells. A protein encoded by the SUSD2 gene is a receptor protein on cell membranes. Using the protein as the marker, the identification, the selection or the separation of the pancreatic internal secretion precursor cells and the newborn pancreatic internal secretion cells can be conducted.

A uniform cluster of nanocompositions suspended in a liquid media is provided. Methods of making such nanocompositions, and uses of such nanocompositions are also provided. The nanocompositions can be used for nucleic acid extraction and diagnostic assays, for immunoassays, for cell separation, identification and modulation, for controlled functional molecule protection and release, for assays used in the clinic (companion diagnostics) or in the therapeutic development process (drug target validation), and in a system for transcatheter arterial chemoembolization, and demonstrate superior performance due to the uniform property or monodispersity.

Disclosed is a method for measuring a viral antigen using a capture antibody and a detection antibody, the method comprising forming a sandwich immune complex that contains the capture antibody, the viral antigen and the detection antibody, the capture antibody comprising a heavy chain variable region that comprises CDR1 comprising amino acid sequence of SEQ ID NO: 1, CDR2 comprising amino acid sequence of SEQ ID NO: 2 and CDR3 comprising amino acid sequence of SEQ ID NO: 3, and a light chain variable region that comprises CDR1 comprising amino acid sequence of SEQ ID NO: 4, CDR2 comprising amino acid sequence of SEQ ID NO: 5 and CDR3 comprising amino acid sequence of SEQ ID NO: 6, and the detection antibody comprising a heavy chain variable region that comprises CDR1 comprising amino acid sequence of SEQ ID NO: 7, CDR2 comprising amino acid sequence of SEQ ID NO: 8 and CDR3 comprising amino acid sequence of SEQ ID NO: 9, and a light chain variable region that comprises CDR1 comprising amino acid sequence of SEQ ID NO: 10, CDR2 comprising amino acid sequence of SEQ ID NO: 11 and CDR3 comprising amino acid sequence of SEQ ID NO: 12.

The present invention provides an urinary crystal detection method, which is used with a crystal collecting unit and a crystal detecting unit. The method comprises steps of using magnetic particles to attach urinary crystals from a sample. Then, providing a magnetic field to separate the urinary crystals. Further to analyz their constituent by the Raman signals of the urinary crystal to access an urinary calculus result for urinary stone patient.

Early stage, rapid, low-cost detection of disease components in a biological sample is critically important. A point of care device can include a collection region and can be used to hold a sample that is combined with a fluorescent dye and a plurality of magnetic particles such that disease components in the sample are tagged with the fluorescent dye and the plurality of magnetic particles. At least one magnet can be located next to the collection region to establish a magnetic field gradient to draw the tagged disease components into the collection region from the device. A fluorescence microscope can image the small collection region based on the fluorescent dye to detect the disease components. The fluorescence microscope uses light to excite the fluorescent dye and a filter to transmit light emitted by the fluorescent dye to the fluorescence microscope, while restricting light used to excite the fluorescent dye.

An analysis tool for use mounted to an analysis device that automatically analyzes a specified component contained in a sample. The analysis tool includes a light measurement well to hold a measurement solution as a measurement subject, and to measure the measurement solution. The light measurement well includes an opening to dispense the measurement solution through, a measurement solution holder to hold the measurement solution dispensed through the opening, and an emission section that emits measurement light caused to be emitted from the measurement solution held in the measurement solution holder in a light receiving direction of the analysis device. The measurement light is fluorescent light or chemiluminescent light. The measurement solution holder has a flattened profile that is flattened in the light receiving direction.

The present invention relates to composite particles, coated particles, a method of producing composite particles, a ligand-containing solid phase carrier, and a method of detecting or separating a target substance in a sample. The above described composite particles each contains an organic polymer and inorganic nanoparticles, wherein the content of the inorganic nanoparticles in the composite particles is more than 80% by mass, and wherein the composite particles have a volume average particle size of from 10 to 1,000 nm.